Discovery of dopamine glucuronide in rat and mouse brain microdialysis samples using liquid chromatography tandem mass spectrometry

Päivi Susanna Uutela, Laura Karhu, Timo Petteri Piepponen, Mikko Käenmäki, Raimo Ketola, Risto Kostiainen

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    A liquid chromatographic-electrospray/tandem mass spectrometric (LC-ESI-MS/MS) method was developed for the analysis of dopamine and its phase I and phase II metabolites from brain microdialysis samples. The method provides for the first time the analysis of intact dopamine glucuronide and sulfate without hydrolysis. The paper describes also an enzymatic synthesis method using rat liver microsomes as biocatalysts and characterization of dopamine glucuronide as a reference compound. The method was validated for quantitative analysis by determining limits of detection and quantitation, linearity, repeatability, and specificity. Dopamine glucuronide was found for the first time in rat and mouse brain microdialysis samples. The concentrations of dopamine and its glucuronide in the microdialysates collected from the striatum of rat brains were approximately equal (2 nM). Dopamine sulfate was not detected in the microdialysates (limit of detection 0.8 nM). The main metabolites of dopamine were dihydroxyphenylacetic acid (DOPAC, 1200 nM) and homovanillic acid (HVA, 700 nM).
    Original languageEnglish
    JournalAnalytical Chemistry
    Volume2009
    Issue number81
    Pages (from-to)427-434
    ISSN0003-2700
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • 317 Pharmacy

    Cite this

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    title = "Discovery of dopamine glucuronide in rat and mouse brain microdialysis samples using liquid chromatography tandem mass spectrometry",
    abstract = "A liquid chromatographic-electrospray/tandem mass spectrometric (LC-ESI-MS/MS) method was developed for the analysis of dopamine and its phase I and phase II metabolites from brain microdialysis samples. The method provides for the first time the analysis of intact dopamine glucuronide and sulfate without hydrolysis. The paper describes also an enzymatic synthesis method using rat liver microsomes as biocatalysts and characterization of dopamine glucuronide as a reference compound. The method was validated for quantitative analysis by determining limits of detection and quantitation, linearity, repeatability, and specificity. Dopamine glucuronide was found for the first time in rat and mouse brain microdialysis samples. The concentrations of dopamine and its glucuronide in the microdialysates collected from the striatum of rat brains were approximately equal (2 nM). Dopamine sulfate was not detected in the microdialysates (limit of detection 0.8 nM). The main metabolites of dopamine were dihydroxyphenylacetic acid (DOPAC, 1200 nM) and homovanillic acid (HVA, 700 nM).",
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    author = "Uutela, {P{\"a}ivi Susanna} and Laura Karhu and Piepponen, {Timo Petteri} and Mikko K{\"a}enm{\"a}ki and Raimo Ketola and Risto Kostiainen",
    year = "2009",
    doi = "10.1021/ac801846w",
    language = "English",
    volume = "2009",
    pages = "427--434",
    journal = "Analytical Chemistry",
    issn = "0003-2700",
    publisher = "American Chemical Society Journals",
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    Discovery of dopamine glucuronide in rat and mouse brain microdialysis samples using liquid chromatography tandem mass spectrometry. / Uutela, Päivi Susanna; Karhu, Laura; Piepponen, Timo Petteri; Käenmäki, Mikko; Ketola, Raimo; Kostiainen, Risto.

    In: Analytical Chemistry, Vol. 2009, No. 81, 2009, p. 427-434.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Discovery of dopamine glucuronide in rat and mouse brain microdialysis samples using liquid chromatography tandem mass spectrometry

    AU - Uutela, Päivi Susanna

    AU - Karhu, Laura

    AU - Piepponen, Timo Petteri

    AU - Käenmäki, Mikko

    AU - Ketola, Raimo

    AU - Kostiainen, Risto

    PY - 2009

    Y1 - 2009

    N2 - A liquid chromatographic-electrospray/tandem mass spectrometric (LC-ESI-MS/MS) method was developed for the analysis of dopamine and its phase I and phase II metabolites from brain microdialysis samples. The method provides for the first time the analysis of intact dopamine glucuronide and sulfate without hydrolysis. The paper describes also an enzymatic synthesis method using rat liver microsomes as biocatalysts and characterization of dopamine glucuronide as a reference compound. The method was validated for quantitative analysis by determining limits of detection and quantitation, linearity, repeatability, and specificity. Dopamine glucuronide was found for the first time in rat and mouse brain microdialysis samples. The concentrations of dopamine and its glucuronide in the microdialysates collected from the striatum of rat brains were approximately equal (2 nM). Dopamine sulfate was not detected in the microdialysates (limit of detection 0.8 nM). The main metabolites of dopamine were dihydroxyphenylacetic acid (DOPAC, 1200 nM) and homovanillic acid (HVA, 700 nM).

    AB - A liquid chromatographic-electrospray/tandem mass spectrometric (LC-ESI-MS/MS) method was developed for the analysis of dopamine and its phase I and phase II metabolites from brain microdialysis samples. The method provides for the first time the analysis of intact dopamine glucuronide and sulfate without hydrolysis. The paper describes also an enzymatic synthesis method using rat liver microsomes as biocatalysts and characterization of dopamine glucuronide as a reference compound. The method was validated for quantitative analysis by determining limits of detection and quantitation, linearity, repeatability, and specificity. Dopamine glucuronide was found for the first time in rat and mouse brain microdialysis samples. The concentrations of dopamine and its glucuronide in the microdialysates collected from the striatum of rat brains were approximately equal (2 nM). Dopamine sulfate was not detected in the microdialysates (limit of detection 0.8 nM). The main metabolites of dopamine were dihydroxyphenylacetic acid (DOPAC, 1200 nM) and homovanillic acid (HVA, 700 nM).

    KW - 317 Pharmacy

    U2 - 10.1021/ac801846w

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    M3 - Article

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    EP - 434

    JO - Analytical Chemistry

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    ER -