Discovery of neurosteroid glucuronides in mouse brain

Sirkku Kallonen, Anne Tammimäki, Petteri Piepponen, Helena Raattamaa, Raimo A Ketola, Risto Kostiainen

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    Neurosteroid glucuronides were found for the first time in brain samples. The intact glucuronides were extracted from the cortex, hippocampus, hypothalamus, and mid-brain tissues of nicotine- and water-treated mice, and detected with capillary liquid chromatography-electrospray-tandem mass spectrometry (CapLC-ESI-MS/MS). The glucuronides of estradiol, cortisol, corticosterone, tetrahydrodeoxycorticosterone, pregnenolone, and isopregnanolone were identified by comparing retention times in selected reaction monitoring (SRM) chromatograms and the relative abundances of two SRM transitions of each neurosteroid glucuronide between the reference and authentic samples, thus providing reliable identification. In vitro experiments, carried out by using S9 fractions from mouse and rat brains, showed a formation of glucuronides with selected test compounds (corticosterone, pregnenolone, and dehydroepiandrosterone), suggesting that biosynthesis of neurosteroid glucuronides is possible in rodent brain. (C) 2009 Elsevier B.V. All rights reserved.
    Original languageEnglish
    JournalAnalytica Chimica Acta
    Volume651
    Issue number1
    Pages (from-to)69-74
    Number of pages6
    ISSN0003-2670
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Cite this

    Kallonen, Sirkku ; Tammimäki, Anne ; Piepponen, Petteri ; Raattamaa, Helena ; Ketola, Raimo A ; Kostiainen, Risto. / Discovery of neurosteroid glucuronides in mouse brain. In: Analytica Chimica Acta. 2009 ; Vol. 651, No. 1. pp. 69-74.
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    title = "Discovery of neurosteroid glucuronides in mouse brain",
    abstract = "Neurosteroid glucuronides were found for the first time in brain samples. The intact glucuronides were extracted from the cortex, hippocampus, hypothalamus, and mid-brain tissues of nicotine- and water-treated mice, and detected with capillary liquid chromatography-electrospray-tandem mass spectrometry (CapLC-ESI-MS/MS). The glucuronides of estradiol, cortisol, corticosterone, tetrahydrodeoxycorticosterone, pregnenolone, and isopregnanolone were identified by comparing retention times in selected reaction monitoring (SRM) chromatograms and the relative abundances of two SRM transitions of each neurosteroid glucuronide between the reference and authentic samples, thus providing reliable identification. In vitro experiments, carried out by using S9 fractions from mouse and rat brains, showed a formation of glucuronides with selected test compounds (corticosterone, pregnenolone, and dehydroepiandrosterone), suggesting that biosynthesis of neurosteroid glucuronides is possible in rodent brain. (C) 2009 Elsevier B.V. All rights reserved.",
    author = "Sirkku Kallonen and Anne Tammim{\"a}ki and Petteri Piepponen and Helena Raattamaa and Ketola, {Raimo A} and Risto Kostiainen",
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    Discovery of neurosteroid glucuronides in mouse brain. / Kallonen, Sirkku; Tammimäki, Anne; Piepponen, Petteri; Raattamaa, Helena; Ketola, Raimo A; Kostiainen, Risto.

    In: Analytica Chimica Acta, Vol. 651, No. 1, 2009, p. 69-74.

    Research output: Contribution to journalArticleScientificpeer-review

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    AU - Kallonen, Sirkku

    AU - Tammimäki, Anne

    AU - Piepponen, Petteri

    AU - Raattamaa, Helena

    AU - Ketola, Raimo A

    AU - Kostiainen, Risto

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    AB - Neurosteroid glucuronides were found for the first time in brain samples. The intact glucuronides were extracted from the cortex, hippocampus, hypothalamus, and mid-brain tissues of nicotine- and water-treated mice, and detected with capillary liquid chromatography-electrospray-tandem mass spectrometry (CapLC-ESI-MS/MS). The glucuronides of estradiol, cortisol, corticosterone, tetrahydrodeoxycorticosterone, pregnenolone, and isopregnanolone were identified by comparing retention times in selected reaction monitoring (SRM) chromatograms and the relative abundances of two SRM transitions of each neurosteroid glucuronide between the reference and authentic samples, thus providing reliable identification. In vitro experiments, carried out by using S9 fractions from mouse and rat brains, showed a formation of glucuronides with selected test compounds (corticosterone, pregnenolone, and dehydroepiandrosterone), suggesting that biosynthesis of neurosteroid glucuronides is possible in rodent brain. (C) 2009 Elsevier B.V. All rights reserved.

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