Divergence between the highly virulent zoonotic pathogen Helicobacter heilmannii and its closest relative, the low-virulence “Helicobacter ailurogastricus” sp. nov.

Myrthe Joosten, Sara Lindén, Mirko Rossi, Alfred Chin Yen Tay, Emma Skoog, Médea Padra, Fanny Peters, Tim Perkins, Peter Vandamme, Filip Van Nieuwerburgh, Katharina D'Herde, Wim Van den Broeck, Bram Flahou, Dieter Deforce, Richard Ducatelle, Barry Marshall, Freddy Haesebrouck, Annemieke Smet

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Helicobacter heilmannii naturally colonizes the stomach of dogs and cats, and has been associated with gastric disorders in humans. Nine feline Helicobacter strains, classified as H. heilmannii based on ureAB and 16S rRNA gene sequences, were divided into a highly virulent and a low virulent group. The genomes of these strains were sequenced to investigate their phylogenetic relationships, to define their gene content and diversity and to determine if the differences in pathogenicity were associated with the presence/absence of potential virulence genes. The binding capacity of these helicobacters to the gastric mucosa was investigated as well.Our analyses revealed that the low virulent strains do not belong to the H. heilmannii species, but to a novel, closely related species for which we propose the name H. ailurogastricus. Several homologs of H. pylori virulence factors, such as IceA1, HrgA and jhp0562-like glycosyltransferase, are present in H. heilmannii but absent in H. ailurogastricus. Both species contain a VacA-like autotransporter, from which the passenger domain is remarkably larger for H. ailurogastricus compared to H. heilmannii. In addition, H. ailurogastricus shows clear differences in binding to the gastric mucosa compared to H. heilmannii. These findings highlight the low virulent character of this novel Helicobacter species.
Original languageEnglish
JournalInfection and Immunity
Volume84
Issue number1
Pages (from-to)293-306
Number of pages14
ISSN0019-9567
DOIs
Publication statusPublished - Jan 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 413 Veterinary science
  • OUTER-MEMBRANE PROTEINS
  • MULTIPLE SEQUENCE ALIGNMENT
  • GASTRIC EPITHELIAL-CELLS
  • COMPARATIVE GENOMICS
  • SUBSYSTEMS TECHNOLOGY
  • PHASE VARIATION
  • PYLORI BINDING
  • MALT LYMPHOMA
  • BIZZOZERONII
  • INFECTION

Cite this

Joosten, Myrthe ; Lindén, Sara ; Rossi, Mirko ; Chin Yen Tay, Alfred ; Skoog, Emma ; Padra, Médea ; Peters, Fanny ; Perkins, Tim ; Vandamme, Peter ; Van Nieuwerburgh, Filip ; D'Herde, Katharina ; Van den Broeck, Wim ; Flahou, Bram ; Deforce, Dieter ; Ducatelle, Richard ; Marshall, Barry ; Haesebrouck, Freddy ; Smet, Annemieke. / Divergence between the highly virulent zoonotic pathogen Helicobacter heilmannii and its closest relative, the low-virulence “Helicobacter ailurogastricus” sp. nov. In: Infection and Immunity. 2016 ; Vol. 84, No. 1. pp. 293-306.
@article{e12a1e23b76348cd9f7b3f5383a101fe,
title = "Divergence between the highly virulent zoonotic pathogen Helicobacter heilmannii and its closest relative, the low-virulence “Helicobacter ailurogastricus” sp. nov.",
abstract = "Helicobacter heilmannii naturally colonizes the stomach of dogs and cats, and has been associated with gastric disorders in humans. Nine feline Helicobacter strains, classified as H. heilmannii based on ureAB and 16S rRNA gene sequences, were divided into a highly virulent and a low virulent group. The genomes of these strains were sequenced to investigate their phylogenetic relationships, to define their gene content and diversity and to determine if the differences in pathogenicity were associated with the presence/absence of potential virulence genes. The binding capacity of these helicobacters to the gastric mucosa was investigated as well.Our analyses revealed that the low virulent strains do not belong to the H. heilmannii species, but to a novel, closely related species for which we propose the name H. ailurogastricus. Several homologs of H. pylori virulence factors, such as IceA1, HrgA and jhp0562-like glycosyltransferase, are present in H. heilmannii but absent in H. ailurogastricus. Both species contain a VacA-like autotransporter, from which the passenger domain is remarkably larger for H. ailurogastricus compared to H. heilmannii. In addition, H. ailurogastricus shows clear differences in binding to the gastric mucosa compared to H. heilmannii. These findings highlight the low virulent character of this novel Helicobacter species.",
keywords = "413 Veterinary science, OUTER-MEMBRANE PROTEINS, MULTIPLE SEQUENCE ALIGNMENT, GASTRIC EPITHELIAL-CELLS, COMPARATIVE GENOMICS, SUBSYSTEMS TECHNOLOGY, PHASE VARIATION, PYLORI BINDING, MALT LYMPHOMA, BIZZOZERONII, INFECTION",
author = "Myrthe Joosten and Sara Lind{\'e}n and Mirko Rossi and {Chin Yen Tay}, Alfred and Emma Skoog and M{\'e}dea Padra and Fanny Peters and Tim Perkins and Peter Vandamme and {Van Nieuwerburgh}, Filip and Katharina D'Herde and {Van den Broeck}, Wim and Bram Flahou and Dieter Deforce and Richard Ducatelle and Barry Marshall and Freddy Haesebrouck and Annemieke Smet",
year = "2016",
month = "1",
doi = "10.1128/IAI.01300-15",
language = "English",
volume = "84",
pages = "293--306",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "1",

}

Joosten, M, Lindén, S, Rossi, M, Chin Yen Tay, A, Skoog, E, Padra, M, Peters, F, Perkins, T, Vandamme, P, Van Nieuwerburgh, F, D'Herde, K, Van den Broeck, W, Flahou, B, Deforce, D, Ducatelle, R, Marshall, B, Haesebrouck, F & Smet, A 2016, 'Divergence between the highly virulent zoonotic pathogen Helicobacter heilmannii and its closest relative, the low-virulence “Helicobacter ailurogastricus” sp. nov.' Infection and Immunity, vol. 84, no. 1, pp. 293-306. https://doi.org/10.1128/IAI.01300-15

Divergence between the highly virulent zoonotic pathogen Helicobacter heilmannii and its closest relative, the low-virulence “Helicobacter ailurogastricus” sp. nov. / Joosten, Myrthe; Lindén, Sara; Rossi, Mirko; Chin Yen Tay, Alfred; Skoog, Emma; Padra, Médea; Peters, Fanny; Perkins, Tim; Vandamme, Peter; Van Nieuwerburgh, Filip; D'Herde, Katharina; Van den Broeck, Wim; Flahou, Bram; Deforce, Dieter; Ducatelle, Richard; Marshall, Barry; Haesebrouck, Freddy; Smet, Annemieke.

In: Infection and Immunity, Vol. 84, No. 1, 01.2016, p. 293-306.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Divergence between the highly virulent zoonotic pathogen Helicobacter heilmannii and its closest relative, the low-virulence “Helicobacter ailurogastricus” sp. nov.

AU - Joosten, Myrthe

AU - Lindén, Sara

AU - Rossi, Mirko

AU - Chin Yen Tay, Alfred

AU - Skoog, Emma

AU - Padra, Médea

AU - Peters, Fanny

AU - Perkins, Tim

AU - Vandamme, Peter

AU - Van Nieuwerburgh, Filip

AU - D'Herde, Katharina

AU - Van den Broeck, Wim

AU - Flahou, Bram

AU - Deforce, Dieter

AU - Ducatelle, Richard

AU - Marshall, Barry

AU - Haesebrouck, Freddy

AU - Smet, Annemieke

PY - 2016/1

Y1 - 2016/1

N2 - Helicobacter heilmannii naturally colonizes the stomach of dogs and cats, and has been associated with gastric disorders in humans. Nine feline Helicobacter strains, classified as H. heilmannii based on ureAB and 16S rRNA gene sequences, were divided into a highly virulent and a low virulent group. The genomes of these strains were sequenced to investigate their phylogenetic relationships, to define their gene content and diversity and to determine if the differences in pathogenicity were associated with the presence/absence of potential virulence genes. The binding capacity of these helicobacters to the gastric mucosa was investigated as well.Our analyses revealed that the low virulent strains do not belong to the H. heilmannii species, but to a novel, closely related species for which we propose the name H. ailurogastricus. Several homologs of H. pylori virulence factors, such as IceA1, HrgA and jhp0562-like glycosyltransferase, are present in H. heilmannii but absent in H. ailurogastricus. Both species contain a VacA-like autotransporter, from which the passenger domain is remarkably larger for H. ailurogastricus compared to H. heilmannii. In addition, H. ailurogastricus shows clear differences in binding to the gastric mucosa compared to H. heilmannii. These findings highlight the low virulent character of this novel Helicobacter species.

AB - Helicobacter heilmannii naturally colonizes the stomach of dogs and cats, and has been associated with gastric disorders in humans. Nine feline Helicobacter strains, classified as H. heilmannii based on ureAB and 16S rRNA gene sequences, were divided into a highly virulent and a low virulent group. The genomes of these strains were sequenced to investigate their phylogenetic relationships, to define their gene content and diversity and to determine if the differences in pathogenicity were associated with the presence/absence of potential virulence genes. The binding capacity of these helicobacters to the gastric mucosa was investigated as well.Our analyses revealed that the low virulent strains do not belong to the H. heilmannii species, but to a novel, closely related species for which we propose the name H. ailurogastricus. Several homologs of H. pylori virulence factors, such as IceA1, HrgA and jhp0562-like glycosyltransferase, are present in H. heilmannii but absent in H. ailurogastricus. Both species contain a VacA-like autotransporter, from which the passenger domain is remarkably larger for H. ailurogastricus compared to H. heilmannii. In addition, H. ailurogastricus shows clear differences in binding to the gastric mucosa compared to H. heilmannii. These findings highlight the low virulent character of this novel Helicobacter species.

KW - 413 Veterinary science

KW - OUTER-MEMBRANE PROTEINS

KW - MULTIPLE SEQUENCE ALIGNMENT

KW - GASTRIC EPITHELIAL-CELLS

KW - COMPARATIVE GENOMICS

KW - SUBSYSTEMS TECHNOLOGY

KW - PHASE VARIATION

KW - PYLORI BINDING

KW - MALT LYMPHOMA

KW - BIZZOZERONII

KW - INFECTION

U2 - 10.1128/IAI.01300-15

DO - 10.1128/IAI.01300-15

M3 - Article

VL - 84

SP - 293

EP - 306

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 1

ER -