DNA-origami-directed virus capsid polymorphism

Iris Seitz; Sharon Saarinen; Esa Pekka Kumpula; Donna McNeale; Eduardo Anaya-Plaza; Vili Lampinen; Vesa P. Hytönen; Frank Sainsbury; Jeroen J. L. M. Cornelissen; Veikko Linko; Juha T. Huiskonen; Mauri A. Kostiainen

doi:10.1038/s41565-023-01443-x

DNA-origami-directed virus capsid polymorphism

Iris Seitz, Sharon Saarinen, Esa Pekka Kumpula, Donna McNeale, Eduardo Anaya-Plaza, Vili Lampinen, Vesa P. Hytönen, Frank Sainsbury, Jeroen J. L. M. Cornelissen, Veikko Linko, Juha T. Huiskonen, Mauri A. Kostiainen

Research output: Contribution to journal › Article › Scientific › peer-review

Original language	English
Journal	Nature Nanotechnology
Volume	18
Issue number	10
Pages (from-to)	1205–1212
ISSN	1748-3387
DOIs	https://doi.org/10.1038/s41565-023-01443-x
Publication status	Published - Oct 2023
MoE publication type	A1 Journal article-refereed

Bibliographical note

Funding Information:
The authors acknowledge financial support from the European Research Council (ERC) and ERA Chair MATTER under the European Union’s Horizon 2020 research and innovation programme (grant agreement numbers 101002258 (M.A.K.) and 856705 (V. Linko)), the Emil Aaltonen Foundation (V. Linko), the Sigrid Jusélius Foundation (V. Linko), the Academy of Finland (grant numbers 341057 (E.A.-P.) and 314671 (M.A.K)), the Finnish Foundation for Technology Promotion (V. Lampinen), and the Jane and Aatos Erkko Foundation (V. Linko and M.A.K.). This work was carried out under the Academy of Finland Centers of Excellence Program (2022-2029) in Life-Inspired Hybrid Materials (LIBER), project number number 346110 (M.A.K.). K. M. Nguyen and A. Kuzyk are acknowledged for providing the staple strands for the 13HR sample, P. Laurinmäki and B. Löflund for technical assistance with cryo-EM, M. Hankaniemi for support with NoV production, and M. Sammalkorpi and A. Scacchi for technical discussions. The facilities and expertise of the HiLIFE cryo-EM unit at the University of Helsinki, a member of Instruct-ERIC Centre Finland, FINStruct and Biocenter Finland are gratefully acknowledged. The authors also acknowledge CSC–IT Center of Science, Finland, for computational resources, Biocenter Finland for support of protein production and characterization infrastructure, and the provision of facilities and technical support by Aalto University Bioeconomy Facilities, OtaNanoNanomicroscopy Center (Aalto-NMC) and Micronova Nanofabrication Center.

Publisher Copyright:
© 2023, The Author(s).

Fields of Science

Assemblies
Core
Cowpea chlorotic mottle
Encapsulation
Folding dna
Forms
Nanostructures
Protein
Simian-virus-40
Spherical virus
1182 Biochemistry, cell and molecular biology

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s41565-023-01443-xFinal published version, 4.69 MBLicence: CC BY

Cite this

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title = "DNA-origami-directed virus capsid polymorphism",

keywords = "Assemblies, Core, Cowpea chlorotic mottle, Encapsulation, Folding dna, Forms, Nanostructures, Protein, Simian-virus-40, Spherical virus, 1182 Biochemistry, cell and molecular biology",

author = "Iris Seitz and Sharon Saarinen and Kumpula, {Esa Pekka} and Donna McNeale and Eduardo Anaya-Plaza and Vili Lampinen and Hyt{\"o}nen, {Vesa P.} and Frank Sainsbury and Cornelissen, {Jeroen J. L. M.} and Veikko Linko and Huiskonen, {Juha T.} and Kostiainen, {Mauri A.}",

note = "Funding Information: The authors acknowledge financial support from the European Research Council (ERC) and ERA Chair MATTER under the European Union{\textquoteright}s Horizon 2020 research and innovation programme (grant agreement numbers 101002258 (M.A.K.) and 856705 (V. Linko)), the Emil Aaltonen Foundation (V. Linko), the Sigrid Jus{\'e}lius Foundation (V. Linko), the Academy of Finland (grant numbers 341057 (E.A.-P.) and 314671 (M.A.K)), the Finnish Foundation for Technology Promotion (V. Lampinen), and the Jane and Aatos Erkko Foundation (V. Linko and M.A.K.). This work was carried out under the Academy of Finland Centers of Excellence Program (2022-2029) in Life-Inspired Hybrid Materials (LIBER), project number number 346110 (M.A.K.). K. M. Nguyen and A. Kuzyk are acknowledged for providing the staple strands for the 13HR sample, P. Laurinm{\"a}ki and B. L{\"o}flund for technical assistance with cryo-EM, M. Hankaniemi for support with NoV production, and M. Sammalkorpi and A. Scacchi for technical discussions. The facilities and expertise of the HiLIFE cryo-EM unit at the University of Helsinki, a member of Instruct-ERIC Centre Finland, FINStruct and Biocenter Finland are gratefully acknowledged. The authors also acknowledge CSC–IT Center of Science, Finland, for computational resources, Biocenter Finland for support of protein production and characterization infrastructure, and the provision of facilities and technical support by Aalto University Bioeconomy Facilities, OtaNanoNanomicroscopy Center (Aalto-NMC) and Micronova Nanofabrication Center. Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

month = oct,

doi = "10.1038/s41565-023-01443-x",

language = "English",

volume = "18",

pages = "1205–1212",

journal = "Nature Nanotechnology",

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TY - JOUR

T1 - DNA-origami-directed virus capsid polymorphism

AU - Seitz, Iris

AU - Saarinen, Sharon

AU - Kumpula, Esa Pekka

AU - McNeale, Donna

AU - Anaya-Plaza, Eduardo

AU - Lampinen, Vili

AU - Hytönen, Vesa P.

AU - Sainsbury, Frank

AU - Cornelissen, Jeroen J. L. M.

AU - Linko, Veikko

AU - Huiskonen, Juha T.

AU - Kostiainen, Mauri A.

N1 - Funding Information: The authors acknowledge financial support from the European Research Council (ERC) and ERA Chair MATTER under the European Union’s Horizon 2020 research and innovation programme (grant agreement numbers 101002258 (M.A.K.) and 856705 (V. Linko)), the Emil Aaltonen Foundation (V. Linko), the Sigrid Jusélius Foundation (V. Linko), the Academy of Finland (grant numbers 341057 (E.A.-P.) and 314671 (M.A.K)), the Finnish Foundation for Technology Promotion (V. Lampinen), and the Jane and Aatos Erkko Foundation (V. Linko and M.A.K.). This work was carried out under the Academy of Finland Centers of Excellence Program (2022-2029) in Life-Inspired Hybrid Materials (LIBER), project number number 346110 (M.A.K.). K. M. Nguyen and A. Kuzyk are acknowledged for providing the staple strands for the 13HR sample, P. Laurinmäki and B. Löflund for technical assistance with cryo-EM, M. Hankaniemi for support with NoV production, and M. Sammalkorpi and A. Scacchi for technical discussions. The facilities and expertise of the HiLIFE cryo-EM unit at the University of Helsinki, a member of Instruct-ERIC Centre Finland, FINStruct and Biocenter Finland are gratefully acknowledged. The authors also acknowledge CSC–IT Center of Science, Finland, for computational resources, Biocenter Finland for support of protein production and characterization infrastructure, and the provision of facilities and technical support by Aalto University Bioeconomy Facilities, OtaNanoNanomicroscopy Center (Aalto-NMC) and Micronova Nanofabrication Center. Publisher Copyright: © 2023, The Author(s).

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KW - Assemblies

KW - Core

KW - Cowpea chlorotic mottle

KW - Encapsulation

KW - Folding dna

KW - Forms

KW - Nanostructures

KW - Protein

KW - Simian-virus-40

KW - Spherical virus

KW - 1182 Biochemistry, cell and molecular biology

U2 - 10.1038/s41565-023-01443-x

DO - 10.1038/s41565-023-01443-x

M3 - Article

C2 - 37460794

AN - SCOPUS:85164964063

SN - 1748-3387

VL - 18

SP - 1205

EP - 1212

JO - Nature Nanotechnology

JF - Nature Nanotechnology

IS - 10

ER -