Do faba bean (Vicia faba L.) accessions from environments with contrasting seasonal moisture availabilities differ in stomatal characteristics and related traits?

Hamid Khazaei, Kenneth Street, Arja Santanen, Abdallah Bari, Fred Stoddard

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Drought is a major constraint to faba bean (Vicia faba L.) production, and there are many mechanisms by which leaves can regulate water loss. Our primary objective was to test if the origin of the faba bean accessions, from drought-prone and non-drought-prone environments, was associated with differences in measurable aspects of stomatal morphology and physiology related to water use. Two sets, each consisting of 201 faba bean accessions, were chosen from environments with contrasting seasonal moisture profiles following the Focused Identification of Germplasm Strategy (FIGS), and then screened under well watered conditions. From these, two subsets of 10 accessions each were chosen to test for differences in response to drought. Parameters related to stomatal function and water status were measured. The dry-adapted set had bigger stomata, higher leaf relative water content (LRWC) and cooler leaves under well watered conditions. Stomatal density and stomatal area per unit area of leaflet were negatively correlated with gas exchange parameters and positively correlated with intrinsic water use efficiency. Drought caused stomatal densities to increase in the dry set while stomatal length decreased in both sets. The moisture deficit was sufficient to decrease gas exchange in both sets to similar levels, but the dry-adapted set maintained warmer leaves and a higher LRWC that showed no significant correlations with leaf morphology or gas exchange, demonstrating more effective stomatal regulation. The results also support that collection site data from the environment where genetic resources are collected can be used as indicators of adaptive traits in an herbaceous annual species.
Original languageEnglish
JournalGenetic Resources and Crop Evolution
Volume60
Issue number8
Pages (from-to)2343-2357
Number of pages15
ISSN0925-9864
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 4111 Agronomy
  • Crop breeding
  • Plant genetic resources
  • Crop physiology
  • Drought

Cite this

@article{df0a19d9e55e473195a44621242c3132,
title = "Do faba bean (Vicia faba L.) accessions from environments with contrasting seasonal moisture availabilities differ in stomatal characteristics and related traits?",
abstract = "Drought is a major constraint to faba bean (Vicia faba L.) production, and there are many mechanisms by which leaves can regulate water loss. Our primary objective was to test if the origin of the faba bean accessions, from drought-prone and non-drought-prone environments, was associated with differences in measurable aspects of stomatal morphology and physiology related to water use. Two sets, each consisting of 201 faba bean accessions, were chosen from environments with contrasting seasonal moisture profiles following the Focused Identification of Germplasm Strategy (FIGS), and then screened under well watered conditions. From these, two subsets of 10 accessions each were chosen to test for differences in response to drought. Parameters related to stomatal function and water status were measured. The dry-adapted set had bigger stomata, higher leaf relative water content (LRWC) and cooler leaves under well watered conditions. Stomatal density and stomatal area per unit area of leaflet were negatively correlated with gas exchange parameters and positively correlated with intrinsic water use efficiency. Drought caused stomatal densities to increase in the dry set while stomatal length decreased in both sets. The moisture deficit was sufficient to decrease gas exchange in both sets to similar levels, but the dry-adapted set maintained warmer leaves and a higher LRWC that showed no significant correlations with leaf morphology or gas exchange, demonstrating more effective stomatal regulation. The results also support that collection site data from the environment where genetic resources are collected can be used as indicators of adaptive traits in an herbaceous annual species.",
keywords = "4111 Agronomy, Crop breeding, Plant genetic resources, Crop physiology, Drought",
author = "Hamid Khazaei and Kenneth Street and Arja Santanen and Abdallah Bari and Fred Stoddard",
year = "2013",
doi = "10.1007/s10722-013-0002-4",
language = "English",
volume = "60",
pages = "2343--2357",
journal = "Genetic Resources and Crop Evolution",
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Do faba bean (Vicia faba L.) accessions from environments with contrasting seasonal moisture availabilities differ in stomatal characteristics and related traits? / Khazaei, Hamid; Street, Kenneth ; Santanen, Arja; Bari, Abdallah ; Stoddard, Fred.

In: Genetic Resources and Crop Evolution, Vol. 60, No. 8, 2013, p. 2343-2357.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Do faba bean (Vicia faba L.) accessions from environments with contrasting seasonal moisture availabilities differ in stomatal characteristics and related traits?

AU - Khazaei, Hamid

AU - Street, Kenneth

AU - Santanen, Arja

AU - Bari, Abdallah

AU - Stoddard, Fred

PY - 2013

Y1 - 2013

N2 - Drought is a major constraint to faba bean (Vicia faba L.) production, and there are many mechanisms by which leaves can regulate water loss. Our primary objective was to test if the origin of the faba bean accessions, from drought-prone and non-drought-prone environments, was associated with differences in measurable aspects of stomatal morphology and physiology related to water use. Two sets, each consisting of 201 faba bean accessions, were chosen from environments with contrasting seasonal moisture profiles following the Focused Identification of Germplasm Strategy (FIGS), and then screened under well watered conditions. From these, two subsets of 10 accessions each were chosen to test for differences in response to drought. Parameters related to stomatal function and water status were measured. The dry-adapted set had bigger stomata, higher leaf relative water content (LRWC) and cooler leaves under well watered conditions. Stomatal density and stomatal area per unit area of leaflet were negatively correlated with gas exchange parameters and positively correlated with intrinsic water use efficiency. Drought caused stomatal densities to increase in the dry set while stomatal length decreased in both sets. The moisture deficit was sufficient to decrease gas exchange in both sets to similar levels, but the dry-adapted set maintained warmer leaves and a higher LRWC that showed no significant correlations with leaf morphology or gas exchange, demonstrating more effective stomatal regulation. The results also support that collection site data from the environment where genetic resources are collected can be used as indicators of adaptive traits in an herbaceous annual species.

AB - Drought is a major constraint to faba bean (Vicia faba L.) production, and there are many mechanisms by which leaves can regulate water loss. Our primary objective was to test if the origin of the faba bean accessions, from drought-prone and non-drought-prone environments, was associated with differences in measurable aspects of stomatal morphology and physiology related to water use. Two sets, each consisting of 201 faba bean accessions, were chosen from environments with contrasting seasonal moisture profiles following the Focused Identification of Germplasm Strategy (FIGS), and then screened under well watered conditions. From these, two subsets of 10 accessions each were chosen to test for differences in response to drought. Parameters related to stomatal function and water status were measured. The dry-adapted set had bigger stomata, higher leaf relative water content (LRWC) and cooler leaves under well watered conditions. Stomatal density and stomatal area per unit area of leaflet were negatively correlated with gas exchange parameters and positively correlated with intrinsic water use efficiency. Drought caused stomatal densities to increase in the dry set while stomatal length decreased in both sets. The moisture deficit was sufficient to decrease gas exchange in both sets to similar levels, but the dry-adapted set maintained warmer leaves and a higher LRWC that showed no significant correlations with leaf morphology or gas exchange, demonstrating more effective stomatal regulation. The results also support that collection site data from the environment where genetic resources are collected can be used as indicators of adaptive traits in an herbaceous annual species.

KW - 4111 Agronomy

KW - Crop breeding

KW - Plant genetic resources

KW - Crop physiology

KW - Drought

U2 - 10.1007/s10722-013-0002-4

DO - 10.1007/s10722-013-0002-4

M3 - Article

VL - 60

SP - 2343

EP - 2357

JO - Genetic Resources and Crop Evolution

JF - Genetic Resources and Crop Evolution

SN - 0925-9864

IS - 8

ER -