Do UV-A radiation and blue light during growth prime leaves to cope with acute high-light in photoreceptor mutants of Arabidopsis thaliana?

Craig Brelsford, Luis Orlando Morales Suarez, Jakub Nezval, Titta Katariina Kotilainen, Saara Maria Hartikainen, Pedro J. Aphalo, Thomas Matthew Robson

Research output: Contribution to journalArticleScientificpeer-review

Abstract

We studied how plants acclimated to growing conditions that included combinations of blue light (BL) and ultraviolet (UV)-A radiation, and whether their growing environment affected their photosynthetic capacity during and after a brief period of acute high light (as might happen during an under-canopy sunfleck). Arabidopsis thaliana Landsberg erecta wild-type were compared with mutants lacking functional blue light and UV photoreceptors: phototropin 1, cryptochromes (CRY1 and CRY2) and UV RESISTANT LOCUS 8 (uvr8). This was achieved using light-emitting-diode (LED) lamps in a controlled environment to create treatments with or without BL, in a split-plot design with or without UV-A radiation. We compared the accumulation of phenolic compounds under growth conditions and after exposure to 30 min of high light at the end of the experiment (46 days), and likewise measured the operational efficiency of photosystem II (phi PSII, a proxy for photosynthetic performance) and dark-adapted maximum quantum yield (F-v/F-m to assess PSII damage). Our results indicate that cryptochromes are the main photoreceptors regulating phenolic compound accumulation in response to BL and UV-A radiation, and a lack of functional cryptochromes impairs photosynthetic performance under high light. Our findings also reveal a role for UVR8 in accumulating flavonoids in response to a low UV-A dose. Interestingly, phototropin 1 partially mediated constitutive accumulation of phenolic compounds in the absence of BL. Low-irradiance BL and UV-A did not improve phi PSII and F-v/F-m upon our acute high-light treatment; however, CRYs played an important role in ameliorating high-light stress.

Original languageEnglish
JournalPhysiologia Plantarum
Volume165
Issue number3
Pages (from-to)537-554
Number of pages18
ISSN0031-9317
DOIs
Publication statusPublished - Mar 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • SYNTHASE GENE-EXPRESSION
  • PHOTOSYNTHETICALLY ACTIVE RADIATION
  • ULTRAVIOLET-B RADIATION
  • SIGNAL-TRANSDUCTION
  • PHOTOSYSTEM-II
  • HIGHER-PLANTS
  • CHLOROPHYLL FLUORESCENCE
  • ELECTRON-TRANSPORT
  • LEAF CHLOROPHYLL
  • RED-LIGHT

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