DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW

Wouter Smet, Iris Sevilem, MA de Luis Balaguer, B Wybouw, E Mor, Shunsuke Miyashima, Bernhard Blob, Pawel Roszak, TB Jacobs, M Boekschoten, G Hooiveld, Rosangela Sozzani, Yrjö Eero Helariutta, Bert De Rybel

Research output: Contribution to journalArticleScientificpeer-review

Abstract

To create a three-dimensional structure, plants rely on oriented cell divisions and cell elongation. Oriented cell divisions are specifically important in procambium cells of the root to establish the different vascular cell types [1, 2]. These divisions are in part controlled by the auxin-controlled TARGET OF MONOPTEROS5 (TMO5) and LONESOME HIGHWAY (LHW) transcription factor complex [3-7]. Loss-of-function of tmo5 or lhw clade members results in strongly reduced vascular cell file numbers, whereas ectopic expression of both TMO5 and LHW can ubiquitously induce periclinal and radial cell divisions in all cell types of the root meristem. TMO5 and LHW interact only in young xylem cells, where they promote expression of two direct target genes involved in the final step of cytokinin (CK) biosynthesis, LONELY GUY3 (LOG3) and LOG4 [8, 9] Therefore, CK was hypothesized to act as a mobile signal from the xylem to trigger divisions in the neighboring procambium cells [3, 6]. To unravel how TMO5/LHW-dependent cytokinin regulates cell proliferation, we analyzed the transcriptional responses upon simultaneous induction of both transcription factors. Using inferred network analysis, we identified AT2G28510/DOF2.1 as a cytokinin-dependent downstream target gene. We further showed that DOF2.1 controls specific procambium cell divisions without inducing other cytokinin-dependent effects such as the inhibition of vascular differentiation. In summary, our results suggest that DOF2.1 and its closest homologs control vascular cell proliferation, thus leading to radial expansion of the root.
Original languageEnglish
JournalCurrent Biology
Volume29
Issue number3
Pages (from-to)520-+
Number of pages16
ISSN0960-9822
DOIs
Publication statusPublished - 4 Feb 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • ARABIDOPSIS
  • DIFFERENTIATION
  • DIVISION
  • EXPRESSION
  • GENE
  • INITIATION
  • LIGATION-INDEPENDENT CLONING
  • ORGANIZATION
  • PATHWAY
  • RESPONSE REGULATOR
  • 1182 Biochemistry, cell and molecular biology

Cite this

Smet, W., Sevilem, I., de Luis Balaguer, MA., Wybouw, B., Mor, E., Miyashima, S., ... De Rybel, B. (2019). DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW. Current Biology, 29(3), 520-+. https://doi.org/10.1016/j.cub.2018.12.041
Smet, Wouter ; Sevilem, Iris ; de Luis Balaguer, MA ; Wybouw, B ; Mor, E ; Miyashima, Shunsuke ; Blob, Bernhard ; Roszak, Pawel ; Jacobs, TB ; Boekschoten, M ; Hooiveld, G ; Sozzani, Rosangela ; Helariutta, Yrjö Eero ; De Rybel, Bert. / DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW. In: Current Biology. 2019 ; Vol. 29, No. 3. pp. 520-+.
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title = "DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW",
abstract = "To create a three-dimensional structure, plants rely on oriented cell divisions and cell elongation. Oriented cell divisions are specifically important in procambium cells of the root to establish the different vascular cell types [1, 2]. These divisions are in part controlled by the auxin-controlled TARGET OF MONOPTEROS5 (TMO5) and LONESOME HIGHWAY (LHW) transcription factor complex [3-7]. Loss-of-function of tmo5 or lhw clade members results in strongly reduced vascular cell file numbers, whereas ectopic expression of both TMO5 and LHW can ubiquitously induce periclinal and radial cell divisions in all cell types of the root meristem. TMO5 and LHW interact only in young xylem cells, where they promote expression of two direct target genes involved in the final step of cytokinin (CK) biosynthesis, LONELY GUY3 (LOG3) and LOG4 [8, 9] Therefore, CK was hypothesized to act as a mobile signal from the xylem to trigger divisions in the neighboring procambium cells [3, 6]. To unravel how TMO5/LHW-dependent cytokinin regulates cell proliferation, we analyzed the transcriptional responses upon simultaneous induction of both transcription factors. Using inferred network analysis, we identified AT2G28510/DOF2.1 as a cytokinin-dependent downstream target gene. We further showed that DOF2.1 controls specific procambium cell divisions without inducing other cytokinin-dependent effects such as the inhibition of vascular differentiation. In summary, our results suggest that DOF2.1 and its closest homologs control vascular cell proliferation, thus leading to radial expansion of the root.",
keywords = "ARABIDOPSIS, DIFFERENTIATION, DIVISION, EXPRESSION, GENE, INITIATION, LIGATION-INDEPENDENT CLONING, ORGANIZATION, PATHWAY, RESPONSE REGULATOR, 1182 Biochemistry, cell and molecular biology",
author = "Wouter Smet and Iris Sevilem and {de Luis Balaguer}, MA and B Wybouw and E Mor and Shunsuke Miyashima and Bernhard Blob and Pawel Roszak and TB Jacobs and M Boekschoten and G Hooiveld and Rosangela Sozzani and Helariutta, {Yrj{\"o} Eero} and {De Rybel}, Bert",
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Smet, W, Sevilem, I, de Luis Balaguer, MA, Wybouw, B, Mor, E, Miyashima, S, Blob, B, Roszak, P, Jacobs, TB, Boekschoten, M, Hooiveld, G, Sozzani, R, Helariutta, YE & De Rybel, B 2019, 'DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW', Current Biology, vol. 29, no. 3, pp. 520-+. https://doi.org/10.1016/j.cub.2018.12.041

DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW. / Smet, Wouter; Sevilem, Iris; de Luis Balaguer, MA; Wybouw, B; Mor, E; Miyashima, Shunsuke; Blob, Bernhard; Roszak, Pawel; Jacobs, TB; Boekschoten, M; Hooiveld, G; Sozzani, Rosangela; Helariutta, Yrjö Eero; De Rybel, Bert.

In: Current Biology, Vol. 29, No. 3, 04.02.2019, p. 520-+.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW

AU - Smet, Wouter

AU - Sevilem, Iris

AU - de Luis Balaguer, MA

AU - Wybouw, B

AU - Mor, E

AU - Miyashima, Shunsuke

AU - Blob, Bernhard

AU - Roszak, Pawel

AU - Jacobs, TB

AU - Boekschoten, M

AU - Hooiveld, G

AU - Sozzani, Rosangela

AU - Helariutta, Yrjö Eero

AU - De Rybel, Bert

PY - 2019/2/4

Y1 - 2019/2/4

N2 - To create a three-dimensional structure, plants rely on oriented cell divisions and cell elongation. Oriented cell divisions are specifically important in procambium cells of the root to establish the different vascular cell types [1, 2]. These divisions are in part controlled by the auxin-controlled TARGET OF MONOPTEROS5 (TMO5) and LONESOME HIGHWAY (LHW) transcription factor complex [3-7]. Loss-of-function of tmo5 or lhw clade members results in strongly reduced vascular cell file numbers, whereas ectopic expression of both TMO5 and LHW can ubiquitously induce periclinal and radial cell divisions in all cell types of the root meristem. TMO5 and LHW interact only in young xylem cells, where they promote expression of two direct target genes involved in the final step of cytokinin (CK) biosynthesis, LONELY GUY3 (LOG3) and LOG4 [8, 9] Therefore, CK was hypothesized to act as a mobile signal from the xylem to trigger divisions in the neighboring procambium cells [3, 6]. To unravel how TMO5/LHW-dependent cytokinin regulates cell proliferation, we analyzed the transcriptional responses upon simultaneous induction of both transcription factors. Using inferred network analysis, we identified AT2G28510/DOF2.1 as a cytokinin-dependent downstream target gene. We further showed that DOF2.1 controls specific procambium cell divisions without inducing other cytokinin-dependent effects such as the inhibition of vascular differentiation. In summary, our results suggest that DOF2.1 and its closest homologs control vascular cell proliferation, thus leading to radial expansion of the root.

AB - To create a three-dimensional structure, plants rely on oriented cell divisions and cell elongation. Oriented cell divisions are specifically important in procambium cells of the root to establish the different vascular cell types [1, 2]. These divisions are in part controlled by the auxin-controlled TARGET OF MONOPTEROS5 (TMO5) and LONESOME HIGHWAY (LHW) transcription factor complex [3-7]. Loss-of-function of tmo5 or lhw clade members results in strongly reduced vascular cell file numbers, whereas ectopic expression of both TMO5 and LHW can ubiquitously induce periclinal and radial cell divisions in all cell types of the root meristem. TMO5 and LHW interact only in young xylem cells, where they promote expression of two direct target genes involved in the final step of cytokinin (CK) biosynthesis, LONELY GUY3 (LOG3) and LOG4 [8, 9] Therefore, CK was hypothesized to act as a mobile signal from the xylem to trigger divisions in the neighboring procambium cells [3, 6]. To unravel how TMO5/LHW-dependent cytokinin regulates cell proliferation, we analyzed the transcriptional responses upon simultaneous induction of both transcription factors. Using inferred network analysis, we identified AT2G28510/DOF2.1 as a cytokinin-dependent downstream target gene. We further showed that DOF2.1 controls specific procambium cell divisions without inducing other cytokinin-dependent effects such as the inhibition of vascular differentiation. In summary, our results suggest that DOF2.1 and its closest homologs control vascular cell proliferation, thus leading to radial expansion of the root.

KW - ARABIDOPSIS

KW - DIFFERENTIATION

KW - DIVISION

KW - EXPRESSION

KW - GENE

KW - INITIATION

KW - LIGATION-INDEPENDENT CLONING

KW - ORGANIZATION

KW - PATHWAY

KW - RESPONSE REGULATOR

KW - 1182 Biochemistry, cell and molecular biology

U2 - 10.1016/j.cub.2018.12.041

DO - 10.1016/j.cub.2018.12.041

M3 - Article

VL - 29

SP - 520-+

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 3

ER -