Drug metabolism in droplet scale with on-chip metabolite identification by ambient mass spectrometry

Research output: Conference materialsAbstractResearchpeer-review

Abstract

In this work, we developed and validated a droplet-based drug metabolism screening assay. A digital microfluidic (DMF) approach was adopted to facilitate the actuation of the metabolism reactions in droplet scale. The developed DMF device consists of an integrated low-cost inkjet-printed microheater and a porous polymer based monolith functionalized with immobilized cytochrome P450 (CYP) enzymes. The performance of the droplet-based immobilized enzyme reactors (IMERs) was validated using a fluorescent model reaction detected on-chip using a commercial wellplate reader. For identification of the metabolites, the reaction solution was let dry and the chip was interfaced with mass spectrometry via desorption atmospheric pressure photoionization (DAPPI).
Original languageEnglish
Pages1973-1975
Number of pages3
Publication statusPublished - Nov 2018
MoE publication typeNot Eligible
Event22nd International Conference on Miniaturized
Systems for Chemistry and Life Sciences
- Kaohsiung, Taiwan
Duration: 11 Nov 201815 Nov 2018
Conference number: 22
https://cbmsociety.org/conferences/microtas2018/

Conference

Conference22nd International Conference on Miniaturized
Systems for Chemistry and Life Sciences
Abbreviated titleMicroTAS 2018
CountryTaiwan
CityKaohsiung
Period11/11/201815/11/2018
Internet address

Fields of Science

  • 116 Chemical sciences
  • 317 Pharmacy

Cite this

Sathyanarayanan, G., Haapala, M. J., & Sikanen, T. M. (2018). Drug metabolism in droplet scale with on-chip metabolite identification by ambient mass spectrometry. 1973-1975. Abstract from 22nd International Conference on Miniaturized
Systems for Chemistry and Life Sciences, Kaohsiung, Taiwan.
Sathyanarayanan, Gowtham ; Haapala, Markus Juhani ; Sikanen, Tiina Marjukka. / Drug metabolism in droplet scale with on-chip metabolite identification by ambient mass spectrometry. Abstract from 22nd International Conference on Miniaturized
Systems for Chemistry and Life Sciences, Kaohsiung, Taiwan.3 p.
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title = "Drug metabolism in droplet scale with on-chip metabolite identification by ambient mass spectrometry",
abstract = "In this work, we developed and validated a droplet-based drug metabolism screening assay. A digital microfluidic (DMF) approach was adopted to facilitate the actuation of the metabolism reactions in droplet scale. The developed DMF device consists of an integrated low-cost inkjet-printed microheater and a porous polymer based monolith functionalized with immobilized cytochrome P450 (CYP) enzymes. The performance of the droplet-based immobilized enzyme reactors (IMERs) was validated using a fluorescent model reaction detected on-chip using a commercial wellplate reader. For identification of the metabolites, the reaction solution was let dry and the chip was interfaced with mass spectrometry via desorption atmospheric pressure photoionization (DAPPI).",
keywords = "116 Chemical sciences, 317 Pharmacy",
author = "Gowtham Sathyanarayanan and Haapala, {Markus Juhani} and Sikanen, {Tiina Marjukka}",
year = "2018",
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pages = "1973--1975",
note = "null ; Conference date: 11-11-2018 Through 15-11-2018",
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Drug metabolism in droplet scale with on-chip metabolite identification by ambient mass spectrometry. / Sathyanarayanan, Gowtham; Haapala, Markus Juhani; Sikanen, Tiina Marjukka.

2018. 1973-1975 Abstract from 22nd International Conference on Miniaturized
Systems for Chemistry and Life Sciences, Kaohsiung, Taiwan.

Research output: Conference materialsAbstractResearchpeer-review

TY - CONF

T1 - Drug metabolism in droplet scale with on-chip metabolite identification by ambient mass spectrometry

AU - Sathyanarayanan, Gowtham

AU - Haapala, Markus Juhani

AU - Sikanen, Tiina Marjukka

PY - 2018/11

Y1 - 2018/11

N2 - In this work, we developed and validated a droplet-based drug metabolism screening assay. A digital microfluidic (DMF) approach was adopted to facilitate the actuation of the metabolism reactions in droplet scale. The developed DMF device consists of an integrated low-cost inkjet-printed microheater and a porous polymer based monolith functionalized with immobilized cytochrome P450 (CYP) enzymes. The performance of the droplet-based immobilized enzyme reactors (IMERs) was validated using a fluorescent model reaction detected on-chip using a commercial wellplate reader. For identification of the metabolites, the reaction solution was let dry and the chip was interfaced with mass spectrometry via desorption atmospheric pressure photoionization (DAPPI).

AB - In this work, we developed and validated a droplet-based drug metabolism screening assay. A digital microfluidic (DMF) approach was adopted to facilitate the actuation of the metabolism reactions in droplet scale. The developed DMF device consists of an integrated low-cost inkjet-printed microheater and a porous polymer based monolith functionalized with immobilized cytochrome P450 (CYP) enzymes. The performance of the droplet-based immobilized enzyme reactors (IMERs) was validated using a fluorescent model reaction detected on-chip using a commercial wellplate reader. For identification of the metabolites, the reaction solution was let dry and the chip was interfaced with mass spectrometry via desorption atmospheric pressure photoionization (DAPPI).

KW - 116 Chemical sciences

KW - 317 Pharmacy

M3 - Abstract

SP - 1973

EP - 1975

ER -

Sathyanarayanan G, Haapala MJ, Sikanen TM. Drug metabolism in droplet scale with on-chip metabolite identification by ambient mass spectrometry. 2018. Abstract from 22nd International Conference on Miniaturized
Systems for Chemistry and Life Sciences, Kaohsiung, Taiwan.