Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen Heterobasidion annosum s.l.

Andriy Kovalchuk, Zhen Zeng, Rajendra P. Ghimire, Minna Kivimäenpää, Tommaso Raffaello, Mengxia Liu, Mukrimin Mukrimin, Risto Aarne Olavi Kasanen, Hui Sun, Riitta Julkunen-Tiitto, Jarmo K. Holopainen, Frederick Obioma Asiegbu

Research output: Contribution to journalArticleScientificpeer-review

Abstract

BackgroundRoot and butt rot of conifer trees caused by fungi belonging to the Heterobasidion annosum species complex is one of the most economically important fungal diseases in commercial conifer plantations throughout the Northern hemisphere. We investigated the interactions between Heterobasidion fungi and their host by conducting dual RNA-seq and chemical analysis on Norway spruce trees naturally infected by Heterobasidion spp. We analyzed host and pathogen transcriptome and phenolic and terpenoid contents of the spruce trees.ResultsPresented results emphasize the role of the phenylpropanoid and flavonoid pathways in the chemical defense of Norway spruce trees. Accumulation of lignans was observed in trees displaying symptoms of wood decay. A number of candidate genes with a predicted role in the higher level regulation of spruce defense responses were identified. Our data indicate a possible role of abscisic acid (ABA) signaling in the spruce defense against Heterobasidion infection. Fungal transcripts corresponding to genes encoding carbohydrate- and lignin-degrading enzymes, secondary metabolism genes and effector-like genes were expressed during the host colonization.ConclusionsOur results provide additional insight into defense strategies employed by Norway spruce trees against Heterobasidion infection. The potential applications of the identified candidate genes as markers for higher resistance against root and butt rot deserve further evaluation.

Original languageEnglish
Article number2
JournalBMC Plant Biology
Volume19
Issue number2
Number of pages17
ISSN1471-2229
DOIs
Publication statusPublished - 3 Jan 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • ABSCISIC-ACID
  • CDNA CLONING
  • DEFENSE
  • DIFFERENTIAL EXPRESSION ANALYSIS
  • Dual RNA-seq
  • FUNCTIONAL-CHARACTERIZATION
  • Flavonoids
  • GENE-EXPRESSION
  • Heterobasidion
  • Norway spruce
  • PHENYLPROPANOID METABOLISM
  • PICEA-ABIES
  • Plant defense
  • Root and butt rot
  • TERPENE SYNTHASE
  • TRANSCRIPTION FACTOR
  • Transcriptomics
  • 1183 Plant biology, microbiology, virology

Cite this

@article{c463b4c1906e42caa21ec248b648d859,
title = "Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen Heterobasidion annosum s.l.",
abstract = "BackgroundRoot and butt rot of conifer trees caused by fungi belonging to the Heterobasidion annosum species complex is one of the most economically important fungal diseases in commercial conifer plantations throughout the Northern hemisphere. We investigated the interactions between Heterobasidion fungi and their host by conducting dual RNA-seq and chemical analysis on Norway spruce trees naturally infected by Heterobasidion spp. We analyzed host and pathogen transcriptome and phenolic and terpenoid contents of the spruce trees.ResultsPresented results emphasize the role of the phenylpropanoid and flavonoid pathways in the chemical defense of Norway spruce trees. Accumulation of lignans was observed in trees displaying symptoms of wood decay. A number of candidate genes with a predicted role in the higher level regulation of spruce defense responses were identified. Our data indicate a possible role of abscisic acid (ABA) signaling in the spruce defense against Heterobasidion infection. Fungal transcripts corresponding to genes encoding carbohydrate- and lignin-degrading enzymes, secondary metabolism genes and effector-like genes were expressed during the host colonization.ConclusionsOur results provide additional insight into defense strategies employed by Norway spruce trees against Heterobasidion infection. The potential applications of the identified candidate genes as markers for higher resistance against root and butt rot deserve further evaluation.",
keywords = "ABSCISIC-ACID, CDNA CLONING, DEFENSE, DIFFERENTIAL EXPRESSION ANALYSIS, Dual RNA-seq, FUNCTIONAL-CHARACTERIZATION, Flavonoids, GENE-EXPRESSION, Heterobasidion, Norway spruce, PHENYLPROPANOID METABOLISM, PICEA-ABIES, Plant defense, Root and butt rot, TERPENE SYNTHASE, TRANSCRIPTION FACTOR, Transcriptomics, 1183 Plant biology, microbiology, virology",
author = "Andriy Kovalchuk and Zhen Zeng and Ghimire, {Rajendra P.} and Minna Kivim{\"a}enp{\"a}{\"a} and Tommaso Raffaello and Mengxia Liu and Mukrimin Mukrimin and Kasanen, {Risto Aarne Olavi} and Hui Sun and Riitta Julkunen-Tiitto and Holopainen, {Jarmo K.} and Asiegbu, {Frederick Obioma}",
year = "2019",
month = "1",
day = "3",
doi = "10.1186/s12870-018-1602-0",
language = "English",
volume = "19",
journal = "BMC Plant Biology",
issn = "1471-2229",
publisher = "BMC",
number = "2",

}

Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen Heterobasidion annosum s.l. / Kovalchuk, Andriy; Zeng, Zhen; Ghimire, Rajendra P.; Kivimäenpää, Minna; Raffaello, Tommaso; Liu, Mengxia; Mukrimin, Mukrimin; Kasanen, Risto Aarne Olavi; Sun, Hui; Julkunen-Tiitto, Riitta; Holopainen, Jarmo K.; Asiegbu, Frederick Obioma.

In: BMC Plant Biology, Vol. 19, No. 2, 2, 03.01.2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen Heterobasidion annosum s.l.

AU - Kovalchuk, Andriy

AU - Zeng, Zhen

AU - Ghimire, Rajendra P.

AU - Kivimäenpää, Minna

AU - Raffaello, Tommaso

AU - Liu, Mengxia

AU - Mukrimin, Mukrimin

AU - Kasanen, Risto Aarne Olavi

AU - Sun, Hui

AU - Julkunen-Tiitto, Riitta

AU - Holopainen, Jarmo K.

AU - Asiegbu, Frederick Obioma

PY - 2019/1/3

Y1 - 2019/1/3

N2 - BackgroundRoot and butt rot of conifer trees caused by fungi belonging to the Heterobasidion annosum species complex is one of the most economically important fungal diseases in commercial conifer plantations throughout the Northern hemisphere. We investigated the interactions between Heterobasidion fungi and their host by conducting dual RNA-seq and chemical analysis on Norway spruce trees naturally infected by Heterobasidion spp. We analyzed host and pathogen transcriptome and phenolic and terpenoid contents of the spruce trees.ResultsPresented results emphasize the role of the phenylpropanoid and flavonoid pathways in the chemical defense of Norway spruce trees. Accumulation of lignans was observed in trees displaying symptoms of wood decay. A number of candidate genes with a predicted role in the higher level regulation of spruce defense responses were identified. Our data indicate a possible role of abscisic acid (ABA) signaling in the spruce defense against Heterobasidion infection. Fungal transcripts corresponding to genes encoding carbohydrate- and lignin-degrading enzymes, secondary metabolism genes and effector-like genes were expressed during the host colonization.ConclusionsOur results provide additional insight into defense strategies employed by Norway spruce trees against Heterobasidion infection. The potential applications of the identified candidate genes as markers for higher resistance against root and butt rot deserve further evaluation.

AB - BackgroundRoot and butt rot of conifer trees caused by fungi belonging to the Heterobasidion annosum species complex is one of the most economically important fungal diseases in commercial conifer plantations throughout the Northern hemisphere. We investigated the interactions between Heterobasidion fungi and their host by conducting dual RNA-seq and chemical analysis on Norway spruce trees naturally infected by Heterobasidion spp. We analyzed host and pathogen transcriptome and phenolic and terpenoid contents of the spruce trees.ResultsPresented results emphasize the role of the phenylpropanoid and flavonoid pathways in the chemical defense of Norway spruce trees. Accumulation of lignans was observed in trees displaying symptoms of wood decay. A number of candidate genes with a predicted role in the higher level regulation of spruce defense responses were identified. Our data indicate a possible role of abscisic acid (ABA) signaling in the spruce defense against Heterobasidion infection. Fungal transcripts corresponding to genes encoding carbohydrate- and lignin-degrading enzymes, secondary metabolism genes and effector-like genes were expressed during the host colonization.ConclusionsOur results provide additional insight into defense strategies employed by Norway spruce trees against Heterobasidion infection. The potential applications of the identified candidate genes as markers for higher resistance against root and butt rot deserve further evaluation.

KW - ABSCISIC-ACID

KW - CDNA CLONING

KW - DEFENSE

KW - DIFFERENTIAL EXPRESSION ANALYSIS

KW - Dual RNA-seq

KW - FUNCTIONAL-CHARACTERIZATION

KW - Flavonoids

KW - GENE-EXPRESSION

KW - Heterobasidion

KW - Norway spruce

KW - PHENYLPROPANOID METABOLISM

KW - PICEA-ABIES

KW - Plant defense

KW - Root and butt rot

KW - TERPENE SYNTHASE

KW - TRANSCRIPTION FACTOR

KW - Transcriptomics

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1186/s12870-018-1602-0

DO - 10.1186/s12870-018-1602-0

M3 - Article

VL - 19

JO - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

IS - 2

M1 - 2

ER -