Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer

C L Andersen, L L Christensen, K Thorsen, T Schepeler, F B Sorensen, H W Verspaget, R Simon, M Kruhoffer, L A Aaltonen, S Laurberg, T F Ørntoft

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    "The aim of this study was to identify deregulated transcription factors (TFs) in colorectal cancer (CRC) and to evaluate their relation with the recurrence of stage II CRC and overall survival. Microarray-based transcript profiles of 20 normal mucosas and 424 CRC samples were used to identify 51 TFs displaying differential transcript levels between normal mucosa and CRC. For a subset of these we provide in vitro evidence that deregulation of the Wnt signalling pathway can lead to the alterations observed in tissues. Furthermore, in two independent cohorts of microsatellite-stable stage II cancers we found that high SOX4 transcript levels correlated with recurrence (HR 2.7; 95% CI, 1.2-6.0; P =0.01). Analyses of similar to 1000 stage I-III adenocarcinomas, by immunohistochemistry, revealed that patients with tumours displaying high levels of CBFB and SMARCC1 proteins had a significantly better overall survival rate ( P =0.0001 and P =0.0275, respectively) than patients with low levels. Multivariate analyses revealed that a high CBFB protein level was an independent predictor of survival. In conclusion, several of the identified TFs seem to be involved in the progression of CRC."
    Original languageEnglish
    JournalBritish Journal of Cancer
    Volume100
    Issue number3
    Pages (from-to)511-523
    Number of pages13
    ISSN0007-0920
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • 311 Basic medicine

    Cite this

    Andersen, C. L., Christensen, L. L., Thorsen, K., Schepeler, T., Sorensen, F. B., Verspaget, H. W., ... Ørntoft, T. F. (2009). Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer. British Journal of Cancer, 100(3), 511-523. https://doi.org/10.1038/sj.bjc.6604884
    Andersen, C L ; Christensen, L L ; Thorsen, K ; Schepeler, T ; Sorensen, F B ; Verspaget, H W ; Simon, R ; Kruhoffer, M ; Aaltonen, L A ; Laurberg, S ; Ørntoft, T F. / Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer. In: British Journal of Cancer. 2009 ; Vol. 100, No. 3. pp. 511-523.
    @article{0f689b7192c14a0392c87e144d829ed2,
    title = "Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer",
    abstract = "{"}The aim of this study was to identify deregulated transcription factors (TFs) in colorectal cancer (CRC) and to evaluate their relation with the recurrence of stage II CRC and overall survival. Microarray-based transcript profiles of 20 normal mucosas and 424 CRC samples were used to identify 51 TFs displaying differential transcript levels between normal mucosa and CRC. For a subset of these we provide in vitro evidence that deregulation of the Wnt signalling pathway can lead to the alterations observed in tissues. Furthermore, in two independent cohorts of microsatellite-stable stage II cancers we found that high SOX4 transcript levels correlated with recurrence (HR 2.7; 95{\%} CI, 1.2-6.0; P =0.01). Analyses of similar to 1000 stage I-III adenocarcinomas, by immunohistochemistry, revealed that patients with tumours displaying high levels of CBFB and SMARCC1 proteins had a significantly better overall survival rate ( P =0.0001 and P =0.0275, respectively) than patients with low levels. Multivariate analyses revealed that a high CBFB protein level was an independent predictor of survival. In conclusion, several of the identified TFs seem to be involved in the progression of CRC.{"}",
    keywords = "311 Basic medicine",
    author = "Andersen, {C L} and Christensen, {L L} and K Thorsen and T Schepeler and Sorensen, {F B} and Verspaget, {H W} and R Simon and M Kruhoffer and Aaltonen, {L A} and S Laurberg and {\O}rntoft, {T F}",
    year = "2009",
    doi = "10.1038/sj.bjc.6604884",
    language = "English",
    volume = "100",
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    Andersen, CL, Christensen, LL, Thorsen, K, Schepeler, T, Sorensen, FB, Verspaget, HW, Simon, R, Kruhoffer, M, Aaltonen, LA, Laurberg, S & Ørntoft, TF 2009, 'Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer', British Journal of Cancer, vol. 100, no. 3, pp. 511-523. https://doi.org/10.1038/sj.bjc.6604884

    Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer. / Andersen, C L; Christensen, L L; Thorsen, K; Schepeler, T; Sorensen, F B; Verspaget, H W; Simon, R; Kruhoffer, M; Aaltonen, L A; Laurberg, S; Ørntoft, T F.

    In: British Journal of Cancer, Vol. 100, No. 3, 2009, p. 511-523.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer

    AU - Andersen, C L

    AU - Christensen, L L

    AU - Thorsen, K

    AU - Schepeler, T

    AU - Sorensen, F B

    AU - Verspaget, H W

    AU - Simon, R

    AU - Kruhoffer, M

    AU - Aaltonen, L A

    AU - Laurberg, S

    AU - Ørntoft, T F

    PY - 2009

    Y1 - 2009

    N2 - "The aim of this study was to identify deregulated transcription factors (TFs) in colorectal cancer (CRC) and to evaluate their relation with the recurrence of stage II CRC and overall survival. Microarray-based transcript profiles of 20 normal mucosas and 424 CRC samples were used to identify 51 TFs displaying differential transcript levels between normal mucosa and CRC. For a subset of these we provide in vitro evidence that deregulation of the Wnt signalling pathway can lead to the alterations observed in tissues. Furthermore, in two independent cohorts of microsatellite-stable stage II cancers we found that high SOX4 transcript levels correlated with recurrence (HR 2.7; 95% CI, 1.2-6.0; P =0.01). Analyses of similar to 1000 stage I-III adenocarcinomas, by immunohistochemistry, revealed that patients with tumours displaying high levels of CBFB and SMARCC1 proteins had a significantly better overall survival rate ( P =0.0001 and P =0.0275, respectively) than patients with low levels. Multivariate analyses revealed that a high CBFB protein level was an independent predictor of survival. In conclusion, several of the identified TFs seem to be involved in the progression of CRC."

    AB - "The aim of this study was to identify deregulated transcription factors (TFs) in colorectal cancer (CRC) and to evaluate their relation with the recurrence of stage II CRC and overall survival. Microarray-based transcript profiles of 20 normal mucosas and 424 CRC samples were used to identify 51 TFs displaying differential transcript levels between normal mucosa and CRC. For a subset of these we provide in vitro evidence that deregulation of the Wnt signalling pathway can lead to the alterations observed in tissues. Furthermore, in two independent cohorts of microsatellite-stable stage II cancers we found that high SOX4 transcript levels correlated with recurrence (HR 2.7; 95% CI, 1.2-6.0; P =0.01). Analyses of similar to 1000 stage I-III adenocarcinomas, by immunohistochemistry, revealed that patients with tumours displaying high levels of CBFB and SMARCC1 proteins had a significantly better overall survival rate ( P =0.0001 and P =0.0275, respectively) than patients with low levels. Multivariate analyses revealed that a high CBFB protein level was an independent predictor of survival. In conclusion, several of the identified TFs seem to be involved in the progression of CRC."

    KW - 311 Basic medicine

    U2 - 10.1038/sj.bjc.6604884

    DO - 10.1038/sj.bjc.6604884

    M3 - Article

    VL - 100

    SP - 511

    EP - 523

    JO - British Journal of Cancer

    JF - British Journal of Cancer

    SN - 0007-0920

    IS - 3

    ER -