Effect of chronic nicotine treatment on localization of neuronal nicotinic acetylcholine receptors at cellular level

Jukka S Pakkanen, Jan Stenfors, Eija Jokitalo, Raimo K Tuominen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Chronic nicotine treatment increases the number of neuronal nicotinic acetylcholine receptors (nAChRs). Localization of nAChRs at a cellular level determines their functional role. However, changes in the localization of nAChRs caused by chronic nicotine treatment are not well known. In this study, we have examined the effects of chronic nicotine treatment on alpha 7 and beta 2 nAChR subunits in vitro in cell lines and in vivo in mouse striatum. In vitro, two different cell lines were used, SH-SY5Y cells endogenously expressing several nAChR subtypes and SH-EP1-h alpha 7 cells, transfected with the human alpha 7 nAChR subunit gene. Effects of chronic nicotine treatment (10 mu M, 3 days) were studied in vitro by using confocal and electron microscopy and calcium fluorometry. In vitro in SH-SY5Y cells, alpha 7 and beta 2 subunits formed groups, unlike alpha 7 subunits in SH-EP1-ha7 cells, which were partially localized on endoplastic reticulum. Chronic nicotine treatment did not change the localization of nAChRs in endosomes, but caused clustering of alpha 7 subunits in SH-EP1-h alpha 7 cells. In vivo, nicotine was given to mice in their drinking water for 7 weeks. Results showed that alpha 7 and beta 2 subunits formed groups, and that chronic nicotine treatment increased the size of the clusters. As a conclusion, our data show that there are large intracellular pools of nAChR subunits, which are partially localized on endoplastic reticulum. Chronic nicotine treatment does not change endocytotic trafficking of nAChRs. Chronic nicotine treatment increased clustering of nAChRs, which could have a role in the release of dopamine (DA) evoked by nicotine.
Original languageEnglish
JournalSynapse (New York)
Volume59
Issue number7
Pages (from-to)383-393
Number of pages11
ISSN0887-4476
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 317 Pharmacy

Cite this

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title = "Effect of chronic nicotine treatment on localization of neuronal nicotinic acetylcholine receptors at cellular level",
abstract = "Chronic nicotine treatment increases the number of neuronal nicotinic acetylcholine receptors (nAChRs). Localization of nAChRs at a cellular level determines their functional role. However, changes in the localization of nAChRs caused by chronic nicotine treatment are not well known. In this study, we have examined the effects of chronic nicotine treatment on alpha 7 and beta 2 nAChR subunits in vitro in cell lines and in vivo in mouse striatum. In vitro, two different cell lines were used, SH-SY5Y cells endogenously expressing several nAChR subtypes and SH-EP1-h alpha 7 cells, transfected with the human alpha 7 nAChR subunit gene. Effects of chronic nicotine treatment (10 mu M, 3 days) were studied in vitro by using confocal and electron microscopy and calcium fluorometry. In vitro in SH-SY5Y cells, alpha 7 and beta 2 subunits formed groups, unlike alpha 7 subunits in SH-EP1-ha7 cells, which were partially localized on endoplastic reticulum. Chronic nicotine treatment did not change the localization of nAChRs in endosomes, but caused clustering of alpha 7 subunits in SH-EP1-h alpha 7 cells. In vivo, nicotine was given to mice in their drinking water for 7 weeks. Results showed that alpha 7 and beta 2 subunits formed groups, and that chronic nicotine treatment increased the size of the clusters. As a conclusion, our data show that there are large intracellular pools of nAChR subunits, which are partially localized on endoplastic reticulum. Chronic nicotine treatment does not change endocytotic trafficking of nAChRs. Chronic nicotine treatment increased clustering of nAChRs, which could have a role in the release of dopamine (DA) evoked by nicotine.",
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author = "Pakkanen, {Jukka S} and Jan Stenfors and Eija Jokitalo and Tuominen, {Raimo K}",
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Effect of chronic nicotine treatment on localization of neuronal nicotinic acetylcholine receptors at cellular level. / Pakkanen, Jukka S; Stenfors, Jan; Jokitalo, Eija; Tuominen, Raimo K.

In: Synapse (New York), Vol. 59, No. 7, 2006, p. 383-393.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of chronic nicotine treatment on localization of neuronal nicotinic acetylcholine receptors at cellular level

AU - Pakkanen, Jukka S

AU - Stenfors, Jan

AU - Jokitalo, Eija

AU - Tuominen, Raimo K

PY - 2006

Y1 - 2006

N2 - Chronic nicotine treatment increases the number of neuronal nicotinic acetylcholine receptors (nAChRs). Localization of nAChRs at a cellular level determines their functional role. However, changes in the localization of nAChRs caused by chronic nicotine treatment are not well known. In this study, we have examined the effects of chronic nicotine treatment on alpha 7 and beta 2 nAChR subunits in vitro in cell lines and in vivo in mouse striatum. In vitro, two different cell lines were used, SH-SY5Y cells endogenously expressing several nAChR subtypes and SH-EP1-h alpha 7 cells, transfected with the human alpha 7 nAChR subunit gene. Effects of chronic nicotine treatment (10 mu M, 3 days) were studied in vitro by using confocal and electron microscopy and calcium fluorometry. In vitro in SH-SY5Y cells, alpha 7 and beta 2 subunits formed groups, unlike alpha 7 subunits in SH-EP1-ha7 cells, which were partially localized on endoplastic reticulum. Chronic nicotine treatment did not change the localization of nAChRs in endosomes, but caused clustering of alpha 7 subunits in SH-EP1-h alpha 7 cells. In vivo, nicotine was given to mice in their drinking water for 7 weeks. Results showed that alpha 7 and beta 2 subunits formed groups, and that chronic nicotine treatment increased the size of the clusters. As a conclusion, our data show that there are large intracellular pools of nAChR subunits, which are partially localized on endoplastic reticulum. Chronic nicotine treatment does not change endocytotic trafficking of nAChRs. Chronic nicotine treatment increased clustering of nAChRs, which could have a role in the release of dopamine (DA) evoked by nicotine.

AB - Chronic nicotine treatment increases the number of neuronal nicotinic acetylcholine receptors (nAChRs). Localization of nAChRs at a cellular level determines their functional role. However, changes in the localization of nAChRs caused by chronic nicotine treatment are not well known. In this study, we have examined the effects of chronic nicotine treatment on alpha 7 and beta 2 nAChR subunits in vitro in cell lines and in vivo in mouse striatum. In vitro, two different cell lines were used, SH-SY5Y cells endogenously expressing several nAChR subtypes and SH-EP1-h alpha 7 cells, transfected with the human alpha 7 nAChR subunit gene. Effects of chronic nicotine treatment (10 mu M, 3 days) were studied in vitro by using confocal and electron microscopy and calcium fluorometry. In vitro in SH-SY5Y cells, alpha 7 and beta 2 subunits formed groups, unlike alpha 7 subunits in SH-EP1-ha7 cells, which were partially localized on endoplastic reticulum. Chronic nicotine treatment did not change the localization of nAChRs in endosomes, but caused clustering of alpha 7 subunits in SH-EP1-h alpha 7 cells. In vivo, nicotine was given to mice in their drinking water for 7 weeks. Results showed that alpha 7 and beta 2 subunits formed groups, and that chronic nicotine treatment increased the size of the clusters. As a conclusion, our data show that there are large intracellular pools of nAChR subunits, which are partially localized on endoplastic reticulum. Chronic nicotine treatment does not change endocytotic trafficking of nAChRs. Chronic nicotine treatment increased clustering of nAChRs, which could have a role in the release of dopamine (DA) evoked by nicotine.

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DO - 10.1002/syn.20249

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SP - 383

EP - 393

JO - Synapse (New York)

JF - Synapse (New York)

SN - 0887-4476

IS - 7

ER -