Effect of copper, nutrient nitrogen, and wood-supplement on the production of lignin-modifying enzymes by the white-rot fungus Phlebia radiata

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Production of the oxidoreductive lignin-modifying enzymes – lignin and manganese peroxidases (MnPs), and laccase – of the white-rot basidiomycete Phlebia radiata was investigated in semi-solid cultures supplemented with milled grey alder or Norway spruce and charcoal. Concentrations of nutrient nitrogen and Cu-supplement varied also in the cultures. According to extracellular activities, production of both lignin peroxidase (LiP) and MnP was significantly promoted with wood as carbon source, with milled alder (MA) and low nitrogen (LN) resulting with the maximal LiP activities (550 nkat l−1) and noticeable levels of MnP (3 μkat l−1). Activities of LiP and MnP were also elevated on high nitrogen (HN) complex medium when supplemented with spruce and charcoal. Maximal laccase activities (22 and 29 μkat l−1) were obtained in extra high nitrogen (eHN) containing defined and complex media supplemented with 1.5 mM Cu2+. However, the nitrogen source, either peptone or ammonium nitrate and asparagine, caused no stimulation on laccase production without Cu-supplement. This is also the first report to demonstrate a new, on high Cu2+ amended medium produced extracellular laccase of P. radiata with pI value of 4.9, thereby complementing our previous findings on gene expression, and cloning of a second laccase of this fungus.
Original languageEnglish
JournalFungal Biology
Volume117
Issue number1
Pages (from-to)62-70
Number of pages9
ISSN1878-6146
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 219 Environmental biotechnology
  • Enzyme production
  • 414 Agricultural biotechnology
  • Wood-decaying fungi
  • Lignin biodegradation
  • 1183 Plant biology, microbiology, virology
  • Fungal biology
  • Nutrient copper

Cite this

@article{f2fc9afb327e4ba8b72c97c7b2819be2,
title = "Effect of copper, nutrient nitrogen, and wood-supplement on the production of lignin-modifying enzymes by the white-rot fungus Phlebia radiata",
abstract = "Production of the oxidoreductive lignin-modifying enzymes – lignin and manganese peroxidases (MnPs), and laccase – of the white-rot basidiomycete Phlebia radiata was investigated in semi-solid cultures supplemented with milled grey alder or Norway spruce and charcoal. Concentrations of nutrient nitrogen and Cu-supplement varied also in the cultures. According to extracellular activities, production of both lignin peroxidase (LiP) and MnP was significantly promoted with wood as carbon source, with milled alder (MA) and low nitrogen (LN) resulting with the maximal LiP activities (550 nkat l−1) and noticeable levels of MnP (3 μkat l−1). Activities of LiP and MnP were also elevated on high nitrogen (HN) complex medium when supplemented with spruce and charcoal. Maximal laccase activities (22 and 29 μkat l−1) were obtained in extra high nitrogen (eHN) containing defined and complex media supplemented with 1.5 mM Cu2+. However, the nitrogen source, either peptone or ammonium nitrate and asparagine, caused no stimulation on laccase production without Cu-supplement. This is also the first report to demonstrate a new, on high Cu2+ amended medium produced extracellular laccase of P. radiata with pI value of 4.9, thereby complementing our previous findings on gene expression, and cloning of a second laccase of this fungus.",
keywords = "219 Environmental biotechnology, Enzyme production, 414 Agricultural biotechnology, Wood-decaying fungi, Lignin biodegradation, 1183 Plant biology, microbiology, virology, Fungal biology, Nutrient copper",
author = "Miia M{\"a}kel{\"a} and Taina Lundell and Annele Hatakka and Kristiina Hilden",
year = "2013",
doi = "10.1016/j.funbio.2012.11.006",
language = "English",
volume = "117",
pages = "62--70",
journal = "Fungal Biology",
issn = "1878-6146",
publisher = "ELSEVIER SCI IRELAND LTD",
number = "1",

}

TY - JOUR

T1 - Effect of copper, nutrient nitrogen, and wood-supplement on the production of lignin-modifying enzymes by the white-rot fungus Phlebia radiata

AU - Mäkelä, Miia

AU - Lundell, Taina

AU - Hatakka, Annele

AU - Hilden, Kristiina

PY - 2013

Y1 - 2013

N2 - Production of the oxidoreductive lignin-modifying enzymes – lignin and manganese peroxidases (MnPs), and laccase – of the white-rot basidiomycete Phlebia radiata was investigated in semi-solid cultures supplemented with milled grey alder or Norway spruce and charcoal. Concentrations of nutrient nitrogen and Cu-supplement varied also in the cultures. According to extracellular activities, production of both lignin peroxidase (LiP) and MnP was significantly promoted with wood as carbon source, with milled alder (MA) and low nitrogen (LN) resulting with the maximal LiP activities (550 nkat l−1) and noticeable levels of MnP (3 μkat l−1). Activities of LiP and MnP were also elevated on high nitrogen (HN) complex medium when supplemented with spruce and charcoal. Maximal laccase activities (22 and 29 μkat l−1) were obtained in extra high nitrogen (eHN) containing defined and complex media supplemented with 1.5 mM Cu2+. However, the nitrogen source, either peptone or ammonium nitrate and asparagine, caused no stimulation on laccase production without Cu-supplement. This is also the first report to demonstrate a new, on high Cu2+ amended medium produced extracellular laccase of P. radiata with pI value of 4.9, thereby complementing our previous findings on gene expression, and cloning of a second laccase of this fungus.

AB - Production of the oxidoreductive lignin-modifying enzymes – lignin and manganese peroxidases (MnPs), and laccase – of the white-rot basidiomycete Phlebia radiata was investigated in semi-solid cultures supplemented with milled grey alder or Norway spruce and charcoal. Concentrations of nutrient nitrogen and Cu-supplement varied also in the cultures. According to extracellular activities, production of both lignin peroxidase (LiP) and MnP was significantly promoted with wood as carbon source, with milled alder (MA) and low nitrogen (LN) resulting with the maximal LiP activities (550 nkat l−1) and noticeable levels of MnP (3 μkat l−1). Activities of LiP and MnP were also elevated on high nitrogen (HN) complex medium when supplemented with spruce and charcoal. Maximal laccase activities (22 and 29 μkat l−1) were obtained in extra high nitrogen (eHN) containing defined and complex media supplemented with 1.5 mM Cu2+. However, the nitrogen source, either peptone or ammonium nitrate and asparagine, caused no stimulation on laccase production without Cu-supplement. This is also the first report to demonstrate a new, on high Cu2+ amended medium produced extracellular laccase of P. radiata with pI value of 4.9, thereby complementing our previous findings on gene expression, and cloning of a second laccase of this fungus.

KW - 219 Environmental biotechnology

KW - Enzyme production

KW - 414 Agricultural biotechnology

KW - Wood-decaying fungi

KW - Lignin biodegradation

KW - 1183 Plant biology, microbiology, virology

KW - Fungal biology

KW - Nutrient copper

U2 - 10.1016/j.funbio.2012.11.006

DO - 10.1016/j.funbio.2012.11.006

M3 - Article

VL - 117

SP - 62

EP - 70

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

IS - 1

ER -