Effect of eluent on the ionization process in liquid chromatography-mass spectrometry

    Research output: Contribution to journalReview ArticleScientificpeer-review

    Abstract

    The most widely used ionization techniques in liquid chromatography-mass spectrometry (LC-MS) are electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). All three provide user friendly Coupling of LC to MS. Achieving optimal LC-MS conditions is not always easy, however, owing to the complexity of ionization processes and the many parameters affecting mass spectrometric sensitivity and chromatographic performance. The selection of eluent composition requires particular attention since a solvent that is optimal for analyte ionization often does not Provide acceptable retention and resolution in LC. Compromises must then be made between ionization and chromatographic separation efficiencies. The review presents an overview of studies concerning the effect of eluent composition on the ionization efficiency of ESI, APCI and APPI in LC-MS. Solvent characteristics are discussed in the light of ionization theories, and selected analytical applications are described. The aim is to provide practical background information for the development and optimization of LC-MS methods. (c) 2008 Elsevier B.V. All rights reserved
    Original languageEnglish
    JournalJournal of Chromatography. A
    Volume1216
    Issue number4
    Pages (from-to)685-699
    Number of pages15
    ISSN0021-9673
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA2 Review article in a scientific journal

    Cite this

    @article{2ef850fb8933435585a1fc85b7712078,
    title = "Effect of eluent on the ionization process in liquid chromatography-mass spectrometry",
    abstract = "The most widely used ionization techniques in liquid chromatography-mass spectrometry (LC-MS) are electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). All three provide user friendly Coupling of LC to MS. Achieving optimal LC-MS conditions is not always easy, however, owing to the complexity of ionization processes and the many parameters affecting mass spectrometric sensitivity and chromatographic performance. The selection of eluent composition requires particular attention since a solvent that is optimal for analyte ionization often does not Provide acceptable retention and resolution in LC. Compromises must then be made between ionization and chromatographic separation efficiencies. The review presents an overview of studies concerning the effect of eluent composition on the ionization efficiency of ESI, APCI and APPI in LC-MS. Solvent characteristics are discussed in the light of ionization theories, and selected analytical applications are described. The aim is to provide practical background information for the development and optimization of LC-MS methods. (c) 2008 Elsevier B.V. All rights reserved",
    author = "Risto Kostiainen and Tiina Kauppila",
    year = "2009",
    doi = "10.1016/j.chroma.2008.08.095",
    language = "English",
    volume = "1216",
    pages = "685--699",
    journal = "Journal of Chromatography. A",
    issn = "0021-9673",
    publisher = "Elsevier Scientific Publ. Co",
    number = "4",

    }

    Effect of eluent on the ionization process in liquid chromatography-mass spectrometry. / Kostiainen, Risto; Kauppila, Tiina.

    In: Journal of Chromatography. A, Vol. 1216, No. 4, 2009, p. 685-699.

    Research output: Contribution to journalReview ArticleScientificpeer-review

    TY - JOUR

    T1 - Effect of eluent on the ionization process in liquid chromatography-mass spectrometry

    AU - Kostiainen, Risto

    AU - Kauppila, Tiina

    PY - 2009

    Y1 - 2009

    N2 - The most widely used ionization techniques in liquid chromatography-mass spectrometry (LC-MS) are electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). All three provide user friendly Coupling of LC to MS. Achieving optimal LC-MS conditions is not always easy, however, owing to the complexity of ionization processes and the many parameters affecting mass spectrometric sensitivity and chromatographic performance. The selection of eluent composition requires particular attention since a solvent that is optimal for analyte ionization often does not Provide acceptable retention and resolution in LC. Compromises must then be made between ionization and chromatographic separation efficiencies. The review presents an overview of studies concerning the effect of eluent composition on the ionization efficiency of ESI, APCI and APPI in LC-MS. Solvent characteristics are discussed in the light of ionization theories, and selected analytical applications are described. The aim is to provide practical background information for the development and optimization of LC-MS methods. (c) 2008 Elsevier B.V. All rights reserved

    AB - The most widely used ionization techniques in liquid chromatography-mass spectrometry (LC-MS) are electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). All three provide user friendly Coupling of LC to MS. Achieving optimal LC-MS conditions is not always easy, however, owing to the complexity of ionization processes and the many parameters affecting mass spectrometric sensitivity and chromatographic performance. The selection of eluent composition requires particular attention since a solvent that is optimal for analyte ionization often does not Provide acceptable retention and resolution in LC. Compromises must then be made between ionization and chromatographic separation efficiencies. The review presents an overview of studies concerning the effect of eluent composition on the ionization efficiency of ESI, APCI and APPI in LC-MS. Solvent characteristics are discussed in the light of ionization theories, and selected analytical applications are described. The aim is to provide practical background information for the development and optimization of LC-MS methods. (c) 2008 Elsevier B.V. All rights reserved

    U2 - 10.1016/j.chroma.2008.08.095

    DO - 10.1016/j.chroma.2008.08.095

    M3 - Review Article

    VL - 1216

    SP - 685

    EP - 699

    JO - Journal of Chromatography. A

    JF - Journal of Chromatography. A

    SN - 0021-9673

    IS - 4

    ER -