Effect of temporin A modifications on its cytotoxicity and antimicrobial activity

T Mäntylä, H Sirola, E Kansanen, Timo Korjamo, Hilkka Lankinen, K Lappalainen, A. L Välimaa, Ilkka Harvima, Ale Närvänen

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    Temporin A (TA), a short a-helical antimicrobial peptide isolated from the skin of the frog Rana temporaria, is effective against a broad spectrum of Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium strains. TA interacts directly with the cell membrane of the microorganism and it has been reported to be non-toxic to erythrocytes at concentrations that are antimicrobial. Less is known about the effects on the viability and growth of nucleated eukaryotic cells. In this study we have tested antibacterial and growth-inhibitory properties of TA, its dimeric analogue (TAd), and all-L (TAL L512) and all-D (TAD L512) enantiomeric derivatives of modified TA towards S. aureus and cultured human keratinocytes, respectively. All molecules were antibacterial at concentrations from 1.5 mu M to 10 mu M. In keratinocyte cultures, TAD L512, as well as TAd, showed cytotoxicity. The original TA and TAL L512 did not affect the viability of the cells at their bacteriolytic concentrations. The growth of keratinocytes in low- and high-calcium media was only slightly inhibited by temporins at concentrations which were antibacterial to S. aureus. This suggests that original TA and its modification, TAL L512, are promising molecules against multiresistant bacterial infections.
    Original languageEnglish
    JournalAPMIS : Acta pathologica, microbiologica et immunologica Scandinavica
    Volume113
    Issue number7/8
    Pages (from-to)497-505
    Number of pages9
    ISSN0903-4641
    Publication statusPublished - 2005
    MoE publication typeA1 Journal article-refereed

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