Effects of energy intake on lipidomic profile of liver and adipose tissue during the periparturient period in dairy cows

Tuomo Kokkonen, Nanbing Qin, Mika Hilvo, Siru Salin, Shaimaa Selim, Tuulikki Seppänen-Laakso, Juhani Taponen, Aila Vanhatalo, Kari Elo

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Abstract

Voluntary dry matter intake (DMI) of dairy cows greatly exceeds energy requirements during dry period (Loor et al 2006). Typically, DMI decreases during the last weeks of pregnancy and this is accompanied by a gradual increase of lipid mobilization from adipose tissue (Ingvartsen and Andersen 2000; Grummer et al 2004). High energy intake may change the lipid profile in peripheral tissues of cows and subsequently influence lipid mobilization and gluconeogenesis through the regulation of insulin sensitivity during transition from pregnancy to lactation. Our aim was to study the effects of high and restricted energy allowances on lipidomic profiles of the liver and adipose tissue in dairy cows during the periparturient period. The study was conducted with 16 multiparous cows in a randomized complete block design. The treatments consisted of controlled-energy feeding (100% of energy requirement of pregnant dairy cows, CON) and high-energy feeding (142% of energy requirement of pregnant dairy cows, HIGH). In HIGH group, decreasing feed allowance was implemented during the last three weeks of pregnancy to study the combined effect of high energy intake during the early dry period and the diminishing feed intake with approaching calving. Samples of subcutaneous adipose tissue and liver were collected by biopsy at 10 days prepartum, and 1 day and 9 days postpartum. Lipids were identified into classes including ceramide (Cer), triglyceride (TG), diacylglycerol (DG), lysophospholipid (Lyso), phosphatidylcholines (PC), phosphatidyl ethanolamine (PE), phosphatidylinositol (PI), and sphingomyelin (SM), and quantified using liquid chromatography-mass spectrometry based lipidomics. The statistical analysis was conducted using a mixed model that included the random effect of block and fixed effects of treatment, sampling day and their interaction. In the liver, concentration of Cer, Lyso, PC, PE, and SM was higher in HIGH group (P<0.05) compared to CON group during the periparturient period (Table 1). The concentration of TG and DG increased dramatically after parturition (P<0.001) in both groups, while the concentration of SM decreased. No statistically significant interactions between diet and time were observed. In the adipose tissue, overfeeding of energy tended to (P<0.10) increase concentration of Cer and decrease SM during the periparturient period. The level of each lipid class in adipose tissue displayed no significant tendency across the time points (P>0.05). Interactions between diet and time were observed in PC (P<0.05), and a tendency towards interaction in Cer, PE, and PI (P<0.10). The concentrations of these lipids decreased in HIGH group, and increased in CON group during the periparturient period. In conclusion, we have observed significant differences in lipid classes which indicate that energy intake may have affected insulin sensitivity of subcutaneous adipose tissue and liver during the periparturient period.
Original languageEnglish
Title of host publication Harnessing the Ecology and Physiology of Herbivores : Proceedings of the 30th Biennial Conference of the Australian Society of Animal Production
EditorsS. Hatcher, G. L. Krebs, B. V. B. Holman
Number of pages1
Place of PublicationCamberra
PublisherAustralian Society of Animal Production
Publication date2014
Pages271
Publication statusPublished - 2014
MoE publication typeA4 Article in conference proceedings
EventBiennal Conference of the Australian Society of Animal Production - Canberra, Australia
Duration: 8 Sep 201412 Sep 2014
Conference number: 30

Publication series

NameAnimal production in Australia : proceedings of the biennial conference of the Australian Society of Animal Production
PublisherAustralian Society of Animal Production
Number30
ISSN (Electronic)0728-5965

Fields of Science

  • 1182 Biochemistry, cell and molecular biology
  • 412 Animal science, dairy science

Cite this

Kokkonen, T., Qin, N., Hilvo, M., Salin, S., Selim, S., Seppänen-Laakso, T., Taponen, J., Vanhatalo, A., & Elo, K. (2014). Effects of energy intake on lipidomic profile of liver and adipose tissue during the periparturient period in dairy cows. In S. Hatcher, G. L. Krebs, & B. V. B. Holman (Eds.), Harnessing the Ecology and Physiology of Herbivores: Proceedings of the 30th Biennial Conference of the Australian Society of Animal Production (pp. 271). (Animal production in Australia : proceedings of the biennial conference of the Australian Society of Animal Production; No. 30). Australian Society of Animal Production.