Effects of sugar beet chitinase IV on root-associated fungal community of transgenic silver birch in a field trial

Hanna-Leena Pasonen, Jinrong Lu, Anna-Maija Niskanen, Sanna-Kaisa Seppänen, Anna Rytkönen, Janne Raunio, Ari Pappinen, Risto Kasanen, Sari Timonen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Heterogenous chitinases have been introduced in many plant species with the aim to increase the resistance of plants to fungal diseases. We studied the effects of the heterologous expression of sugar beet chitinase IV on the intensity of ectomycorrhizal (ECM) colonization and the structure of fungal communities in the field trial of 15 transgenic and 8 wild-type silver birch (Betula pendula Roth) genotypes. Fungal sequences were separated in denaturing gradient gel electrophoresis and identified by sequencing the ITS1 region to reveal the operational taxonomic units. ECM colonization was less intense in 7 out of 15 transgenic lines than in the corresponding non-transgenic control plants, but the slight decrease in overall ECM colonization in transgenic lines could not be related to sugar beet chitinase IV expression or total endochitinase activity. One transgenic line showing fairly weak sugar beet chitinase IV expression without significantly increased total endochitinase activity differed significantly from the non-transgenic controls in the structure of fungal community. Five sequences belonging to three different fungal genera (Hebeloma, Inocybe, Laccaria) were indicative of wild-type genotypes, and one sequence (Lactarius) indicated one transgenic line. In cluster analysis, the non-transgenic control grouped together with the transgenic lines indicating that genotype was a more important factor determining the structure of fungal communities than the transgenic status of the plants. With the tested birch lines, no clear evidence for the effect of the heterologous expression of sugar beet chitinase IV on ECM colonization or the structure of fungal community was found.
Original languageEnglish
JournalPlanta
Volume230
Issue number5
Pages (from-to)973-983
Number of pages11
ISSN0032-0935
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 414 Agricultural biotechnology
  • 411 Agriculture and forestry

Cite this

Pasonen, Hanna-Leena ; Lu, Jinrong ; Niskanen, Anna-Maija ; Seppänen, Sanna-Kaisa ; Rytkönen, Anna ; Raunio, Janne ; Pappinen, Ari ; Kasanen, Risto ; Timonen, Sari. / Effects of sugar beet chitinase IV on root-associated fungal community of transgenic silver birch in a field trial. In: Planta. 2009 ; Vol. 230, No. 5. pp. 973-983.
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title = "Effects of sugar beet chitinase IV on root-associated fungal community of transgenic silver birch in a field trial",
abstract = "Heterogenous chitinases have been introduced in many plant species with the aim to increase the resistance of plants to fungal diseases. We studied the effects of the heterologous expression of sugar beet chitinase IV on the intensity of ectomycorrhizal (ECM) colonization and the structure of fungal communities in the field trial of 15 transgenic and 8 wild-type silver birch (Betula pendula Roth) genotypes. Fungal sequences were separated in denaturing gradient gel electrophoresis and identified by sequencing the ITS1 region to reveal the operational taxonomic units. ECM colonization was less intense in 7 out of 15 transgenic lines than in the corresponding non-transgenic control plants, but the slight decrease in overall ECM colonization in transgenic lines could not be related to sugar beet chitinase IV expression or total endochitinase activity. One transgenic line showing fairly weak sugar beet chitinase IV expression without significantly increased total endochitinase activity differed significantly from the non-transgenic controls in the structure of fungal community. Five sequences belonging to three different fungal genera (Hebeloma, Inocybe, Laccaria) were indicative of wild-type genotypes, and one sequence (Lactarius) indicated one transgenic line. In cluster analysis, the non-transgenic control grouped together with the transgenic lines indicating that genotype was a more important factor determining the structure of fungal communities than the transgenic status of the plants. With the tested birch lines, no clear evidence for the effect of the heterologous expression of sugar beet chitinase IV on ECM colonization or the structure of fungal community was found.",
keywords = "414 Agricultural biotechnology, 411 Agriculture and forestry",
author = "Hanna-Leena Pasonen and Jinrong Lu and Anna-Maija Niskanen and Sanna-Kaisa Sepp{\"a}nen and Anna Rytk{\"o}nen and Janne Raunio and Ari Pappinen and Risto Kasanen and Sari Timonen",
year = "2009",
doi = "10.1007/s00425-009-1005-4",
language = "English",
volume = "230",
pages = "973--983",
journal = "Planta",
issn = "0032-0935",
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Pasonen, H-L, Lu, J, Niskanen, A-M, Seppänen, S-K, Rytkönen, A, Raunio, J, Pappinen, A, Kasanen, R & Timonen, S 2009, 'Effects of sugar beet chitinase IV on root-associated fungal community of transgenic silver birch in a field trial' Planta, vol. 230, no. 5, pp. 973-983. https://doi.org/10.1007/s00425-009-1005-4

Effects of sugar beet chitinase IV on root-associated fungal community of transgenic silver birch in a field trial. / Pasonen, Hanna-Leena; Lu, Jinrong; Niskanen, Anna-Maija; Seppänen, Sanna-Kaisa; Rytkönen, Anna; Raunio, Janne; Pappinen, Ari; Kasanen, Risto; Timonen, Sari.

In: Planta, Vol. 230, No. 5, 2009, p. 973-983.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effects of sugar beet chitinase IV on root-associated fungal community of transgenic silver birch in a field trial

AU - Pasonen, Hanna-Leena

AU - Lu, Jinrong

AU - Niskanen, Anna-Maija

AU - Seppänen, Sanna-Kaisa

AU - Rytkönen, Anna

AU - Raunio, Janne

AU - Pappinen, Ari

AU - Kasanen, Risto

AU - Timonen, Sari

PY - 2009

Y1 - 2009

N2 - Heterogenous chitinases have been introduced in many plant species with the aim to increase the resistance of plants to fungal diseases. We studied the effects of the heterologous expression of sugar beet chitinase IV on the intensity of ectomycorrhizal (ECM) colonization and the structure of fungal communities in the field trial of 15 transgenic and 8 wild-type silver birch (Betula pendula Roth) genotypes. Fungal sequences were separated in denaturing gradient gel electrophoresis and identified by sequencing the ITS1 region to reveal the operational taxonomic units. ECM colonization was less intense in 7 out of 15 transgenic lines than in the corresponding non-transgenic control plants, but the slight decrease in overall ECM colonization in transgenic lines could not be related to sugar beet chitinase IV expression or total endochitinase activity. One transgenic line showing fairly weak sugar beet chitinase IV expression without significantly increased total endochitinase activity differed significantly from the non-transgenic controls in the structure of fungal community. Five sequences belonging to three different fungal genera (Hebeloma, Inocybe, Laccaria) were indicative of wild-type genotypes, and one sequence (Lactarius) indicated one transgenic line. In cluster analysis, the non-transgenic control grouped together with the transgenic lines indicating that genotype was a more important factor determining the structure of fungal communities than the transgenic status of the plants. With the tested birch lines, no clear evidence for the effect of the heterologous expression of sugar beet chitinase IV on ECM colonization or the structure of fungal community was found.

AB - Heterogenous chitinases have been introduced in many plant species with the aim to increase the resistance of plants to fungal diseases. We studied the effects of the heterologous expression of sugar beet chitinase IV on the intensity of ectomycorrhizal (ECM) colonization and the structure of fungal communities in the field trial of 15 transgenic and 8 wild-type silver birch (Betula pendula Roth) genotypes. Fungal sequences were separated in denaturing gradient gel electrophoresis and identified by sequencing the ITS1 region to reveal the operational taxonomic units. ECM colonization was less intense in 7 out of 15 transgenic lines than in the corresponding non-transgenic control plants, but the slight decrease in overall ECM colonization in transgenic lines could not be related to sugar beet chitinase IV expression or total endochitinase activity. One transgenic line showing fairly weak sugar beet chitinase IV expression without significantly increased total endochitinase activity differed significantly from the non-transgenic controls in the structure of fungal community. Five sequences belonging to three different fungal genera (Hebeloma, Inocybe, Laccaria) were indicative of wild-type genotypes, and one sequence (Lactarius) indicated one transgenic line. In cluster analysis, the non-transgenic control grouped together with the transgenic lines indicating that genotype was a more important factor determining the structure of fungal communities than the transgenic status of the plants. With the tested birch lines, no clear evidence for the effect of the heterologous expression of sugar beet chitinase IV on ECM colonization or the structure of fungal community was found.

KW - 414 Agricultural biotechnology

KW - 411 Agriculture and forestry

U2 - 10.1007/s00425-009-1005-4

DO - 10.1007/s00425-009-1005-4

M3 - Article

VL - 230

SP - 973

EP - 983

JO - Planta

JF - Planta

SN - 0032-0935

IS - 5

ER -