Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The endoplasmic reticulum ( ER) is a multifaceted cellular organelle both structurally and functionally, and its cell cycle - dependent morphological changes are poorly understood. Our quantitative confocal and EM analyses show that the ER undergoes dramatic reorganization during cell division in cultured mammalian cells as mitotic ER profiles become shorter and more branched. 3D modeling by electron tomography reveals that the abundant interphase structures, sheets, are lost and subsequently transform into a branched tubular network that remains continuous. This is confirmed by observing the most prominent ER subdomain, the nuclear envelope ( NE). A NE marker protein spreads to the mitotic ER tubules, although it does not show a homogenous distribution within the network. We mimicked the mitotic ER reorganization using puromycin to strip the membrane- bound ribosomes from the interphase ER corresponding to the observed loss of ribosomes normally occurring during mitosis. We propose that the structural changes in mitotic ER are linked to ribosomal action on the ER membranes.
Original languageEnglish
JournalJournal of Cell Biology
Volume179
Pages (from-to)895-909
Number of pages15
ISSN0021-9525
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Cite this

@article{818805d265884c1da7312e203debaef4,
title = "Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells",
abstract = "The endoplasmic reticulum ( ER) is a multifaceted cellular organelle both structurally and functionally, and its cell cycle - dependent morphological changes are poorly understood. Our quantitative confocal and EM analyses show that the ER undergoes dramatic reorganization during cell division in cultured mammalian cells as mitotic ER profiles become shorter and more branched. 3D modeling by electron tomography reveals that the abundant interphase structures, sheets, are lost and subsequently transform into a branched tubular network that remains continuous. This is confirmed by observing the most prominent ER subdomain, the nuclear envelope ( NE). A NE marker protein spreads to the mitotic ER tubules, although it does not show a homogenous distribution within the network. We mimicked the mitotic ER reorganization using puromycin to strip the membrane- bound ribosomes from the interphase ER corresponding to the observed loss of ribosomes normally occurring during mitosis. We propose that the structural changes in mitotic ER are linked to ribosomal action on the ER membranes.",
author = "Maija Puhka and Helena Vihinen and Merja Joensuu and Eija Jokitalo",
year = "2007",
doi = "10.1083/jcb.200705112",
language = "English",
volume = "179",
pages = "895--909",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "ROCKEFELLER UNIVERSITY PRESS",

}

Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells. / Puhka, Maija; Vihinen, Helena; Joensuu, Merja; Jokitalo, Eija.

In: Journal of Cell Biology, Vol. 179, 2007, p. 895-909.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells

AU - Puhka, Maija

AU - Vihinen, Helena

AU - Joensuu, Merja

AU - Jokitalo, Eija

PY - 2007

Y1 - 2007

N2 - The endoplasmic reticulum ( ER) is a multifaceted cellular organelle both structurally and functionally, and its cell cycle - dependent morphological changes are poorly understood. Our quantitative confocal and EM analyses show that the ER undergoes dramatic reorganization during cell division in cultured mammalian cells as mitotic ER profiles become shorter and more branched. 3D modeling by electron tomography reveals that the abundant interphase structures, sheets, are lost and subsequently transform into a branched tubular network that remains continuous. This is confirmed by observing the most prominent ER subdomain, the nuclear envelope ( NE). A NE marker protein spreads to the mitotic ER tubules, although it does not show a homogenous distribution within the network. We mimicked the mitotic ER reorganization using puromycin to strip the membrane- bound ribosomes from the interphase ER corresponding to the observed loss of ribosomes normally occurring during mitosis. We propose that the structural changes in mitotic ER are linked to ribosomal action on the ER membranes.

AB - The endoplasmic reticulum ( ER) is a multifaceted cellular organelle both structurally and functionally, and its cell cycle - dependent morphological changes are poorly understood. Our quantitative confocal and EM analyses show that the ER undergoes dramatic reorganization during cell division in cultured mammalian cells as mitotic ER profiles become shorter and more branched. 3D modeling by electron tomography reveals that the abundant interphase structures, sheets, are lost and subsequently transform into a branched tubular network that remains continuous. This is confirmed by observing the most prominent ER subdomain, the nuclear envelope ( NE). A NE marker protein spreads to the mitotic ER tubules, although it does not show a homogenous distribution within the network. We mimicked the mitotic ER reorganization using puromycin to strip the membrane- bound ribosomes from the interphase ER corresponding to the observed loss of ribosomes normally occurring during mitosis. We propose that the structural changes in mitotic ER are linked to ribosomal action on the ER membranes.

U2 - 10.1083/jcb.200705112

DO - 10.1083/jcb.200705112

M3 - Article

VL - 179

SP - 895

EP - 909

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

ER -