Projects per year
Abstract
Results. Successful production of active enzymes was attained, with spectral characteristics of high-spin class-II peroxidases. Recombinant Pr-MnP3 demonstrated high affinity to Mn2+, which was noticeably affected by single (D186H/N) and double (E40H+E44H) mutations. Without addition of Mn2+, Pr-MnP3 was able to oxidize ABTS and decolorize Reactive Blue 5. Pc-LiPH8, its Trp-radical site variants, and engineered CiP-LiP demonstrated conversion of veratryl alcohol and dimeric non-phenolic lignin-model compounds (arylglycerol-β-aryl ethers) with production of veratraldehyde, which is evidence for cation radical formation with subsequent Cα-Cβ cleavage. Pc-LiPH8 and CiP variants were able to effectively oxidize and convert the phenolic dimer (guaiacylglycerol-β–guaiacyl ether).
Conclusion. Our results demonstrate suitability of engineered MnP and LiP peroxidases for dye-decolorizing, and efficiency of LiP and its variants for activation and degradation of phenolic and non-phenolic lignin-like aryl ether-linked compounds.
Original language | English |
---|---|
Journal | Current Biotechnology |
Volume | 6 |
Issue number | 2 |
Pages (from-to) | 116-117 |
Number of pages | 12 |
ISSN | 2211-5501 |
DOIs | |
Publication status | Published - 2017 |
MoE publication type | A1 Journal article-refereed |
Fields of Science
- 414 Agricultural biotechnology
- Fungal biotechnology
- 219 Environmental biotechnology
- Lignocellulose biodegradation
- 1183 Plant biology, microbiology, virology
- Mycology
- Fungal genetics
- Heterologous expression
- 116 Chemical sciences
- 1182 Biochemistry, cell and molecular biology
Projects
- 4 Finished
-
Fungcolife: Fungal interactions and life-styles on wood
Lundell, T. (Project manager), Mali, T. (Participant), Mattila, H. (Participant), Shah, F. (Participant) & Mäkinen, M. (Participant)
01/09/2015 → 31/12/2019
Project: Research project
-
Ox-Red: Functions and quenching of fungal oxidoreductases in a wood white-rot decay fungus
Lundell, T. (Project manager), Oksanen, I. (Participant), Kuuskeri, J. (Participant), Mäkelä, M. (Participant), Hilden, K. (Participant), Mäkinen, M. (Participant) & Paulin, L. (Participant)
01/01/2011 → 30/06/2015
Project: Research project
-
Oxidoreductases and natural type mediators in fungal conversion of lignocellulose-Academy of Finland
Hatakka, A. (Participant), Sipilä, J. (Project manager), Lundell, T. (Participant) & Nousiainen, P. A. (Participant)
01/01/2010 → 31/12/2012
Project: Research project
Equipment
-
Microbial Domain Biological Resource Centre HAMBI (LS-RIA/ HUBCRI)
Friman, V.-P. (Manager) & Oivanen, P. (Operator)
Department of MicrobiologyFacility/equipment: Core Facility