Enzyme activity profiles produced on wood and straw by four fungi of different decay strategies

Eliana Estefanía Veloz Villavicencio, Tuulia Mali, Hans Mattila, Taina Lundell

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Four well-studied saprotrophic Basidiomycota Agaricomycetes species with different decay strategies were cultivated on solid lignocellulose substrates to compare their extracellular decomposing carbohydrate-active and lignin-attacking enzyme production profiles. Two Polyporales species, the white rot fungus Phlebia radiata and brown rot fungus Fomitopsis pinicola, as well as one Agaricales species, the intermediate “grey” rot fungus Schizophyllum commune, were cultivated on birch wood pieces for 12 weeks, whereas the second Agaricales species, the litter-decomposing fungus Coprinopsis cinerea was cultivated on barley straw for 6 weeks under laboratory conditions. During 3 months of growth on birch wood, only the white rot fungus P. radiata produced high laccase and MnP activities. The brown rot fungus F. pinicola demonstrated notable production of xylanase activity up to 43 nkat/mL on birch wood, together with moderate β-glucosidase and endoglucanase cellulolytic activities. The intermediate rot fungus S. commune was the strongest producer of β-glucosidase with activities up to 54 nkat/mL, and a notable producer of xylanase activity, even up to 620 nkat/mL, on birch wood. Low lignin-attacking but moderate activities against cellulose and hemicellulose were observed with the litter-decomposer C. cinerea on barley straw. Overall, our results imply that plant cell wall decomposition ability of taxonomically and ecologically divergent fungi is in line with their enzymatic decay strategy, which is fundamental in understanding their physiology and potential for biotechnological applications.
Original languageEnglish
Article number73
JournalMicroorganisms
Volume8
Issue number1
ISSN2076-2607
DOIs
Publication statusPublished - 2 Jan 2020
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • Basidiomycota
  • Wood decay fungi
  • Lignocellulose
  • Biodegradation
  • fungal enzymes
  • Carbohydrate active enzymes
  • Laccase
  • Manganese peroxidase
  • enzyme activity profile
  • 414 Agricultural biotechnology
  • Wood biodegradation
  • Waste biomass

Cite this

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title = "Enzyme activity profiles produced on wood and straw by four fungi of different decay strategies",
abstract = "Four well-studied saprotrophic Basidiomycota Agaricomycetes species with different decay strategies were cultivated on solid lignocellulose substrates to compare their extracellular decomposing carbohydrate-active and lignin-attacking enzyme production profiles. Two Polyporales species, the white rot fungus Phlebia radiata and brown rot fungus Fomitopsis pinicola, as well as one Agaricales species, the intermediate “grey” rot fungus Schizophyllum commune, were cultivated on birch wood pieces for 12 weeks, whereas the second Agaricales species, the litter-decomposing fungus Coprinopsis cinerea was cultivated on barley straw for 6 weeks under laboratory conditions. During 3 months of growth on birch wood, only the white rot fungus P. radiata produced high laccase and MnP activities. The brown rot fungus F. pinicola demonstrated notable production of xylanase activity up to 43 nkat/mL on birch wood, together with moderate β-glucosidase and endoglucanase cellulolytic activities. The intermediate rot fungus S. commune was the strongest producer of β-glucosidase with activities up to 54 nkat/mL, and a notable producer of xylanase activity, even up to 620 nkat/mL, on birch wood. Low lignin-attacking but moderate activities against cellulose and hemicellulose were observed with the litter-decomposer C. cinerea on barley straw. Overall, our results imply that plant cell wall decomposition ability of taxonomically and ecologically divergent fungi is in line with their enzymatic decay strategy, which is fundamental in understanding their physiology and potential for biotechnological applications.",
keywords = "1183 Plant biology, microbiology, virology, Basidiomycota, Wood decay fungi, Lignocellulose, Biodegradation, fungal enzymes, Carbohydrate active enzymes, Laccase, Manganese peroxidase, enzyme activity profile, 414 Agricultural biotechnology, Wood biodegradation, Waste biomass",
author = "{Veloz Villavicencio}, {Eliana Estefan{\'i}a} and Tuulia Mali and Hans Mattila and Taina Lundell",
year = "2020",
month = "1",
day = "2",
doi = "10.3390/microorganisms8010073",
language = "English",
volume = "8",
journal = "Microorganisms",
issn = "2076-2607",
publisher = "MDPI",
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}

Enzyme activity profiles produced on wood and straw by four fungi of different decay strategies. / Veloz Villavicencio, Eliana Estefanía; Mali, Tuulia; Mattila, Hans; Lundell, Taina.

In: Microorganisms, Vol. 8, No. 1, 73, 02.01.2020.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enzyme activity profiles produced on wood and straw by four fungi of different decay strategies

AU - Veloz Villavicencio, Eliana Estefanía

AU - Mali, Tuulia

AU - Mattila, Hans

AU - Lundell, Taina

PY - 2020/1/2

Y1 - 2020/1/2

N2 - Four well-studied saprotrophic Basidiomycota Agaricomycetes species with different decay strategies were cultivated on solid lignocellulose substrates to compare their extracellular decomposing carbohydrate-active and lignin-attacking enzyme production profiles. Two Polyporales species, the white rot fungus Phlebia radiata and brown rot fungus Fomitopsis pinicola, as well as one Agaricales species, the intermediate “grey” rot fungus Schizophyllum commune, were cultivated on birch wood pieces for 12 weeks, whereas the second Agaricales species, the litter-decomposing fungus Coprinopsis cinerea was cultivated on barley straw for 6 weeks under laboratory conditions. During 3 months of growth on birch wood, only the white rot fungus P. radiata produced high laccase and MnP activities. The brown rot fungus F. pinicola demonstrated notable production of xylanase activity up to 43 nkat/mL on birch wood, together with moderate β-glucosidase and endoglucanase cellulolytic activities. The intermediate rot fungus S. commune was the strongest producer of β-glucosidase with activities up to 54 nkat/mL, and a notable producer of xylanase activity, even up to 620 nkat/mL, on birch wood. Low lignin-attacking but moderate activities against cellulose and hemicellulose were observed with the litter-decomposer C. cinerea on barley straw. Overall, our results imply that plant cell wall decomposition ability of taxonomically and ecologically divergent fungi is in line with their enzymatic decay strategy, which is fundamental in understanding their physiology and potential for biotechnological applications.

AB - Four well-studied saprotrophic Basidiomycota Agaricomycetes species with different decay strategies were cultivated on solid lignocellulose substrates to compare their extracellular decomposing carbohydrate-active and lignin-attacking enzyme production profiles. Two Polyporales species, the white rot fungus Phlebia radiata and brown rot fungus Fomitopsis pinicola, as well as one Agaricales species, the intermediate “grey” rot fungus Schizophyllum commune, were cultivated on birch wood pieces for 12 weeks, whereas the second Agaricales species, the litter-decomposing fungus Coprinopsis cinerea was cultivated on barley straw for 6 weeks under laboratory conditions. During 3 months of growth on birch wood, only the white rot fungus P. radiata produced high laccase and MnP activities. The brown rot fungus F. pinicola demonstrated notable production of xylanase activity up to 43 nkat/mL on birch wood, together with moderate β-glucosidase and endoglucanase cellulolytic activities. The intermediate rot fungus S. commune was the strongest producer of β-glucosidase with activities up to 54 nkat/mL, and a notable producer of xylanase activity, even up to 620 nkat/mL, on birch wood. Low lignin-attacking but moderate activities against cellulose and hemicellulose were observed with the litter-decomposer C. cinerea on barley straw. Overall, our results imply that plant cell wall decomposition ability of taxonomically and ecologically divergent fungi is in line with their enzymatic decay strategy, which is fundamental in understanding their physiology and potential for biotechnological applications.

KW - 1183 Plant biology, microbiology, virology

KW - Basidiomycota

KW - Wood decay fungi

KW - Lignocellulose

KW - Biodegradation

KW - fungal enzymes

KW - Carbohydrate active enzymes

KW - Laccase

KW - Manganese peroxidase

KW - enzyme activity profile

KW - 414 Agricultural biotechnology

KW - Wood biodegradation

KW - Waste biomass

U2 - 10.3390/microorganisms8010073

DO - 10.3390/microorganisms8010073

M3 - Article

VL - 8

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 1

M1 - 73

ER -