Projects per year
Abstract
Four well-studied saprotrophic Basidiomycota Agaricomycetes species with different decay strategies were cultivated on solid lignocellulose substrates to compare their extracellular decomposing carbohydrate-active and lignin-attacking enzyme production profiles. Two Polyporales species, the white rot fungus Phlebia radiata and brown rot fungus Fomitopsis pinicola, as well as one Agaricales species, the intermediate “grey” rot fungus Schizophyllum commune, were cultivated on birch wood pieces for 12 weeks, whereas the second Agaricales species, the litter-decomposing fungus Coprinopsis cinerea was cultivated on barley straw for 6 weeks under laboratory conditions. During 3 months of growth on birch wood, only the white rot fungus P. radiata produced high laccase and MnP activities. The brown rot fungus F. pinicola demonstrated notable production of xylanase activity up to 43 nkat/mL on birch wood, together with moderate β-glucosidase and endoglucanase cellulolytic activities. The intermediate rot fungus S. commune was the strongest producer of β-glucosidase with activities up to 54 nkat/mL, and a notable producer of xylanase activity, even up to 620 nkat/mL, on birch wood. Low lignin-attacking but moderate activities against cellulose and hemicellulose were observed with the litter-decomposer C. cinerea on barley straw. Overall, our results imply that plant cell wall decomposition ability of taxonomically and ecologically divergent fungi is in line with their enzymatic decay strategy, which is fundamental in understanding their physiology and potential for biotechnological applications.
Original language | English |
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Article number | 73 |
Journal | Microorganisms |
Volume | 8 |
Issue number | 1 |
Number of pages | 18 |
ISSN | 2076-2607 |
DOIs | |
Publication status | Published - 2 Jan 2020 |
MoE publication type | A1 Journal article-refereed |
Fields of Science
- 414 Agricultural biotechnology
- Wood biodegradation
- Waste biomass
- 11831 Plant biology
- Basidiomycota
- wood decay fungi
- lignocellulose
- biodegradation
- carbohydrate active enzymes
- laccase
- manganese peroxidase
- enzyme activity assays
- organic acids
- oxalic acid
- COPRINOPSIS-CINEREA
- GENOME SEQUENCE
- LIGNIN
- DEGRADATION
- MECHANISMS
- EVOLUTION
- fungal enzymes
- enzyme activeity profile
Projects
- 3 Finished
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Biofungi - kääpäsienten fermentaatiokyvyn ja aineenvaihdunnan reittien avaus jätteiden muuntamisessa
Lundell, T., Kiviniemi, E. A., Mattila, H., Christodoulou, M., Hurtado López, N., Kiviniemi, E. A., Laine, T. A. K., Mali, T., Mattila, H., Mikkola, A. H., Pulkkanen, R. E. & Österman-Udd, J.
01/01/2019 → 31/08/2022
Project: Foundations (Private Foundations, Non-Profit Foundations, Charitable Trusts)
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Fungcolife: Fungal interactions and life-styles on wood
Lundell, T., Mali, T., Mattila, H., Shah, F. & Mäkinen, M.
01/09/2015 → 31/12/2019
Project: Research project
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Fungal degradation and conversion of recyclable lignocelluloses
01/06/2015 → 31/12/2018
Project: Research project
Equipment
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HAMBI Microbial Culture Collection, University of Helsinki
Pekka Oivanen (Operator) & Kaarina Sivonen (Manager)
Department of MicrobiologyFacility/equipment: Equipment