Abstract
Gerbera jamesonii is one of the most popular ornamental plants in the world. This study
comprises of three different phases for shoot regeneration. Two different color varieties of
G.Jamesonii, one had red, and the other yellow petals plants were used in this study. The plant's
leaf with midrib, flower bud, and flower stalks was used as explants in this experiment. 3rd
position leaf from the top of the both variety of plant yielded best results for in vitro
regeneration, with 70% for red variety and 60% for yellow respectively. In case of flower buds
and flower stalks, 6-7 days old flower buds and flower stalks gave best results in the in vitro
regeneration studies with 80% response rate for red variety and 60% for the yellow variety. The
given response rate for flower stalks were 38% for the red variety and 50% for yellow variety.
The above three different types of explants were cultured in the MS medium containing different
hormonal combination and concentrations in vitro regeneration. The best results were obtained
with the combination of 5 mg/l BAP with 1mg/l NAA which formed multiple shoots from all the
explants. The shoot proliferation was started within 6-7 weeks after inoculation of explants in the
suitable medium. Our study showed the maximum mean no. of shoot from the leaf with mid rib
explant and the no. was found to be 7.75 per explant. Prospects may include newer methods for
root regeneration for both of these varieties of Gerbera to complete the regeneration protocol for
this plant species.
comprises of three different phases for shoot regeneration. Two different color varieties of
G.Jamesonii, one had red, and the other yellow petals plants were used in this study. The plant's
leaf with midrib, flower bud, and flower stalks was used as explants in this experiment. 3rd
position leaf from the top of the both variety of plant yielded best results for in vitro
regeneration, with 70% for red variety and 60% for yellow respectively. In case of flower buds
and flower stalks, 6-7 days old flower buds and flower stalks gave best results in the in vitro
regeneration studies with 80% response rate for red variety and 60% for the yellow variety. The
given response rate for flower stalks were 38% for the red variety and 50% for yellow variety.
The above three different types of explants were cultured in the MS medium containing different
hormonal combination and concentrations in vitro regeneration. The best results were obtained
with the combination of 5 mg/l BAP with 1mg/l NAA which formed multiple shoots from all the
explants. The shoot proliferation was started within 6-7 weeks after inoculation of explants in the
suitable medium. Our study showed the maximum mean no. of shoot from the leaf with mid rib
explant and the no. was found to be 7.75 per explant. Prospects may include newer methods for
root regeneration for both of these varieties of Gerbera to complete the regeneration protocol for
this plant species.
| Original language | English |
|---|---|
| Journal | International Journal of Business, Social and Scientific Research |
| Volume | 7 |
| Issue number | 1 |
| Pages (from-to) | 62-67 |
| Number of pages | 5 |
| ISSN | 2519-5530 |
| Publication status | Published - 31 Aug 2018 |
| Externally published | Yes |
| MoE publication type | A1 Journal article-refereed |
Fields of Science
- 1183 Plant biology, microbiology, virology