Evaluation of normalization reference genes for RT-qPCR analysis of spo0A and four sporulation sigma factor genes in Clostridium botulinum Group I strain ATCC 3502

David G Kirk; Eveliina Palonen; Hannu Korkeala; Miia Lindström

doi:10.1016/j.anaerobe.2013.12.003

Evaluation of normalization reference genes for RT-qPCR analysis of spo0A and four sporulation sigma factor genes in Clostridium botulinum Group I strain ATCC 3502

David G Kirk, Eveliina Palonen, Hannu Korkeala, Miia Lindström

Research output: Contribution to journal › Article › Scientific › peer-review

Original language	English
Journal	Anaerobe
Volume	26
Pages (from-to)	14-19
Number of pages	6
ISSN	1075-9964
DOIs	https://doi.org/10.1016/j.anaerobe.2013.12.003
Publication status	Published - 6 Jan 2014
MoE publication type	A1 Journal article-refereed

Fields of Science

413 Veterinary science
Clostridium botulinum
Sporulation
Reference gene
RT-qPCR
BACILLUS-SUBTILIS SPORULATION
REAL-TIME PCR
HOUSEKEEPING GENES
CARBON-DIOXIDE
EXPRESSION
TRANSCRIPTION
NEUROTOXIN
INVOLVEMENT
GROWTH
PERFRINGENS

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10.1016/j.anaerobe.2013.12.003

A Clostridal Biology Network to Facilitate European-wide Medical Countermeasures and Commercial Exploitation (CLOSTNET)
Lindström, M., Kirk, D., Zhang, Z., Minton, N., Bahl, H., Titball, R., Liebl, W., Rupnik, M., Mastrantonio, P., Tardiff, C., Soucaille, P., McClure, P. & Telford, J.
Unknown funder
01/01/2010 → 31/12/2013
Project: Research project

Cite this

@article{479d89983d2f420194e75d6e8604fc53,

title = "Evaluation of normalization reference genes for RT-qPCR analysis of spo0A and four sporulation sigma factor genes in Clostridium botulinum Group I strain ATCC 3502",

keywords = "413 Veterinary science, Clostridium botulinum, Sporulation, Reference gene, RT-qPCR, BACILLUS-SUBTILIS SPORULATION, REAL-TIME PCR, HOUSEKEEPING GENES, CARBON-DIOXIDE, EXPRESSION, TRANSCRIPTION, NEUROTOXIN, INVOLVEMENT, GROWTH, PERFRINGENS",

author = "Kirk, {David G} and Eveliina Palonen and Hannu Korkeala and Miia Lindstr{\"o}m",

year = "2014",

month = jan,

day = "6",

doi = "10.1016/j.anaerobe.2013.12.003",

language = "English",

volume = "26",

pages = "14--19",

journal = "Anaerobe",

issn = "1075-9964",

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}

TY - JOUR

T1 - Evaluation of normalization reference genes for RT-qPCR analysis of spo0A and four sporulation sigma factor genes in Clostridium botulinum Group I strain ATCC 3502

AU - Kirk, David G

AU - Palonen, Eveliina

AU - Korkeala, Hannu

AU - Lindström, Miia

PY - 2014/1/6

Y1 - 2014/1/6

KW - 413 Veterinary science

KW - Clostridium botulinum

KW - Sporulation

KW - Reference gene

KW - RT-qPCR

KW - BACILLUS-SUBTILIS SPORULATION

KW - REAL-TIME PCR

KW - HOUSEKEEPING GENES

KW - CARBON-DIOXIDE

KW - EXPRESSION

KW - TRANSCRIPTION

KW - NEUROTOXIN

KW - INVOLVEMENT

KW - GROWTH

KW - PERFRINGENS

U2 - 10.1016/j.anaerobe.2013.12.003

DO - 10.1016/j.anaerobe.2013.12.003

M3 - Article

VL - 26

SP - 14

EP - 19

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

ER -

Evaluation of normalization reference genes for RT-qPCR analysis of spo0A and four sporulation sigma factor genes in Clostridium botulinum Group I strain ATCC 3502

Fields of Science

Access to Document

Projects

A Clostridal Biology Network to Facilitate European-wide Medical Countermeasures and Commercial Exploitation (CLOSTNET)

Cite this