Expression of the nodularin synthetase genes in the Baltic Sea bloom-former cyanobacterium Nodularia spumigena strain AV1

Sara Jonasson, Simina Vintila, Kaarina Sivonen, Rehab El-Shehawy

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Cyanobacterial blooms in the Baltic Sea are a common phenomenon and are formed by the heterocystous, filamentous species Nodularia spumigena. The toxicity of these blooms is attributed to the hepatotoxin nodularin, produced by N. spumigena. Little is known regarding the regulatory mechanisms or environmental signaling that control nodularin production. Here we report the characterization of the transcriptional expression pattern of the nodularin synthetase gene cluster (nda) during phosphate depletion, and nitrogen supplementation. Real-time PCR analysis of these genes revealed that while cells continuously expressed the nda cluster, the expression of all nda genes increased when cells were subjected to phosphate depletion, and decreased in the presence of ammonium. In contrast to the shifts in expression, the intracellular and extracellular nodularin concentrations did not vary significantly during the treatments.
Original languageEnglish
JournalFEMS Microbiology Ecology
Volume65
Issue number1
Pages (from-to)31-39
Number of pages9
ISSN0168-6496
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fields of Science

  • Nodularia spumigena

Cite this

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title = "Expression of the nodularin synthetase genes in the Baltic Sea bloom-former cyanobacterium Nodularia spumigena strain AV1",
abstract = "Cyanobacterial blooms in the Baltic Sea are a common phenomenon and are formed by the heterocystous, filamentous species Nodularia spumigena. The toxicity of these blooms is attributed to the hepatotoxin nodularin, produced by N. spumigena. Little is known regarding the regulatory mechanisms or environmental signaling that control nodularin production. Here we report the characterization of the transcriptional expression pattern of the nodularin synthetase gene cluster (nda) during phosphate depletion, and nitrogen supplementation. Real-time PCR analysis of these genes revealed that while cells continuously expressed the nda cluster, the expression of all nda genes increased when cells were subjected to phosphate depletion, and decreased in the presence of ammonium. In contrast to the shifts in expression, the intracellular and extracellular nodularin concentrations did not vary significantly during the treatments.",
keywords = "Nodularia spumigena, Nodularia spumigena, Nodularia spumigena",
author = "Sara Jonasson and Simina Vintila and Kaarina Sivonen and Rehab El-Shehawy",
year = "2008",
doi = "10.1111/j.1574-6941.2008.00499.x",
language = "English",
volume = "65",
pages = "31--39",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
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Expression of the nodularin synthetase genes in the Baltic Sea bloom-former cyanobacterium Nodularia spumigena strain AV1. / Jonasson, Sara; Vintila, Simina; Sivonen, Kaarina; El-Shehawy, Rehab.

In: FEMS Microbiology Ecology, Vol. 65, No. 1, 2008, p. 31-39.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Expression of the nodularin synthetase genes in the Baltic Sea bloom-former cyanobacterium Nodularia spumigena strain AV1

AU - Jonasson, Sara

AU - Vintila, Simina

AU - Sivonen, Kaarina

AU - El-Shehawy, Rehab

PY - 2008

Y1 - 2008

N2 - Cyanobacterial blooms in the Baltic Sea are a common phenomenon and are formed by the heterocystous, filamentous species Nodularia spumigena. The toxicity of these blooms is attributed to the hepatotoxin nodularin, produced by N. spumigena. Little is known regarding the regulatory mechanisms or environmental signaling that control nodularin production. Here we report the characterization of the transcriptional expression pattern of the nodularin synthetase gene cluster (nda) during phosphate depletion, and nitrogen supplementation. Real-time PCR analysis of these genes revealed that while cells continuously expressed the nda cluster, the expression of all nda genes increased when cells were subjected to phosphate depletion, and decreased in the presence of ammonium. In contrast to the shifts in expression, the intracellular and extracellular nodularin concentrations did not vary significantly during the treatments.

AB - Cyanobacterial blooms in the Baltic Sea are a common phenomenon and are formed by the heterocystous, filamentous species Nodularia spumigena. The toxicity of these blooms is attributed to the hepatotoxin nodularin, produced by N. spumigena. Little is known regarding the regulatory mechanisms or environmental signaling that control nodularin production. Here we report the characterization of the transcriptional expression pattern of the nodularin synthetase gene cluster (nda) during phosphate depletion, and nitrogen supplementation. Real-time PCR analysis of these genes revealed that while cells continuously expressed the nda cluster, the expression of all nda genes increased when cells were subjected to phosphate depletion, and decreased in the presence of ammonium. In contrast to the shifts in expression, the intracellular and extracellular nodularin concentrations did not vary significantly during the treatments.

KW - Nodularia spumigena

KW - Nodularia spumigena

KW - Nodularia spumigena

U2 - 10.1111/j.1574-6941.2008.00499.x

DO - 10.1111/j.1574-6941.2008.00499.x

M3 - Article

VL - 65

SP - 31

EP - 39

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 1

ER -