Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis

Sacha Escamez, Simon Stael, Julia Vainonen, Patrick Willems, Huiting Jin, Sachie Kimura, Frank Van Breusegem, Kris Gevaert, Michael Alois Wrzaczek, Hannele Tuominen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

During plant vascular development, xylem tracheary elements (TEs) form water-conducting, empty pipes by genetically regulated cell death. Cell death is prevented from spreading to non-TEs by unidentified intercellular mechanisms, downstream of METACASPASE9 (MC9)-mediated regulation of autophagy in TEs. Here, we identified differentially abundant extracellular peptides in vascular-differentiating wild-type and MC9-down-regulated Arabidopsis cell suspensions. A peptide named Kratos rescued the abnormally high ectopic non-TE death resulting from either MC9 knockout or TE-specific overexpression of the ATG5 autophagy protein during experimentally induced vascular differentiation in Arabidopsis cotyledons. Kratos also reduced cell death following mechanical damage and extracellular ROS production in Arabidopsis leaves. Stress-induced but not vascular non-TE cell death was enhanced by another identified peptide, named Bia. Bia is therefore reminiscent of several known plant cell death-inducing peptides acting as damage-associated molecular patterns. In contrast, Kratos plays a novel extracellular cell survival role in the context of development and during stress response.
Original languageEnglish
JournalJournal of Experimental Botany
Volume70
Issue number7
Pages (from-to)2199-2210
Number of pages12
ISSN0022-0957
DOIs
Publication statusPublished - 15 Mar 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • APOPTOSIS
  • AUTOLYSIS
  • AUTOPHAGY
  • Arabidopsis
  • IDENTIFICATION
  • INFLAMMATION
  • MECHANISM
  • PATHWAY
  • PROTEIN
  • REVEALS
  • SPREAD
  • autophagy
  • cell death
  • peptide
  • peptidomics
  • programmed cell death
  • stress response
  • vascular development
  • xylem
  • 1183 Plant biology, microbiology, virology

Cite this

Escamez, Sacha ; Stael, Simon ; Vainonen, Julia ; Willems, Patrick ; Jin, Huiting ; Kimura, Sachie ; Van Breusegem, Frank ; Gevaert, Kris ; Wrzaczek, Michael Alois ; Tuominen, Hannele. / Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis. In: Journal of Experimental Botany. 2019 ; Vol. 70, No. 7. pp. 2199-2210.
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title = "Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis",
abstract = "During plant vascular development, xylem tracheary elements (TEs) form water-conducting, empty pipes by genetically regulated cell death. Cell death is prevented from spreading to non-TEs by unidentified intercellular mechanisms, downstream of METACASPASE9 (MC9)-mediated regulation of autophagy in TEs. Here, we identified differentially abundant extracellular peptides in vascular-differentiating wild-type and MC9-down-regulated Arabidopsis cell suspensions. A peptide named Kratos rescued the abnormally high ectopic non-TE death resulting from either MC9 knockout or TE-specific overexpression of the ATG5 autophagy protein during experimentally induced vascular differentiation in Arabidopsis cotyledons. Kratos also reduced cell death following mechanical damage and extracellular ROS production in Arabidopsis leaves. Stress-induced but not vascular non-TE cell death was enhanced by another identified peptide, named Bia. Bia is therefore reminiscent of several known plant cell death-inducing peptides acting as damage-associated molecular patterns. In contrast, Kratos plays a novel extracellular cell survival role in the context of development and during stress response.",
keywords = "APOPTOSIS, AUTOLYSIS, AUTOPHAGY, Arabidopsis, IDENTIFICATION, INFLAMMATION, MECHANISM, PATHWAY, PROTEIN, REVEALS, SPREAD, autophagy, cell death, peptide, peptidomics, programmed cell death, stress response, vascular development, xylem, 1183 Plant biology, microbiology, virology",
author = "Sacha Escamez and Simon Stael and Julia Vainonen and Patrick Willems and Huiting Jin and Sachie Kimura and {Van Breusegem}, Frank and Kris Gevaert and Wrzaczek, {Michael Alois} and Hannele Tuominen",
year = "2019",
month = "3",
day = "15",
doi = "10.1093/jxb/erz021",
language = "English",
volume = "70",
pages = "2199--2210",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
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Escamez, S, Stael, S, Vainonen, J, Willems, P, Jin, H, Kimura, S, Van Breusegem, F, Gevaert, K, Wrzaczek, MA & Tuominen, H 2019, 'Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis' Journal of Experimental Botany, vol. 70, no. 7, pp. 2199-2210. https://doi.org/10.1093/jxb/erz021

Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis. / Escamez, Sacha; Stael, Simon; Vainonen, Julia; Willems, Patrick; Jin, Huiting; Kimura, Sachie; Van Breusegem, Frank; Gevaert, Kris; Wrzaczek, Michael Alois; Tuominen, Hannele.

In: Journal of Experimental Botany, Vol. 70, No. 7, 15.03.2019, p. 2199-2210.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis

AU - Escamez, Sacha

AU - Stael, Simon

AU - Vainonen, Julia

AU - Willems, Patrick

AU - Jin, Huiting

AU - Kimura, Sachie

AU - Van Breusegem, Frank

AU - Gevaert, Kris

AU - Wrzaczek, Michael Alois

AU - Tuominen, Hannele

PY - 2019/3/15

Y1 - 2019/3/15

N2 - During plant vascular development, xylem tracheary elements (TEs) form water-conducting, empty pipes by genetically regulated cell death. Cell death is prevented from spreading to non-TEs by unidentified intercellular mechanisms, downstream of METACASPASE9 (MC9)-mediated regulation of autophagy in TEs. Here, we identified differentially abundant extracellular peptides in vascular-differentiating wild-type and MC9-down-regulated Arabidopsis cell suspensions. A peptide named Kratos rescued the abnormally high ectopic non-TE death resulting from either MC9 knockout or TE-specific overexpression of the ATG5 autophagy protein during experimentally induced vascular differentiation in Arabidopsis cotyledons. Kratos also reduced cell death following mechanical damage and extracellular ROS production in Arabidopsis leaves. Stress-induced but not vascular non-TE cell death was enhanced by another identified peptide, named Bia. Bia is therefore reminiscent of several known plant cell death-inducing peptides acting as damage-associated molecular patterns. In contrast, Kratos plays a novel extracellular cell survival role in the context of development and during stress response.

AB - During plant vascular development, xylem tracheary elements (TEs) form water-conducting, empty pipes by genetically regulated cell death. Cell death is prevented from spreading to non-TEs by unidentified intercellular mechanisms, downstream of METACASPASE9 (MC9)-mediated regulation of autophagy in TEs. Here, we identified differentially abundant extracellular peptides in vascular-differentiating wild-type and MC9-down-regulated Arabidopsis cell suspensions. A peptide named Kratos rescued the abnormally high ectopic non-TE death resulting from either MC9 knockout or TE-specific overexpression of the ATG5 autophagy protein during experimentally induced vascular differentiation in Arabidopsis cotyledons. Kratos also reduced cell death following mechanical damage and extracellular ROS production in Arabidopsis leaves. Stress-induced but not vascular non-TE cell death was enhanced by another identified peptide, named Bia. Bia is therefore reminiscent of several known plant cell death-inducing peptides acting as damage-associated molecular patterns. In contrast, Kratos plays a novel extracellular cell survival role in the context of development and during stress response.

KW - APOPTOSIS

KW - AUTOLYSIS

KW - AUTOPHAGY

KW - Arabidopsis

KW - IDENTIFICATION

KW - INFLAMMATION

KW - MECHANISM

KW - PATHWAY

KW - PROTEIN

KW - REVEALS

KW - SPREAD

KW - autophagy

KW - cell death

KW - peptide

KW - peptidomics

KW - programmed cell death

KW - stress response

KW - vascular development

KW - xylem

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1093/jxb/erz021

DO - 10.1093/jxb/erz021

M3 - Article

VL - 70

SP - 2199

EP - 2210

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 7

ER -