Fertilizing with Animal Manure Disseminates Antibiotic Resistance Genes to the Farm Environment

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The dissemination of antibiotic resistance genes to the environment is an important factor causing increased prevalence of resistant pathogens. Manure is an important fertilizer, but it contains diverse resistance genes. Therefore, its application to fields may lead to increased abundance of resistance genes in the environment. Farming environments exposed to animal manure have not been studied extensively in countries with comparably low antibiotic use, such as Finland. The effects of manure storage and application to fields on the abundance of resistance genes were studied on two dairy cattle farms and two swine farms in southern Finland. Samples were taken from farms during the 2013 cropping season. Copy numbers of carbapenem (blaOXA-58), sulfonamide (sul1), and tetracycline (tetM) resistance genes were measured with quantitative polymerase chain reaction, and the data were analyzed using linear mixed models. The relative abundance of antibiotic resistance genes increased about fourfold in soil after manure application. Carbapenemase encoding blaOXA-58 was detected on all of the studied farms, which indicated that the gene is dispersed in the farm environment. The relative abundance of antibiotic resistance genes increased in stored manure compared with fresh manure roughly fivefold. This study shows that antibiotic resistance genes are disseminated on Finnish production animal farms. The spreading of resistance genes in farm-associated environments could possibly be limited by experimenting with new manure handling methods that could reduce the abundance of the genes in manure used for land application
Original languageEnglish
JournalJournal of Environmental Quality
Volume45
Issue number2
Pages (from-to)488-493
Number of pages6
ISSN0047-2425
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1172 Environmental sciences

Cite this

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title = "Fertilizing with Animal Manure Disseminates Antibiotic Resistance Genes to the Farm Environment",
abstract = "The dissemination of antibiotic resistance genes to the environment is an important factor causing increased prevalence of resistant pathogens. Manure is an important fertilizer, but it contains diverse resistance genes. Therefore, its application to fields may lead to increased abundance of resistance genes in the environment. Farming environments exposed to animal manure have not been studied extensively in countries with comparably low antibiotic use, such as Finland. The effects of manure storage and application to fields on the abundance of resistance genes were studied on two dairy cattle farms and two swine farms in southern Finland. Samples were taken from farms during the 2013 cropping season. Copy numbers of carbapenem (blaOXA-58), sulfonamide (sul1), and tetracycline (tetM) resistance genes were measured with quantitative polymerase chain reaction, and the data were analyzed using linear mixed models. The relative abundance of antibiotic resistance genes increased about fourfold in soil after manure application. Carbapenemase encoding blaOXA-58 was detected on all of the studied farms, which indicated that the gene is dispersed in the farm environment. The relative abundance of antibiotic resistance genes increased in stored manure compared with fresh manure roughly fivefold. This study shows that antibiotic resistance genes are disseminated on Finnish production animal farms. The spreading of resistance genes in farm-associated environments could possibly be limited by experimenting with new manure handling methods that could reduce the abundance of the genes in manure used for land application",
keywords = "1172 Environmental sciences",
author = "Matti Ruuskanen and Muurinen, {Sonja Johanna} and Axel Meierjohan and P{\"a}rn{\"a}nen, {Katariina Mariia Margare} and Tamminen, {Manu Valtteri} and Lyra, {Christina Darling} and Leif Kronberg and Virta, {Marko Petri Juhani}",
year = "2016",
doi = "10.2134/jeq2015.05.0250",
language = "English",
volume = "45",
pages = "488--493",
journal = "Journal of Environmental Quality",
issn = "0047-2425",
publisher = "American Society of Agronomy",
number = "2",

}

Fertilizing with Animal Manure Disseminates Antibiotic Resistance Genes to the Farm Environment. / Ruuskanen, Matti; Muurinen, Sonja Johanna; Meierjohan, Axel; Pärnänen, Katariina Mariia Margare; Tamminen, Manu Valtteri; Lyra, Christina Darling; Kronberg, Leif ; Virta, Marko Petri Juhani.

In: Journal of Environmental Quality, Vol. 45, No. 2, 2016, p. 488-493.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Fertilizing with Animal Manure Disseminates Antibiotic Resistance Genes to the Farm Environment

AU - Ruuskanen, Matti

AU - Muurinen, Sonja Johanna

AU - Meierjohan, Axel

AU - Pärnänen, Katariina Mariia Margare

AU - Tamminen, Manu Valtteri

AU - Lyra, Christina Darling

AU - Kronberg, Leif

AU - Virta, Marko Petri Juhani

PY - 2016

Y1 - 2016

N2 - The dissemination of antibiotic resistance genes to the environment is an important factor causing increased prevalence of resistant pathogens. Manure is an important fertilizer, but it contains diverse resistance genes. Therefore, its application to fields may lead to increased abundance of resistance genes in the environment. Farming environments exposed to animal manure have not been studied extensively in countries with comparably low antibiotic use, such as Finland. The effects of manure storage and application to fields on the abundance of resistance genes were studied on two dairy cattle farms and two swine farms in southern Finland. Samples were taken from farms during the 2013 cropping season. Copy numbers of carbapenem (blaOXA-58), sulfonamide (sul1), and tetracycline (tetM) resistance genes were measured with quantitative polymerase chain reaction, and the data were analyzed using linear mixed models. The relative abundance of antibiotic resistance genes increased about fourfold in soil after manure application. Carbapenemase encoding blaOXA-58 was detected on all of the studied farms, which indicated that the gene is dispersed in the farm environment. The relative abundance of antibiotic resistance genes increased in stored manure compared with fresh manure roughly fivefold. This study shows that antibiotic resistance genes are disseminated on Finnish production animal farms. The spreading of resistance genes in farm-associated environments could possibly be limited by experimenting with new manure handling methods that could reduce the abundance of the genes in manure used for land application

AB - The dissemination of antibiotic resistance genes to the environment is an important factor causing increased prevalence of resistant pathogens. Manure is an important fertilizer, but it contains diverse resistance genes. Therefore, its application to fields may lead to increased abundance of resistance genes in the environment. Farming environments exposed to animal manure have not been studied extensively in countries with comparably low antibiotic use, such as Finland. The effects of manure storage and application to fields on the abundance of resistance genes were studied on two dairy cattle farms and two swine farms in southern Finland. Samples were taken from farms during the 2013 cropping season. Copy numbers of carbapenem (blaOXA-58), sulfonamide (sul1), and tetracycline (tetM) resistance genes were measured with quantitative polymerase chain reaction, and the data were analyzed using linear mixed models. The relative abundance of antibiotic resistance genes increased about fourfold in soil after manure application. Carbapenemase encoding blaOXA-58 was detected on all of the studied farms, which indicated that the gene is dispersed in the farm environment. The relative abundance of antibiotic resistance genes increased in stored manure compared with fresh manure roughly fivefold. This study shows that antibiotic resistance genes are disseminated on Finnish production animal farms. The spreading of resistance genes in farm-associated environments could possibly be limited by experimenting with new manure handling methods that could reduce the abundance of the genes in manure used for land application

KW - 1172 Environmental sciences

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DO - 10.2134/jeq2015.05.0250

M3 - Article

VL - 45

SP - 488

EP - 493

JO - Journal of Environmental Quality

JF - Journal of Environmental Quality

SN - 0047-2425

IS - 2

ER -