Fetal bovine bone marrow is a rich source of CD34(+) hematopoietic progenitors with myelo-monocytic colony-forming activity

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The CD34 glycoprotein is an important marker of hematopoietic stem cells. We used
a polyclonal rabbit anti-bovine CD34 antibody to stain fetal and adult bovine
bone marrow cells. Flow cytometry revealed a low side scatter (SSC(low))
population of cells that were CD34(+) but negative for leukocyte lineage markers
CD11b, CD14 or CD2. Hematopoietic colony assays with CD34(+) and CD34(-) bone
marrow cells suggested that the colony-forming potential in SSC(low) bone marrow
cells was confined to the CD34(+) fraction. In contrast, this population was not
enriched for cells expressing high aldehyde dehydrogenase activity, a metabolic
marker that has been used to characterize hematopoietic stem cells. Thus, the
CD34 antigen can be used to identify and isolate bovine bone marrow cells
exhibiting clonogenic potential in vitro. Moreover, the proportion of CD34(+)
cells is very high in fetal bovine bone marrow, indicating it as a rich source of
hematopoietic progenitors.
Original languageEnglish
JournalDevelopmental & Comparative Immunology
Volume36
Issue number3
Pages (from-to)572-577
Number of pages6
ISSN0145-305X
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fields of Science

  • Hematopoiesis
  • Hematopoietic stem cell
  • Flow cytometry
  • CD34
  • Fetal bone marrow
  • Cattle
  • Aldehyde dehydrogenase
  • ALDEHYDE DEHYDROGENASE-ACTIVITY
  • HIGH-DOSE CHEMOTHERAPY
  • STEM-CELLS
  • PERIPHERAL-BLOOD
  • MONOCLONAL-ANTIBODY
  • CORD BLOOD
  • STEM/PROGENITOR CELLS
  • MULTIPLE-MYELOMA
  • BREAST-CANCER
  • IN-VIVO
  • 1184 Genetics, developmental biology, physiology
  • 413 Veterinary science

Cite this

@article{3894fc7e1b4d46dc9061c6d2504014e9,
title = "Fetal bovine bone marrow is a rich source of CD34(+) hematopoietic progenitors with myelo-monocytic colony-forming activity",
abstract = "The CD34 glycoprotein is an important marker of hematopoietic stem cells. We useda polyclonal rabbit anti-bovine CD34 antibody to stain fetal and adult bovinebone marrow cells. Flow cytometry revealed a low side scatter (SSC(low))population of cells that were CD34(+) but negative for leukocyte lineage markers CD11b, CD14 or CD2. Hematopoietic colony assays with CD34(+) and CD34(-) bonemarrow cells suggested that the colony-forming potential in SSC(low) bone marrow cells was confined to the CD34(+) fraction. In contrast, this population was not enriched for cells expressing high aldehyde dehydrogenase activity, a metabolicmarker that has been used to characterize hematopoietic stem cells. Thus, theCD34 antigen can be used to identify and isolate bovine bone marrow cellsexhibiting clonogenic potential in vitro. Moreover, the proportion of CD34(+)cells is very high in fetal bovine bone marrow, indicating it as a rich source ofhematopoietic progenitors.",
keywords = "Hematopoiesis, Hematopoietic stem cell, Flow cytometry, CD34, Fetal bone marrow, Cattle, Aldehyde dehydrogenase, ALDEHYDE DEHYDROGENASE-ACTIVITY, HIGH-DOSE CHEMOTHERAPY, STEM-CELLS, PERIPHERAL-BLOOD, MONOCLONAL-ANTIBODY, CORD BLOOD, STEM/PROGENITOR CELLS, MULTIPLE-MYELOMA, BREAST-CANCER, IN-VIVO, 1184 Genetics, developmental biology, physiology, 413 Veterinary science",
author = "Tiina Pessa-Morikawa and Mikael Niku and Antti Iivanainen",
year = "2012",
doi = "10.1016/j.dci.2011.09.014",
language = "English",
volume = "36",
pages = "572--577",
journal = "Developmental & Comparative Immunology",
issn = "0145-305X",
publisher = "PERGAMON",
number = "3",

}

TY - JOUR

T1 - Fetal bovine bone marrow is a rich source of CD34(+) hematopoietic progenitors with myelo-monocytic colony-forming activity

AU - Pessa-Morikawa, Tiina

AU - Niku, Mikael

AU - Iivanainen, Antti

PY - 2012

Y1 - 2012

N2 - The CD34 glycoprotein is an important marker of hematopoietic stem cells. We useda polyclonal rabbit anti-bovine CD34 antibody to stain fetal and adult bovinebone marrow cells. Flow cytometry revealed a low side scatter (SSC(low))population of cells that were CD34(+) but negative for leukocyte lineage markers CD11b, CD14 or CD2. Hematopoietic colony assays with CD34(+) and CD34(-) bonemarrow cells suggested that the colony-forming potential in SSC(low) bone marrow cells was confined to the CD34(+) fraction. In contrast, this population was not enriched for cells expressing high aldehyde dehydrogenase activity, a metabolicmarker that has been used to characterize hematopoietic stem cells. Thus, theCD34 antigen can be used to identify and isolate bovine bone marrow cellsexhibiting clonogenic potential in vitro. Moreover, the proportion of CD34(+)cells is very high in fetal bovine bone marrow, indicating it as a rich source ofhematopoietic progenitors.

AB - The CD34 glycoprotein is an important marker of hematopoietic stem cells. We useda polyclonal rabbit anti-bovine CD34 antibody to stain fetal and adult bovinebone marrow cells. Flow cytometry revealed a low side scatter (SSC(low))population of cells that were CD34(+) but negative for leukocyte lineage markers CD11b, CD14 or CD2. Hematopoietic colony assays with CD34(+) and CD34(-) bonemarrow cells suggested that the colony-forming potential in SSC(low) bone marrow cells was confined to the CD34(+) fraction. In contrast, this population was not enriched for cells expressing high aldehyde dehydrogenase activity, a metabolicmarker that has been used to characterize hematopoietic stem cells. Thus, theCD34 antigen can be used to identify and isolate bovine bone marrow cellsexhibiting clonogenic potential in vitro. Moreover, the proportion of CD34(+)cells is very high in fetal bovine bone marrow, indicating it as a rich source ofhematopoietic progenitors.

KW - Hematopoiesis

KW - Hematopoietic stem cell

KW - Flow cytometry

KW - CD34

KW - Fetal bone marrow

KW - Cattle

KW - Aldehyde dehydrogenase

KW - ALDEHYDE DEHYDROGENASE-ACTIVITY

KW - HIGH-DOSE CHEMOTHERAPY

KW - STEM-CELLS

KW - PERIPHERAL-BLOOD

KW - MONOCLONAL-ANTIBODY

KW - CORD BLOOD

KW - STEM/PROGENITOR CELLS

KW - MULTIPLE-MYELOMA

KW - BREAST-CANCER

KW - IN-VIVO

KW - 1184 Genetics, developmental biology, physiology

KW - 413 Veterinary science

U2 - 10.1016/j.dci.2011.09.014

DO - 10.1016/j.dci.2011.09.014

M3 - Article

VL - 36

SP - 572

EP - 577

JO - Developmental & Comparative Immunology

JF - Developmental & Comparative Immunology

SN - 0145-305X

IS - 3

ER -