Functional diversification of duplicated chalcone synthase genes in anthocyanin biosynthesis of Gerbera hybrida

Xianbao Deng, Hany Bashandy, Miia Ainasoja, Juha Kontturi, Milla Pietiäinen, Roosa Anna Emilia Laitinen, Victor A. Albert, Jari Valkonen, Paula Elomaa, Teemu Teeri

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Chalcone synthase (CHS) is the key enzyme in the first committed step of the flavonoid biosynthetic
pathway and catalyzes the stepwise condensation of 4-coumaroyl-CoA and malonyl-
CoA to naringenin chalcone. In plants, CHS is often encoded by a small family of genes
that are temporally and spatially regulated. Our earlier studies have shown that GCHS4 is
highly activated by ectopic expression of an MYB-type regulator GMYB10 in gerbera
(Gerbera hybrida).
The tissue- and development-specific expression patterns of three gerbera CHS genes were
examined. Virus-induced gene silencing (VIGS) was used to knock down GCHS1 and GCHS4
separately in gerbera inflorescences.
Our data show that GCHS4 is the only CHS encoding gene that is expressed in the cyanidin-
pigmented vegetative tissues of gerbera cv Terraregina. GCHS3 expression is pronounced
in the pappus bristles of the flowers. Expression of both GCHS1 and GCHS4 is high in the epidermal
cells of gerbera petals, but only GCHS1 is contributing to flavonoid biosynthesis.
Gerbera contains a family of three CHS encoding genes showing different spatial and temporal
regulation. GCHS4 expression in gerbera petals is regulated post-transcriptionally, at the
level of either translation elongation or protein stability
Original languageEnglish
JournalNew Phytologist
Volume201
Issue number4
Pages (from-to)1469-1483
Number of pages15
ISSN0028-646X
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 414 Agricultural biotechnology
  • anthocyanin, Asteraceae, chalcone synthase, flavonoid, Gerbera hybrida.

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