Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization

Adiphol Dilokpimol, Miia R. Makela, Gabriella Cerullo, Miaomiao Zhou, Simona Varriale, Loknath Gidijala, Joana L. A. Bras, Peter Jutten, Alexander Piechot, Raymond Verhaert, Vincenza Faraco, Kristiina S. Hilden, Ronald P. de Vries

Research output: Contribution to journalArticleScientificpeer-review

Original languageEnglish
JournalNew Biotechnology
Volume40, Part B
Pages (from-to)282-287
Number of pages6
ISSN1871-6784
DOIs
Publication statusPublished - 25 Jan 2018
MoE publication typeA1 Journal article-refereed
EventThe Congress of the Spanish Federation of Biotechnologists – Annual Congress of Biotechnology - Salamanca, Spain
Duration: 8 Jul 201510 Jul 2015
Conference number: 9

Bibliographical note

4-O-Methyl-d-glucuronic acid (MeGlcA) is a side-residue of glucuronoarabinoxylan and can form ester linkages to lignin, contributing significantly to the strength and rigidity of the plant cell wall. Glucuronoyl esterases (4-O-methyl-glucuronoyl methylesterases, GEs) can cleave this ester bond, and therefore may play a significant role as auxiliary enzymes in biomass saccharification for the production of biofuels and biochemicals. GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy. BLAST analysis with characterized GEs against approximately 250 publicly accessible fungal genomes identified more than 150 putative fungal GEs, which were classified into eight phylogenetic sub-groups. To validate the genome mining strategy, 21 selected GEs from both ascomycete and basidiomycete fungi were heterologously produced in Pichia pastoris. Of these enzymes, 18 were active against benzyl d-glucuronate demonstrating the suitability of our genome mining strategy for enzyme discovery.

Fields of Science

  • Glucuronoyl esterase
  • Glucuronic acid
  • Genome mining
  • Plant cell wall
  • Fungi
  • LIGNIN-CARBOHYDRATE COMPLEXES
  • MYCELIOPHTHORA-THERMOPHILA
  • ACID ESTER
  • MODEL
  • HEMICELLULOSES
  • 416 Food Science

Cite this

Dilokpimol, A., Makela, M. R., Cerullo, G., Zhou, M., Varriale, S., Gidijala, L., ... de Vries, R. P. (2018). Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization. New Biotechnology, 40, Part B, 282-287. https://doi.org/10.1016/j.nbt.2017.10.003
Dilokpimol, Adiphol ; Makela, Miia R. ; Cerullo, Gabriella ; Zhou, Miaomiao ; Varriale, Simona ; Gidijala, Loknath ; Bras, Joana L. A. ; Jutten, Peter ; Piechot, Alexander ; Verhaert, Raymond ; Faraco, Vincenza ; Hilden, Kristiina S. ; de Vries, Ronald P. / Fungal glucuronoyl esterases : Genome mining based enzyme discovery and biochemical characterization. In: New Biotechnology. 2018 ; Vol. 40, Part B. pp. 282-287.
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title = "Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization",
keywords = "Glucuronoyl esterase, Glucuronic acid, Genome mining, Plant cell wall, Fungi, LIGNIN-CARBOHYDRATE COMPLEXES, MYCELIOPHTHORA-THERMOPHILA, ACID ESTER, MODEL, HEMICELLULOSES, 416 Food Science",
author = "Adiphol Dilokpimol and Makela, {Miia R.} and Gabriella Cerullo and Miaomiao Zhou and Simona Varriale and Loknath Gidijala and Bras, {Joana L. A.} and Peter Jutten and Alexander Piechot and Raymond Verhaert and Vincenza Faraco and Hilden, {Kristiina S.} and {de Vries}, {Ronald P.}",
note = "4-O-Methyl-d-glucuronic acid (MeGlcA) is a side-residue of glucuronoarabinoxylan and can form ester linkages to lignin, contributing significantly to the strength and rigidity of the plant cell wall. Glucuronoyl esterases (4-O-methyl-glucuronoyl methylesterases, GEs) can cleave this ester bond, and therefore may play a significant role as auxiliary enzymes in biomass saccharification for the production of biofuels and biochemicals. GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy. BLAST analysis with characterized GEs against approximately 250 publicly accessible fungal genomes identified more than 150 putative fungal GEs, which were classified into eight phylogenetic sub-groups. To validate the genome mining strategy, 21 selected GEs from both ascomycete and basidiomycete fungi were heterologously produced in Pichia pastoris. Of these enzymes, 18 were active against benzyl d-glucuronate demonstrating the suitability of our genome mining strategy for enzyme discovery.",
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Dilokpimol, A, Makela, MR, Cerullo, G, Zhou, M, Varriale, S, Gidijala, L, Bras, JLA, Jutten, P, Piechot, A, Verhaert, R, Faraco, V, Hilden, KS & de Vries, RP 2018, 'Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization' New Biotechnology, vol. 40, Part B, pp. 282-287. https://doi.org/10.1016/j.nbt.2017.10.003

Fungal glucuronoyl esterases : Genome mining based enzyme discovery and biochemical characterization. / Dilokpimol, Adiphol; Makela, Miia R.; Cerullo, Gabriella; Zhou, Miaomiao; Varriale, Simona; Gidijala, Loknath; Bras, Joana L. A.; Jutten, Peter; Piechot, Alexander; Verhaert, Raymond; Faraco, Vincenza; Hilden, Kristiina S.; de Vries, Ronald P.

In: New Biotechnology, Vol. 40, Part B, 25.01.2018, p. 282-287.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Fungal glucuronoyl esterases

T2 - Genome mining based enzyme discovery and biochemical characterization

AU - Dilokpimol, Adiphol

AU - Makela, Miia R.

AU - Cerullo, Gabriella

AU - Zhou, Miaomiao

AU - Varriale, Simona

AU - Gidijala, Loknath

AU - Bras, Joana L. A.

AU - Jutten, Peter

AU - Piechot, Alexander

AU - Verhaert, Raymond

AU - Faraco, Vincenza

AU - Hilden, Kristiina S.

AU - de Vries, Ronald P.

N1 - 4-O-Methyl-d-glucuronic acid (MeGlcA) is a side-residue of glucuronoarabinoxylan and can form ester linkages to lignin, contributing significantly to the strength and rigidity of the plant cell wall. Glucuronoyl esterases (4-O-methyl-glucuronoyl methylesterases, GEs) can cleave this ester bond, and therefore may play a significant role as auxiliary enzymes in biomass saccharification for the production of biofuels and biochemicals. GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy. BLAST analysis with characterized GEs against approximately 250 publicly accessible fungal genomes identified more than 150 putative fungal GEs, which were classified into eight phylogenetic sub-groups. To validate the genome mining strategy, 21 selected GEs from both ascomycete and basidiomycete fungi were heterologously produced in Pichia pastoris. Of these enzymes, 18 were active against benzyl d-glucuronate demonstrating the suitability of our genome mining strategy for enzyme discovery.

PY - 2018/1/25

Y1 - 2018/1/25

KW - Glucuronoyl esterase

KW - Glucuronic acid

KW - Genome mining

KW - Plant cell wall

KW - Fungi

KW - LIGNIN-CARBOHYDRATE COMPLEXES

KW - MYCELIOPHTHORA-THERMOPHILA

KW - ACID ESTER

KW - MODEL

KW - HEMICELLULOSES

KW - 416 Food Science

U2 - 10.1016/j.nbt.2017.10.003

DO - 10.1016/j.nbt.2017.10.003

M3 - Article

VL - 40, Part B

SP - 282

EP - 287

JO - New Biotechnology

JF - New Biotechnology

SN - 1871-6784

ER -