Gene delivery to postnatal rat brain by non-ventricular plasmid injection and electroporation.

Dmitry Molotkov, Alexey Yukin, Ramil Afzalov, Leonard Khirug

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Creation of transgenic animals is a standard approach in studying functions of a gene of interest in vivo. However, many knockout or transgenic animals are not viable in those cases where the modified gene is expressed or deleted in the whole organism. Moreover, a variety of compensatory mechanisms often make it difficult to interpret the results. The compensatory effects can be alleviated by either timing the gene expression or limiting the amount of transfected cells.

The method of postnatal non-ventricular microinjection and in vivo electroporation allows targeted delivery of genes, siRNA or dye molecules directly to a small region of interest in the newborn rodent brain. In contrast to conventional ventricular injection technique, this method allows transfection of non-migratory cell types. Animals transfected by means of the method described here can be used, for example, for two-photon in vivo imaging or in electrophysiological experiments on acute brain slices.
Original languageEnglish
JournalJournal of Visualized Experiments
Issue number43
ISSN1940-087X
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 311 Basic medicine

Cite this

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title = "Gene delivery to postnatal rat brain by non-ventricular plasmid injection and electroporation.",
abstract = "Creation of transgenic animals is a standard approach in studying functions of a gene of interest in vivo. However, many knockout or transgenic animals are not viable in those cases where the modified gene is expressed or deleted in the whole organism. Moreover, a variety of compensatory mechanisms often make it difficult to interpret the results. The compensatory effects can be alleviated by either timing the gene expression or limiting the amount of transfected cells.The method of postnatal non-ventricular microinjection and in vivo electroporation allows targeted delivery of genes, siRNA or dye molecules directly to a small region of interest in the newborn rodent brain. In contrast to conventional ventricular injection technique, this method allows transfection of non-migratory cell types. Animals transfected by means of the method described here can be used, for example, for two-photon in vivo imaging or in electrophysiological experiments on acute brain slices.",
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Gene delivery to postnatal rat brain by non-ventricular plasmid injection and electroporation. / Molotkov, Dmitry; Yukin, Alexey; Afzalov, Ramil; Khirug, Leonard.

In: Journal of Visualized Experiments, No. 43, 2010.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Gene delivery to postnatal rat brain by non-ventricular plasmid injection and electroporation.

AU - Molotkov, Dmitry

AU - Yukin, Alexey

AU - Afzalov, Ramil

AU - Khirug, Leonard

PY - 2010

Y1 - 2010

N2 - Creation of transgenic animals is a standard approach in studying functions of a gene of interest in vivo. However, many knockout or transgenic animals are not viable in those cases where the modified gene is expressed or deleted in the whole organism. Moreover, a variety of compensatory mechanisms often make it difficult to interpret the results. The compensatory effects can be alleviated by either timing the gene expression or limiting the amount of transfected cells.The method of postnatal non-ventricular microinjection and in vivo electroporation allows targeted delivery of genes, siRNA or dye molecules directly to a small region of interest in the newborn rodent brain. In contrast to conventional ventricular injection technique, this method allows transfection of non-migratory cell types. Animals transfected by means of the method described here can be used, for example, for two-photon in vivo imaging or in electrophysiological experiments on acute brain slices.

AB - Creation of transgenic animals is a standard approach in studying functions of a gene of interest in vivo. However, many knockout or transgenic animals are not viable in those cases where the modified gene is expressed or deleted in the whole organism. Moreover, a variety of compensatory mechanisms often make it difficult to interpret the results. The compensatory effects can be alleviated by either timing the gene expression or limiting the amount of transfected cells.The method of postnatal non-ventricular microinjection and in vivo electroporation allows targeted delivery of genes, siRNA or dye molecules directly to a small region of interest in the newborn rodent brain. In contrast to conventional ventricular injection technique, this method allows transfection of non-migratory cell types. Animals transfected by means of the method described here can be used, for example, for two-photon in vivo imaging or in electrophysiological experiments on acute brain slices.

KW - 311 Basic medicine

U2 - 10.3791/2244

DO - 10.3791/2244

M3 - Article

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

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