Genome organization and molecular characterization of the three Formica exsecta viruses—FeV1, FeV2 and FeV4

Research output: Contribution to journalArticleScientificpeer-review

Abstract

We present the genome organization and molecular characterization of the three Formica exsecta viruses, along with ORF predictions, and functional annotation of genes. The Formica exsecta virus-4 (FeV4; GenBank ID: MF287670) is a newly discovered negative-sense single-stranded RNA virus representing the first identified member of order Mononegavirales in ants, whereas the Formica exsecta virus-1 (FeV1; GenBank ID: KF500001), and the Formica exsecta virus-2 (FeV2; GenBank ID: KF500002) are positive single-stranded RNA viruses initially identified (but not characterized) in our earlier study. The new virus FeV4 was found by re-analyzing data from a study published earlier. The Formica exsecta virus-4 genome is 9,866 bp in size, with an overall G + C content of 44.92%, and containing five predicted open reading frames (ORFs). Our bioinformatics analysis indicates that gaps are absent and the ORFs are complete, which based on our comparative genomics analysis suggests that the genomes are complete. Following the characterization, we validate virus infection for FeV1, FeV2 and FeV4 for the first time in field-collected worker ants. Some colonies were infected by multiple viruses, and the viruses were observed to infect all castes, and multiple life stages of workers and queens. Finally, highly similar viruses were expressed in adult workers and queens of six other Formica species: F. fusca, F. pressilabris, F. pratensis, F. aquilonia, F. truncorum and F. cinerea. This research indicates that viruses can be shared between ant species, but further studies on viral transmission are needed to understand viral infection pathways.

Original languageEnglish
Article number6216
JournalPeerJ
Volume7
Issue numbere6216
Number of pages25
ISSN2167-8359
DOIs
Publication statusPublished - 20 Feb 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • ALIGNMENT
  • ANT
  • Comparative analysis
  • DEFORMED WING VIRUS
  • EXPRESSION
  • Formica exsecta
  • Genome
  • HONEY-BEE
  • INFECTIONS
  • Meta-transcriptome
  • Organization and characterization
  • RNA virus
  • SELECTION
  • SEQUENCE
  • TRANSMISSION
  • VARROA
  • 1183 Plant biology, microbiology, virology

Cite this

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title = "Genome organization and molecular characterization of the three Formica exsecta viruses—FeV1, FeV2 and FeV4",
abstract = "We present the genome organization and molecular characterization of the three Formica exsecta viruses, along with ORF predictions, and functional annotation of genes. The Formica exsecta virus-4 (FeV4; GenBank ID: MF287670) is a newly discovered negative-sense single-stranded RNA virus representing the first identified member of order Mononegavirales in ants, whereas the Formica exsecta virus-1 (FeV1; GenBank ID: KF500001), and the Formica exsecta virus-2 (FeV2; GenBank ID: KF500002) are positive single-stranded RNA viruses initially identified (but not characterized) in our earlier study. The new virus FeV4 was found by re-analyzing data from a study published earlier. The Formica exsecta virus-4 genome is 9,866 bp in size, with an overall G + C content of 44.92{\%}, and containing five predicted open reading frames (ORFs). Our bioinformatics analysis indicates that gaps are absent and the ORFs are complete, which based on our comparative genomics analysis suggests that the genomes are complete. Following the characterization, we validate virus infection for FeV1, FeV2 and FeV4 for the first time in field-collected worker ants. Some colonies were infected by multiple viruses, and the viruses were observed to infect all castes, and multiple life stages of workers and queens. Finally, highly similar viruses were expressed in adult workers and queens of six other Formica species: F. fusca, F. pressilabris, F. pratensis, F. aquilonia, F. truncorum and F. cinerea. This research indicates that viruses can be shared between ant species, but further studies on viral transmission are needed to understand viral infection pathways.",
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author = "Dhaygude, {Kishor Uttam} and Helena Johansson and Kulmuni, {Jonna Katharina} and Sundstr{\"o}m, {Liselotte Bj{\"o}rnsdotter}",
year = "2019",
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language = "English",
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Genome organization and molecular characterization of the three Formica exsecta viruses—FeV1, FeV2 and FeV4. / Dhaygude, Kishor Uttam; Johansson, Helena; Kulmuni, Jonna Katharina; Sundström, Liselotte Björnsdotter.

In: PeerJ, Vol. 7, No. e6216, 6216, 20.02.2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Genome organization and molecular characterization of the three Formica exsecta viruses—FeV1, FeV2 and FeV4

AU - Dhaygude, Kishor Uttam

AU - Johansson, Helena

AU - Kulmuni, Jonna Katharina

AU - Sundström, Liselotte Björnsdotter

PY - 2019/2/20

Y1 - 2019/2/20

N2 - We present the genome organization and molecular characterization of the three Formica exsecta viruses, along with ORF predictions, and functional annotation of genes. The Formica exsecta virus-4 (FeV4; GenBank ID: MF287670) is a newly discovered negative-sense single-stranded RNA virus representing the first identified member of order Mononegavirales in ants, whereas the Formica exsecta virus-1 (FeV1; GenBank ID: KF500001), and the Formica exsecta virus-2 (FeV2; GenBank ID: KF500002) are positive single-stranded RNA viruses initially identified (but not characterized) in our earlier study. The new virus FeV4 was found by re-analyzing data from a study published earlier. The Formica exsecta virus-4 genome is 9,866 bp in size, with an overall G + C content of 44.92%, and containing five predicted open reading frames (ORFs). Our bioinformatics analysis indicates that gaps are absent and the ORFs are complete, which based on our comparative genomics analysis suggests that the genomes are complete. Following the characterization, we validate virus infection for FeV1, FeV2 and FeV4 for the first time in field-collected worker ants. Some colonies were infected by multiple viruses, and the viruses were observed to infect all castes, and multiple life stages of workers and queens. Finally, highly similar viruses were expressed in adult workers and queens of six other Formica species: F. fusca, F. pressilabris, F. pratensis, F. aquilonia, F. truncorum and F. cinerea. This research indicates that viruses can be shared between ant species, but further studies on viral transmission are needed to understand viral infection pathways.

AB - We present the genome organization and molecular characterization of the three Formica exsecta viruses, along with ORF predictions, and functional annotation of genes. The Formica exsecta virus-4 (FeV4; GenBank ID: MF287670) is a newly discovered negative-sense single-stranded RNA virus representing the first identified member of order Mononegavirales in ants, whereas the Formica exsecta virus-1 (FeV1; GenBank ID: KF500001), and the Formica exsecta virus-2 (FeV2; GenBank ID: KF500002) are positive single-stranded RNA viruses initially identified (but not characterized) in our earlier study. The new virus FeV4 was found by re-analyzing data from a study published earlier. The Formica exsecta virus-4 genome is 9,866 bp in size, with an overall G + C content of 44.92%, and containing five predicted open reading frames (ORFs). Our bioinformatics analysis indicates that gaps are absent and the ORFs are complete, which based on our comparative genomics analysis suggests that the genomes are complete. Following the characterization, we validate virus infection for FeV1, FeV2 and FeV4 for the first time in field-collected worker ants. Some colonies were infected by multiple viruses, and the viruses were observed to infect all castes, and multiple life stages of workers and queens. Finally, highly similar viruses were expressed in adult workers and queens of six other Formica species: F. fusca, F. pressilabris, F. pratensis, F. aquilonia, F. truncorum and F. cinerea. This research indicates that viruses can be shared between ant species, but further studies on viral transmission are needed to understand viral infection pathways.

KW - ALIGNMENT

KW - ANT

KW - Comparative analysis

KW - DEFORMED WING VIRUS

KW - EXPRESSION

KW - Formica exsecta

KW - Genome

KW - HONEY-BEE

KW - INFECTIONS

KW - Meta-transcriptome

KW - Organization and characterization

KW - RNA virus

KW - SELECTION

KW - SEQUENCE

KW - TRANSMISSION

KW - VARROA

KW - 1183 Plant biology, microbiology, virology

U2 - 10.7717/peerj.6216

DO - 10.7717/peerj.6216

M3 - Article

VL - 7

JO - PeerJ

JF - PeerJ

SN - 2167-8359

IS - e6216

M1 - 6216

ER -