Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls.
Original languageEnglish
JournalInternational Journal of Biological Macromolecules
Volume79
Pages (from-to)807-812
Number of pages6
ISSN0141-8130
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • 116 Chemical sciences

Cite this

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title = "Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent",
abstract = "Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls.",
keywords = "1183 Plant biology, microbiology, virology, 116 Chemical sciences",
author = "Sun-Li Chong and Koutaniemi, {Sanna Maria} and Juvonen, {Minna Katriina} and Marta Derba-Maceluch and Ewa Mellerowicz and Tenkanen, {Tiina Maija}",
year = "2015",
doi = "10.1016/j.ijbiomac.2015.05.056",
language = "English",
volume = "79",
pages = "807--812",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier Scientific Publ. Co",

}

Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent. / Chong, Sun-Li; Koutaniemi, Sanna Maria; Juvonen, Minna Katriina; Derba-Maceluch, Marta; Mellerowicz, Ewa; Tenkanen, Tiina Maija.

In: International Journal of Biological Macromolecules, Vol. 79, 2015, p. 807-812.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent

AU - Chong, Sun-Li

AU - Koutaniemi, Sanna Maria

AU - Juvonen, Minna Katriina

AU - Derba-Maceluch, Marta

AU - Mellerowicz, Ewa

AU - Tenkanen, Tiina Maija

PY - 2015

Y1 - 2015

N2 - Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls.

AB - Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls.

KW - 1183 Plant biology, microbiology, virology

KW - 116 Chemical sciences

U2 - 10.1016/j.ijbiomac.2015.05.056

DO - 10.1016/j.ijbiomac.2015.05.056

M3 - Article

VL - 79

SP - 807

EP - 812

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -