Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent

Sun-Li Chong, Sanna Maria Koutaniemi, Minna Katriina Juvonen, Marta Derba-Maceluch, Ewa Mellerowicz, Tiina Maija Tenkanen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls.
Original languageEnglish
JournalInternational Journal of Biological Macromolecules
Volume79
Pages (from-to)807-812
Number of pages6
ISSN0141-8130
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology
  • 116 Chemical sciences

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