Projects per year
Abstract
More than 2000 authors in alphabetical order.
Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation, it is imperative to target by gene knockout or RNA interference more than one autophagy-related protein. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways implying that not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular assays, we hope to encourage technical innovation in the field.
Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation, it is imperative to target by gene knockout or RNA interference more than one autophagy-related protein. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways implying that not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular assays, we hope to encourage technical innovation in the field.
Original language | English |
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Journal | Autophagy |
Volume | 12 |
Issue number | 1 |
Pages (from-to) | 1-222 |
ISSN | 1554-8627 |
DOIs | |
Publication status | Published - 21 Jan 2016 |
MoE publication type | A1 Journal article-refereed |
Bibliographical note
More than 2000 authors in alphabetical order.Fields of Science
- 3111 Biomedicine
- 1182 Biochemistry, cell and molecular biology
- Autophagy
- phagophore
- amphisome
- autolysosome
- lysosome
Projects
- 2 Finished
-
Dissecting the mammalian phagophore assembly site
Eskelinen, E. & Gudmundsson, S.
European Union, Acedemy of Finland
01/09/2015 → 31/08/2019
Project: Research project
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Three dimensional visualization of autophagosome biogenesis
Eskelinen, E., Biazik, J., Ylä-Anttila, P. & Suntio, T.
01/09/2011 → 31/12/2015
Project: Research project