Heterologous Expression of the Leuconostoc Bacteriocin Leucocin C in Probiotic Yeast Saccharomyces boulardii

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Abstract

The yeastSaccharomyces boulardiiis well known for its probiotic effects such as treating or preventing gastrointestinal diseases. Due to its ability to survive in stomach and intestine,S. boulardiicould be applied as a vehicle for producing and delivering bioactive substances of interest to human gut. In this study, we cloned the genelecCencoding the antilisterial peptide leucocin C from lactic acid bacteriumLeuconostoc carnosuminS. boulardii. The constructedS. boulardiistrain secreted a peptide, which had molecular weight corresponding to leucocin C in SDS-PAGE. The peptide band inhibitedListeria monocytogenesin gel overlay assay. Likewise, concentratedS. boulardiiculture supernatant inhibited the growth ofL. monocytogenes. The growth profile and acid tolerance of the leucocin C secretingS. boulardiiwere similar as those of the strain carrying the empty vector. We further demonstrated that the cells of the leucocin C producingS. boulardiiefficiently killedL. monocytogenes, also without antibiotic selection pressure. These results showed that antilisterial activity could be added to the arsenal of probiotic activities ofS. boulardii, demonstrating its potential as a carrier for therapeutics delivery.

Original languageEnglish
JournalProbiotics and Antimicrobial Proteins
Number of pages9
ISSN1867-1306
DOIs
Publication statusPublished - 21 Jun 2020
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 416 Food Science
  • 11832 Microbiology and virology
  • Probiotic yeast
  • Saccharomyces boulardii
  • Bacteriocin
  • Leucocin C
  • Heterologous expression
  • LACTIC-ACID BACTERIA
  • SACCHAROMYCES-BOULARDII
  • LISTERIA-MONOCYTOGENES
  • CEREVISIAE
  • TRANSLOCATION
  • INTERFERES
  • INHIBITION
  • PROMOTERS
  • EFFICACY
  • DIARRHEA

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