@inbook{b8dadb02ab544251a3f092a28eb35236,
title = "Highly Efficient Production of Dihydroflavonol 4-Reductases in Tobacco Cells and Refinement of the BuOH-HCl Enzymatic Assay",
abstract = "The unintentional escape of the orange petunias from the laboratory to the horticultural market attracted attention although did not cause much safety concern. The unauthorized genetically engineered cultivars were produced by complementing the petunia dihydroflavonol 4-reductase by the corresponding maize enzyme capable of opening the metabolic branch to orange pelargonins. Substrate specificity of dihydroflavonol 4-reductases from different species has been studied for decades and still remains unsolved. Here we show that the enzyme can be efficiently produced in tobacco leaves for enzymatic studies and purification. We refined the classical BuOH-HCl assay for the reductase enzymatic activity into a robust and reliable tool.",
keywords = "1182 Biochemistry, cell and molecular biology, Enzymatic activity, Enzyme Assays, Protein expression, Dihydroflavonol 4-reductase, ANTHOCYANIN BIOSYNTHESIS, 11831 Plant biology, Tobacco, Nicotiana benthamiana, Agrobacterium tumefaciens-mediated transformation, Biotechnology, Petunia hybrida, Maize, 116 Chemical sciences, HPLC, HPLC analysis, Analytical chemistry, Polyphenol, Anthocyanin",
author = "Lingping Zhu and Saku Mattila and Roosa Matom{\"a}ki and Lorenzo Mollo and Sharmin Ahamed and Abdou, {Sara M.} and Bashandy, {Hany Said Mohamed El Sayed} and Teemu Teeri",
year = "2023",
month = feb,
day = "3",
doi = "10.1002/9781119844792.ch9",
language = "English",
isbn = "978-1-119-84476-1",
volume = "8",
series = "Recent Advances in Polyphenol Research",
publisher = "John Wiley & Sons Ltd. ",
pages = "281--300",
booktitle = "Recent Advances in Polyphenol Research",
address = "United States",
}