Host response in bovine mastitis experimentally induced with Staphylococcus chromogenes

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    "An experimental infection model was developed to study host response to intramammary infection in cows caused by Staphylococcus chromogenes. CNS intramammary infections have become very common in modern dairy herds, and they can remain persistent in the mammary gland. More information would be needed about the pathophysiology of CNS mastitis, and an experimental mastitis model is a means for this research. Six primiparous Holstein-Friesian cows were challenged with S. chromogenes; 4 weeks after calving, One udder quarter of each cow was inoculated with 2.1 x 10(6) cfu of S. chromogenes. All cows became infected and clinical signs were mild. Milk production of the challenged quarter decreased on average by 16.3% during 7 days post-challenge. Cows eliminated bacteria in a few days, except for one cow which developed persistent mastitis. Milk indicators of inflammation, SCC and N-acetyl-beta-D-glucosaminidase (NAGase) returned to normal within a week. Milk NAGase activity increased moderately, which reflects minor tissue damage in the udder. Concentrations of serum amyloid A (SAA) and milk amyloid A (MAA) were both elevated at 12 h PC. MAA was affected by the milking times, and was at its highest before the morning milking. In our experimental model, systemic acute phase protein response with SAA occurred as an on-off type reaction. In conclusion, this experimental model could be used to study host response in CNS mastitis caused by the main CNS species and also for comparison of the host response in a mild intramammary infection and in more severe mastitis models. (C) 2008 Elsevier B.V. All rights reserved."
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume134
    Issue number1/2
    Pages (from-to)95-99
    Number of pages5
    ISSN0378-1135
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • Bovine
    • Mastitis
    • Coagulase-negative staphylococci
    • Experimental mastitis model
    • Serum amyloid A
    • Milk amyloid A
    • COAGULASE-NEGATIVE STAPHYLOCOCCI
    • SERUM-AMYLOID-A
    • MAMMARY EPITHELIAL-CELLS
    • BETA-D-GLUCOSAMINIDASE
    • ACUTE-PHASE RESPONSE
    • DAIRY-COWS
    • INTRAMAMMARY INFECTIONS
    • MILK
    • PATHOGENESIS
    • EPIDERMIDIS
    • 413 Veterinary science

    Cite this

    @article{75cb285d6f7549aeb01c8dc339db6b95,
    title = "Host response in bovine mastitis experimentally induced with Staphylococcus chromogenes",
    abstract = "{"}An experimental infection model was developed to study host response to intramammary infection in cows caused by Staphylococcus chromogenes. CNS intramammary infections have become very common in modern dairy herds, and they can remain persistent in the mammary gland. More information would be needed about the pathophysiology of CNS mastitis, and an experimental mastitis model is a means for this research. Six primiparous Holstein-Friesian cows were challenged with S. chromogenes; 4 weeks after calving, One udder quarter of each cow was inoculated with 2.1 x 10(6) cfu of S. chromogenes. All cows became infected and clinical signs were mild. Milk production of the challenged quarter decreased on average by 16.3{\%} during 7 days post-challenge. Cows eliminated bacteria in a few days, except for one cow which developed persistent mastitis. Milk indicators of inflammation, SCC and N-acetyl-beta-D-glucosaminidase (NAGase) returned to normal within a week. Milk NAGase activity increased moderately, which reflects minor tissue damage in the udder. Concentrations of serum amyloid A (SAA) and milk amyloid A (MAA) were both elevated at 12 h PC. MAA was affected by the milking times, and was at its highest before the morning milking. In our experimental model, systemic acute phase protein response with SAA occurred as an on-off type reaction. In conclusion, this experimental model could be used to study host response in CNS mastitis caused by the main CNS species and also for comparison of the host response in a mild intramammary infection and in more severe mastitis models. (C) 2008 Elsevier B.V. All rights reserved.{"}",
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    author = "H. Simojoki and T. Orro and S. Taponen and S. Py{\"o}r{\"a}l{\"a}",
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    doi = "10.1016/j.vetmic.2008.09.003",
    language = "English",
    volume = "134",
    pages = "95--99",
    journal = "Veterinary Microbiology",
    issn = "0378-1135",
    publisher = "Elsevier Scientific Publ. Co",
    number = "1/2",

    }

    Host response in bovine mastitis experimentally induced with Staphylococcus chromogenes. / Simojoki, H.; Orro, T.; Taponen, S.; Pyörälä, S.

    In: Veterinary Microbiology, Vol. 134, No. 1/2, 2009, p. 95-99.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Host response in bovine mastitis experimentally induced with Staphylococcus chromogenes

    AU - Simojoki, H.

    AU - Orro, T.

    AU - Taponen, S.

    AU - Pyörälä, S.

    PY - 2009

    Y1 - 2009

    N2 - "An experimental infection model was developed to study host response to intramammary infection in cows caused by Staphylococcus chromogenes. CNS intramammary infections have become very common in modern dairy herds, and they can remain persistent in the mammary gland. More information would be needed about the pathophysiology of CNS mastitis, and an experimental mastitis model is a means for this research. Six primiparous Holstein-Friesian cows were challenged with S. chromogenes; 4 weeks after calving, One udder quarter of each cow was inoculated with 2.1 x 10(6) cfu of S. chromogenes. All cows became infected and clinical signs were mild. Milk production of the challenged quarter decreased on average by 16.3% during 7 days post-challenge. Cows eliminated bacteria in a few days, except for one cow which developed persistent mastitis. Milk indicators of inflammation, SCC and N-acetyl-beta-D-glucosaminidase (NAGase) returned to normal within a week. Milk NAGase activity increased moderately, which reflects minor tissue damage in the udder. Concentrations of serum amyloid A (SAA) and milk amyloid A (MAA) were both elevated at 12 h PC. MAA was affected by the milking times, and was at its highest before the morning milking. In our experimental model, systemic acute phase protein response with SAA occurred as an on-off type reaction. In conclusion, this experimental model could be used to study host response in CNS mastitis caused by the main CNS species and also for comparison of the host response in a mild intramammary infection and in more severe mastitis models. (C) 2008 Elsevier B.V. All rights reserved."

    AB - "An experimental infection model was developed to study host response to intramammary infection in cows caused by Staphylococcus chromogenes. CNS intramammary infections have become very common in modern dairy herds, and they can remain persistent in the mammary gland. More information would be needed about the pathophysiology of CNS mastitis, and an experimental mastitis model is a means for this research. Six primiparous Holstein-Friesian cows were challenged with S. chromogenes; 4 weeks after calving, One udder quarter of each cow was inoculated with 2.1 x 10(6) cfu of S. chromogenes. All cows became infected and clinical signs were mild. Milk production of the challenged quarter decreased on average by 16.3% during 7 days post-challenge. Cows eliminated bacteria in a few days, except for one cow which developed persistent mastitis. Milk indicators of inflammation, SCC and N-acetyl-beta-D-glucosaminidase (NAGase) returned to normal within a week. Milk NAGase activity increased moderately, which reflects minor tissue damage in the udder. Concentrations of serum amyloid A (SAA) and milk amyloid A (MAA) were both elevated at 12 h PC. MAA was affected by the milking times, and was at its highest before the morning milking. In our experimental model, systemic acute phase protein response with SAA occurred as an on-off type reaction. In conclusion, this experimental model could be used to study host response in CNS mastitis caused by the main CNS species and also for comparison of the host response in a mild intramammary infection and in more severe mastitis models. (C) 2008 Elsevier B.V. All rights reserved."

    KW - Bovine

    KW - Mastitis

    KW - Coagulase-negative staphylococci

    KW - Experimental mastitis model

    KW - Serum amyloid A

    KW - Milk amyloid A

    KW - COAGULASE-NEGATIVE STAPHYLOCOCCI

    KW - SERUM-AMYLOID-A

    KW - MAMMARY EPITHELIAL-CELLS

    KW - BETA-D-GLUCOSAMINIDASE

    KW - ACUTE-PHASE RESPONSE

    KW - DAIRY-COWS

    KW - INTRAMAMMARY INFECTIONS

    KW - MILK

    KW - PATHOGENESIS

    KW - EPIDERMIDIS

    KW - 413 Veterinary science

    U2 - 10.1016/j.vetmic.2008.09.003

    DO - 10.1016/j.vetmic.2008.09.003

    M3 - Article

    VL - 134

    SP - 95

    EP - 99

    JO - Veterinary Microbiology

    JF - Veterinary Microbiology

    SN - 0378-1135

    IS - 1/2

    ER -