HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer

Laura Pietola, Antti Aarnisalo, Joonas Joensuu, Riikka Pellinen, Jarmo Wahlroos, Jussi Jero

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    "Conclusion. GFP transgene was expressed in the lining cells of the perilymphatic space. Lentivirus vectors are safe and cause only minimal inflammatory reaction. Transgene products can be delivered into the perilymph by utilizing lentivirus vectors. Objectives. To analyze the efficiency and safety of lentiviral vectors HOX-GFP and WOX-GFP in intracochlear gene transfer. Materials and methods. Lentivirus vectors were tested for their transduction efficiency in vivo in CD-1 mice. Half of the animals were pretreated with kanamycin. Lentivirus vector or saline (1 mu l) was injected into the inner car. All. the animals were sacrificed 14 days after the surgery and the cochleae and selected organs were analyzed immunohistochemically. Results. HOX-GFP and WOX-GFP expression was restricted to the lining cells of the scala tympani and scala vestibuli. No GFP expression was seen in the organ of Corti or the spiral ganglion. Aminoglycoside treatment had no effect on the expression of these vectors. The distant spread of lentivirus vectors was minimal; only the liver of one animal showed some GFP expression. Inflammatory reaction caused by these vectors was mild. Few inflammatory cells were found in the perilymphatic space of the cochlea and in the vestibular organ."
    Original languageEnglish
    JournalActa Oto-Laryngologica
    Volume128
    Issue number6
    Pages (from-to)613-620
    Number of pages8
    ISSN0001-6489
    DOIs
    Publication statusPublished - 2008
    MoE publication typeA1 Journal article-refereed

    Cite this

    Pietola, Laura ; Aarnisalo, Antti ; Joensuu, Joonas ; Pellinen, Riikka ; Wahlroos, Jarmo ; Jero, Jussi. / HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer. In: Acta Oto-Laryngologica. 2008 ; Vol. 128, No. 6. pp. 613-620.
    @article{914de860196d4864890e285f4136e9fe,
    title = "HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer",
    abstract = "{"}Conclusion. GFP transgene was expressed in the lining cells of the perilymphatic space. Lentivirus vectors are safe and cause only minimal inflammatory reaction. Transgene products can be delivered into the perilymph by utilizing lentivirus vectors. Objectives. To analyze the efficiency and safety of lentiviral vectors HOX-GFP and WOX-GFP in intracochlear gene transfer. Materials and methods. Lentivirus vectors were tested for their transduction efficiency in vivo in CD-1 mice. Half of the animals were pretreated with kanamycin. Lentivirus vector or saline (1 mu l) was injected into the inner car. All. the animals were sacrificed 14 days after the surgery and the cochleae and selected organs were analyzed immunohistochemically. Results. HOX-GFP and WOX-GFP expression was restricted to the lining cells of the scala tympani and scala vestibuli. No GFP expression was seen in the organ of Corti or the spiral ganglion. Aminoglycoside treatment had no effect on the expression of these vectors. The distant spread of lentivirus vectors was minimal; only the liver of one animal showed some GFP expression. Inflammatory reaction caused by these vectors was mild. Few inflammatory cells were found in the perilymphatic space of the cochlea and in the vestibular organ.{"}",
    author = "Laura Pietola and Antti Aarnisalo and Joonas Joensuu and Riikka Pellinen and Jarmo Wahlroos and Jussi Jero",
    year = "2008",
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    language = "English",
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    HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer. / Pietola, Laura; Aarnisalo, Antti; Joensuu, Joonas; Pellinen, Riikka; Wahlroos, Jarmo; Jero, Jussi.

    In: Acta Oto-Laryngologica, Vol. 128, No. 6, 2008, p. 613-620.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer

    AU - Pietola, Laura

    AU - Aarnisalo, Antti

    AU - Joensuu, Joonas

    AU - Pellinen, Riikka

    AU - Wahlroos, Jarmo

    AU - Jero, Jussi

    PY - 2008

    Y1 - 2008

    N2 - "Conclusion. GFP transgene was expressed in the lining cells of the perilymphatic space. Lentivirus vectors are safe and cause only minimal inflammatory reaction. Transgene products can be delivered into the perilymph by utilizing lentivirus vectors. Objectives. To analyze the efficiency and safety of lentiviral vectors HOX-GFP and WOX-GFP in intracochlear gene transfer. Materials and methods. Lentivirus vectors were tested for their transduction efficiency in vivo in CD-1 mice. Half of the animals were pretreated with kanamycin. Lentivirus vector or saline (1 mu l) was injected into the inner car. All. the animals were sacrificed 14 days after the surgery and the cochleae and selected organs were analyzed immunohistochemically. Results. HOX-GFP and WOX-GFP expression was restricted to the lining cells of the scala tympani and scala vestibuli. No GFP expression was seen in the organ of Corti or the spiral ganglion. Aminoglycoside treatment had no effect on the expression of these vectors. The distant spread of lentivirus vectors was minimal; only the liver of one animal showed some GFP expression. Inflammatory reaction caused by these vectors was mild. Few inflammatory cells were found in the perilymphatic space of the cochlea and in the vestibular organ."

    AB - "Conclusion. GFP transgene was expressed in the lining cells of the perilymphatic space. Lentivirus vectors are safe and cause only minimal inflammatory reaction. Transgene products can be delivered into the perilymph by utilizing lentivirus vectors. Objectives. To analyze the efficiency and safety of lentiviral vectors HOX-GFP and WOX-GFP in intracochlear gene transfer. Materials and methods. Lentivirus vectors were tested for their transduction efficiency in vivo in CD-1 mice. Half of the animals were pretreated with kanamycin. Lentivirus vector or saline (1 mu l) was injected into the inner car. All. the animals were sacrificed 14 days after the surgery and the cochleae and selected organs were analyzed immunohistochemically. Results. HOX-GFP and WOX-GFP expression was restricted to the lining cells of the scala tympani and scala vestibuli. No GFP expression was seen in the organ of Corti or the spiral ganglion. Aminoglycoside treatment had no effect on the expression of these vectors. The distant spread of lentivirus vectors was minimal; only the liver of one animal showed some GFP expression. Inflammatory reaction caused by these vectors was mild. Few inflammatory cells were found in the perilymphatic space of the cochlea and in the vestibular organ."

    U2 - 10.1080/00016480701663409

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    M3 - Article

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    EP - 620

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