Human mast cells arise from a common circulating progenitor

Research output: Contribution to journalArticleScientificpeer-review

Abstract

BACKGROUND: Human tissue mast cells (MCs) have the potential to express several neutral granule proteases, which are the most precise markers of the phenotypic heterogeneity of MCs. However, the full extent of such heterogeneity is limited by the fact that MCs containing either tryptase only or tryptase and chymase have long been considered to be the sole MC phenotypes. Moreover, the potential developmental relationship between human MCs of different protease phenotypes has remained a matter of dispute.
OBJECTIVE: We attempted to define how human MCs with different protease phenotypes relate to their circulating progenitors.
METHODS: MCs were generated from human peripheral blood-derived CD34(+) progenitors in the presence of kit ligand (KITLG) and the cytokines IL-3, IL-9, and IL-6 under serum-free conditions, or by KITLG alone in the presence or absence of serum. The expression of chymase, carboxypeptidase A3, cathepsin G, granzyme B, and the tryptases derived from the TPSAB1, TPSB2, TPSD1, and TPSG1/PRSS31 genes were determined weekly at the mRNA and/or protein levels.
RESULTS: Incubation of CD34(+) progenitors in the presence of KITLG and the cytokines IL-3, IL-9, and IL-6 promoted the development of a single population of MCs with a uniform tryptase(+), chymase(+), CPA3(+), cathepsin G(+), and granzyme B(+) phenotype. Interestingly, the presence of KITLG alone was sufficient to induce the expression of all the above proteases.
CONCLUSION: All circulating human MC progenitors have the potential to differentiate into MCs expressing the complete panel of neutral granule proteases, implying that human MCs originate from a common MC-committed progenitor.
Original languageEnglish
JournalJournal of Allergy and Clinical Immunology
Pages (from-to)463-469
Number of pages8
ISSN0091-6749
DOIs
Publication statusPublished - Aug 2013
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1182 Biochemistry, cell and molecular biology
  • Mast Cells
  • Hematopoietic progenitors
  • Proteases
  • Cell differentiation
  • Immunology
  • Chymase
  • Tryptase
  • Cathepsin G
  • Granzyme B
  • Carboxypeptidase A3
  • Stem Cell Factor

Cite this

@article{e551df42b36f48e496929e9ac4479fcf,
title = "Human mast cells arise from a common circulating progenitor",
abstract = "BACKGROUND: Human tissue mast cells (MCs) have the potential to express several neutral granule proteases, which are the most precise markers of the phenotypic heterogeneity of MCs. However, the full extent of such heterogeneity is limited by the fact that MCs containing either tryptase only or tryptase and chymase have long been considered to be the sole MC phenotypes. Moreover, the potential developmental relationship between human MCs of different protease phenotypes has remained a matter of dispute.OBJECTIVE: We attempted to define how human MCs with different protease phenotypes relate to their circulating progenitors.METHODS: MCs were generated from human peripheral blood-derived CD34(+) progenitors in the presence of kit ligand (KITLG) and the cytokines IL-3, IL-9, and IL-6 under serum-free conditions, or by KITLG alone in the presence or absence of serum. The expression of chymase, carboxypeptidase A3, cathepsin G, granzyme B, and the tryptases derived from the TPSAB1, TPSB2, TPSD1, and TPSG1/PRSS31 genes were determined weekly at the mRNA and/or protein levels.RESULTS: Incubation of CD34(+) progenitors in the presence of KITLG and the cytokines IL-3, IL-9, and IL-6 promoted the development of a single population of MCs with a uniform tryptase(+), chymase(+), CPA3(+), cathepsin G(+), and granzyme B(+) phenotype. Interestingly, the presence of KITLG alone was sufficient to induce the expression of all the above proteases.CONCLUSION: All circulating human MC progenitors have the potential to differentiate into MCs expressing the complete panel of neutral granule proteases, implying that human MCs originate from a common MC-committed progenitor.",
keywords = "1182 Biochemistry, cell and molecular biology, Mast Cells, Hematopoietic progenitors, Mast Cells, Hematopoietic progenitors, Proteases, Cell differentiation, Immunology, Chymase, Tryptase, Cathepsin G, Granzyme B, Carboxypeptidase A3, Stem Cell Factor",
author = "Katariina Maaninka and Jani Lappalainen and Kovanen, {Petri T}",
year = "2013",
month = "8",
doi = "10.1016/j.jaci.2013.02.011",
language = "English",
pages = "463--469",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Elsevier",

}

Human mast cells arise from a common circulating progenitor. / Maaninka, Katariina; Lappalainen, Jani; Kovanen, Petri T.

In: Journal of Allergy and Clinical Immunology, 08.2013, p. 463-469.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Human mast cells arise from a common circulating progenitor

AU - Maaninka, Katariina

AU - Lappalainen, Jani

AU - Kovanen, Petri T

PY - 2013/8

Y1 - 2013/8

N2 - BACKGROUND: Human tissue mast cells (MCs) have the potential to express several neutral granule proteases, which are the most precise markers of the phenotypic heterogeneity of MCs. However, the full extent of such heterogeneity is limited by the fact that MCs containing either tryptase only or tryptase and chymase have long been considered to be the sole MC phenotypes. Moreover, the potential developmental relationship between human MCs of different protease phenotypes has remained a matter of dispute.OBJECTIVE: We attempted to define how human MCs with different protease phenotypes relate to their circulating progenitors.METHODS: MCs were generated from human peripheral blood-derived CD34(+) progenitors in the presence of kit ligand (KITLG) and the cytokines IL-3, IL-9, and IL-6 under serum-free conditions, or by KITLG alone in the presence or absence of serum. The expression of chymase, carboxypeptidase A3, cathepsin G, granzyme B, and the tryptases derived from the TPSAB1, TPSB2, TPSD1, and TPSG1/PRSS31 genes were determined weekly at the mRNA and/or protein levels.RESULTS: Incubation of CD34(+) progenitors in the presence of KITLG and the cytokines IL-3, IL-9, and IL-6 promoted the development of a single population of MCs with a uniform tryptase(+), chymase(+), CPA3(+), cathepsin G(+), and granzyme B(+) phenotype. Interestingly, the presence of KITLG alone was sufficient to induce the expression of all the above proteases.CONCLUSION: All circulating human MC progenitors have the potential to differentiate into MCs expressing the complete panel of neutral granule proteases, implying that human MCs originate from a common MC-committed progenitor.

AB - BACKGROUND: Human tissue mast cells (MCs) have the potential to express several neutral granule proteases, which are the most precise markers of the phenotypic heterogeneity of MCs. However, the full extent of such heterogeneity is limited by the fact that MCs containing either tryptase only or tryptase and chymase have long been considered to be the sole MC phenotypes. Moreover, the potential developmental relationship between human MCs of different protease phenotypes has remained a matter of dispute.OBJECTIVE: We attempted to define how human MCs with different protease phenotypes relate to their circulating progenitors.METHODS: MCs were generated from human peripheral blood-derived CD34(+) progenitors in the presence of kit ligand (KITLG) and the cytokines IL-3, IL-9, and IL-6 under serum-free conditions, or by KITLG alone in the presence or absence of serum. The expression of chymase, carboxypeptidase A3, cathepsin G, granzyme B, and the tryptases derived from the TPSAB1, TPSB2, TPSD1, and TPSG1/PRSS31 genes were determined weekly at the mRNA and/or protein levels.RESULTS: Incubation of CD34(+) progenitors in the presence of KITLG and the cytokines IL-3, IL-9, and IL-6 promoted the development of a single population of MCs with a uniform tryptase(+), chymase(+), CPA3(+), cathepsin G(+), and granzyme B(+) phenotype. Interestingly, the presence of KITLG alone was sufficient to induce the expression of all the above proteases.CONCLUSION: All circulating human MC progenitors have the potential to differentiate into MCs expressing the complete panel of neutral granule proteases, implying that human MCs originate from a common MC-committed progenitor.

KW - 1182 Biochemistry, cell and molecular biology

KW - Mast Cells

KW - Hematopoietic progenitors

KW - Mast Cells

KW - Hematopoietic progenitors

KW - Proteases

KW - Cell differentiation

KW - Immunology

KW - Chymase

KW - Tryptase

KW - Cathepsin G

KW - Granzyme B

KW - Carboxypeptidase A3

KW - Stem Cell Factor

U2 - 10.1016/j.jaci.2013.02.011

DO - 10.1016/j.jaci.2013.02.011

M3 - Article

SP - 463

EP - 469

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

ER -