Projects per year
Abstract
Fungal decomposition of wood is considered as a strictly aerobic process. However, recent findings on wood-decaying fungi to produce ethanol from various lignocelluloses under oxygen-depleted conditions lead us to question this. We designed gene expression study of the white rot fungus Phlebia radiata (isolate FBCC0043) by adopting comparative transcriptomics and functional genomics on solid lignocellulose substrates under varying cultivation atmospheric conditions.
ResultsSwitch to fermentative conditions was a major regulator for intracellular metabolism and extracellular enzymatic degradation of wood polysaccharides. Changes in the expression profiles of CAZy (carbohydrate-active enzyme) encoding genes upon oxygen depletion, lead into an alternative wood decomposition strategy. Surprisingly, we noticed higher cellulolytic activity under fermentative conditions in comparison to aerobic cultivation. In addition, our results manifest how oxygen depletion affects over 200 genes of fungal primary metabolism including several transcription factors. We present new functions for acetate generating phosphoketolase pathway and its potential regulator, Adr1 transcription factor, in carbon catabolism under oxygen depletion.
ConclusionsPhysiologically resilient wood-decomposing Basidiomycota species P. radiata is capable of thriving under respirative and fermentative conditions utilizing only untreated lignocellulose as carbon source. Hypoxia-response mechanism in the fungus is, however, divergent from the regulation described for Ascomycota fermenting yeasts or animal-pathogenic species of Basidiomycota.
Original language | English |
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Article number | 26 |
Journal | Biotechnology for Biofuels |
Volume | 13 |
Issue number | 1 |
Number of pages | 17 |
ISSN | 1754-6834 |
DOIs | |
Publication status | Published - 24 Feb 2020 |
MoE publication type | A1 Journal article-refereed |
Fields of Science
- 11832 Microbiology and virology
- 220 Industrial biotechnology
Projects
- 4 Finished
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Biofungi - kääpäsienten fermentaatiokyvyn ja aineenvaihdunnan reittien avaus jätteiden muuntamisessa
Lundell, T., Kiviniemi, E. A., Mattila, H., Christodoulou, M., Hurtado López, N., Kiviniemi, E. A., Laine, T. A. K., Mali, T., Mattila, H., Mikkola, A. H., Pulkkanen, R. E. & Österman-Udd, J.
01/01/2019 → 31/08/2022
Project: Foundations (Private Foundations, Non-Profit Foundations, Charitable Trusts)
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Fungcolife: Fungal interactions and life-styles on wood
Lundell, T., Mali, T., Mattila, H., Shah, F. & Mäkinen, M.
01/09/2015 → 31/12/2019
Project: Research project
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Fungal degradation and conversion of recyclable lignocelluloses
01/06/2015 → 31/12/2018
Project: Research project
Equipment
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HAMBI Microbial Culture Collection, University of Helsinki
Pekka Oivanen (Operator) & Kaarina Sivonen (Manager)
Department of MicrobiologyFacility/equipment: Equipment
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Instruct-HiLIFE Biomolecular Complex Purification Core Facility (Biocomplex)
Minna Marjetta Poranen (Manager), Hanna Maarit Oksanen (Other) & Katri Johanna Eskelin (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Core Facility
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Natural organic compounds analysis unit
Department of Food and NutritionFacility/equipment: Equipment