Identification of cambium stem cell factors and their positioning mechanism

Gugan Eswaran; Xixi Zhang; Jacob Pieter Rutten; Jingyi Han; Hiroyuki Iida; Jennifer López Ortiz; Riikka Mäkilä; Brecht Wybouw; Benjamin Planterose Jiménez; Leo Vainio; Alexis Porcher; Marina Leal Gavarron; Jing Zhang; Tiina Blomster; Xin Wang; David Dolan; Ondřej Smetana; Siobhán M. Brady; Melis Kucukoglu Topcu; Kirsten ten Tusscher; J. Peter Etchells; Ari Pekka Mähönen

doi:10.1126/science.adj8752

Identification of cambium stem cell factors and their positioning mechanism

Gugan Eswaran, Xixi Zhang, Jacob Pieter Rutten, Jingyi Han, Hiroyuki Iida, Jennifer López Ortiz, Riikka Mäkilä, Brecht Wybouw, Benjamin Planterose Jiménez, Leo Vainio, Alexis Porcher, Marina Leal Gavarron, Jing Zhang, Tiina Blomster, Xin Wang, David Dolan, Ondřej Smetana, Siobhán M. Brady, Melis Kucukoglu Topcu, Kirsten ten TusscherJ. Peter Etchells, Ari Pekka Mähönen

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand–activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

Original language	English
Journal	Science
Volume	386
Issue number	6722
Pages (from-to)	646-653
Number of pages	8
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.adj8752
Publication status	Published - 8 Nov 2024
MoE publication type	A1 Journal article-refereed

Bibliographical note

Publisher Copyright:
© 2024 American Association for the Advancement of Science. All rights reserved.

Fields of Science

11831 Plant biology
Arabidopsis root
Secondary growth
Wood formation
Cle peptides
Gene
Diffusion
Aintegumenta
Proteins
Wall
Pxy

Access to Document

10.1126/science.adj8752

2024-09-20_Eswaran_et_al_V70_Accepted_VersionAccepted author manuscript, 4.46 MB

Cite this

Eswaran, G., Zhang, X., Rutten, J. P., Han, J., Iida, H., López Ortiz, J., Mäkilä, R., Wybouw, B., Jiménez, B. P., Vainio, L., Porcher, A., Gavarron, M. L., Zhang, J., Blomster, T., Wang, X., Dolan, D., Smetana, O., Brady, S. M., Topcu, M. K., ... Mähönen, A. P. (2024). Identification of cambium stem cell factors and their positioning mechanism. Science, 386(6722), 646-653. https://doi.org/10.1126/science.adj8752

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abstract = "Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand–activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.",

keywords = "11831 Plant biology, Arabidopsis root, Secondary growth, Wood formation, Cle peptides, Gene, Diffusion, Aintegumenta, Proteins, Wall, Pxy",

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Eswaran, G , Zhang, X, Rutten, JP, Han, J, Iida, H , López Ortiz, J , Mäkilä, R , Wybouw, B, Jiménez, BP, Vainio, L , Porcher, A , Gavarron, ML, Zhang, J, Blomster, T , Wang, X, Dolan, D, Smetana, O, Brady, SM, Topcu, MK, Tusscher, KT, Etchells, JP & Mähönen, AP 2024, 'Identification of cambium stem cell factors and their positioning mechanism', Science, vol. 386, no. 6722, pp. 646-653. https://doi.org/10.1126/science.adj8752

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T1 - Identification of cambium stem cell factors and their positioning mechanism

AU - Eswaran, Gugan

AU - Zhang, Xixi

AU - Rutten, Jacob Pieter

AU - Han, Jingyi

AU - Iida, Hiroyuki

AU - López Ortiz, Jennifer

AU - Mäkilä, Riikka

AU - Wybouw, Brecht

AU - Jiménez, Benjamin Planterose

AU - Vainio, Leo

AU - Porcher, Alexis

AU - Gavarron, Marina Leal

AU - Zhang, Jing

AU - Blomster, Tiina

AU - Wang, Xin

AU - Dolan, David

AU - Smetana, Ondřej

AU - Brady, Siobhán M.

AU - Topcu, Melis Kucukoglu

AU - Tusscher, Kirsten ten

AU - Etchells, J. Peter

AU - Mähönen, Ari Pekka

PY - 2024/11/8

Y1 - 2024/11/8

N2 - Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand–activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

AB - Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand–activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

KW - 11831 Plant biology

KW - Arabidopsis root

KW - Secondary growth

KW - Wood formation

KW - Cle peptides

KW - Gene

KW - Diffusion

KW - Aintegumenta

KW - Proteins

KW - Wall

KW - Pxy

U2 - 10.1126/science.adj8752

DO - 10.1126/science.adj8752

M3 - Article

C2 - 39509505

AN - SCOPUS:85209165432

SN - 0036-8075

VL - 386

SP - 646

EP - 653

JO - Science

JF - Science

IS - 6722

ER -