Abstract
Neuronal histamine regulates several functions in the vertebrate brain. The zebrafish brain contains a widespread histaminergic system and H-3 receptor ligand binding has been reported. In this study we provide evidence for the existence of histamine H-1, H-2 and H-3 receptor genes in zebrafish. Single copies of putative histamine H-1, H-2 and H-3 receptors were identified and cloned from the zebrafish brain. Expression analysis suggested that they are expressed in the brain and a few other tissues. Widespread distribution of zebrafish H2 receptor binding sites was detected with [I-125]iodoaminopotentidine in brain sections. Zebrafish larvae were exposed to 1, 10 or 100 mu M of the H-1 ligand pyrilamine, the H-2 ligand cimetidine and the H-3 ligands thioperamide and immepip for 5 days. Significant decreases in swimming distance were observed with the highest dose of all ligands, whereas cimetidine gave a significant decrease also with 1 and 10 mu M doses. These results provide the first molecular biological evidence for the presence of histamine receptors in zebrafish. These histamine receptors resemble those of higher vertebrates and they provide a useful model for pharmacological and behavioral studies for characterizing the functions of histamine in more detail. (c) 2007 Elsevier Inc. All rights reserved.
Original language | English |
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Journal | Biochemical Pharmacology |
Volume | 73 |
Pages (from-to) | 1205-1214 |
Number of pages | 10 |
ISSN | 0006-2952 |
DOIs | |
Publication status | Published - 2007 |
MoE publication type | A1 Journal article-refereed |
Fields of Science
- 311 Basic medicine
- 118 Biological sciences
- 515 Psychology