Listeria monocytogenes in ready-to-eat vacuum and modified atmosphere packaged meat and fish products of Estonian origin at retail level

Toomas Kramarenko, Mati Roasto, Riikka Onerva Keto-Timonen, Mihkel Mäesaar, Kadrin Meremäe, Maiu Kuningas, Ari Jukka Antero Hörman, Hannu Juhani Korkeala

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The prevalence, counts and genetic diversity of Listeria monocytogenes in ready-to-eat (RTE) vacuum and modified atmosphere packaged meat and fish products was studied in Estonia. Within two consecutive years 370 RTE food samples were collected at retail level from which 11% were found to be positive for L. monocytogenes. Contamination was higher among RTE fish products (17%) than in RTE meat products (6%). Generally, the counts of L. monocytogenes in positive products remained under ten colony forming units (CFU) per gram of product. Only 1.6% of the RTE meat and fish products contained L. monocytogenes in range of 10-100 CFU/g and 0.3% more than 100 CFU/g at the end of shelf-life. The food category containing highest L. monocytogenes prevalence was RTE lightly salted fish products with the prevalence of 32%. Only one (03%) RTE food sample exceeded the 100 CFU/g food safety criterion set out in the EU Regulation 2073/2005. Pulsed-field gel electrophoresis (PFGE) characterization of the isolates showed an overall similarity higher than 70%, and nine clusters based on 100% similarity were revealed. PFGE genotyping revealed that the few predominant pulsotypes were associated with particular food plants.
Original languageEnglish
JournalFood Control
Volume67
Pages (from-to)48-52
Number of pages5
ISSN0956-7135
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 416 Food Science
  • 413 Veterinary science
  • Listeria monocytogenes
  • Prevalence
  • Counts
  • RTE meat products
  • RTE fish products

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