Immunohistochemical Staining of Histological Fragments Derived from Salivary Gland Tumour Fine-Needle Biopsy Aspirates

Marie Lundberg, Berent Munsterhjelm, Antti Mäkitie, Ilmo Leivo

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Objectives: The aim of this study was to describe a method for analysing histological fragments derived from fineneedle aspirate biopsy (FNAB) of salivary gland tumours (SGTs), and to evaluate the use of immunohistochemistry (IHC) on them. Study Design: We reviewed all 509 FNAB pathology reports taken from SGTs at Helsinki University Hospital, Finland, between 1999 and 2009. In 51% of the cases (n = 209) "histo-fragments" had been obtained and 31 had been further analysed by IHC. Of these, 25 (81%) were available for review. We evaluated the benefit of IHC by relating its added value to the preoperative cytological diagnosis and its accuracy compared with the postoperative histological diagnosis. Results: Most of the samples analysed by IHC were assigned a malignant diagnosis, with 12 different types of malignancy represented. IHC was advantageous in 76% of the cases. In the 108 studies using IHC in this series, antibodies to 36 different antigens were used. Conclusion: Analysis of histo-fragments in FNABs using IHC can be valuable in specific differential diagnostics and raises diagnostic accuracy in SGTs.
Original languageEnglish
JournalActa Cytologica
Issue number1
Pages (from-to)17-20
Number of pages4
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

Fields of Science

  • Salivary gland
  • Head and neck neoplasms
  • Fine-needle biopsy
  • Fine-needle aspirate
  • Immunohistochemistry
  • Histo-fragment
  • Histo-block
  • Cell block
  • 3111 Biomedicine

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