Impact of aspen furniture and restricted feeding on activity, blood pressure, heart rate and faecal corticosterone and immunoglobulin A excretion in rats (Rattus norvegicus) housed in individually ventilated cages

N. Kemppinen, J. Hau, A. Meller, K. Mauranen, T. Kohila, T. Nevalainen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

This study aims to evaluate the impact of adding different items in individually ventilated rat cages on the animal’s activity, cardiovascular parameters and faecal stress indicators. The following three cage items made of aspen were compared: a cross made of two intersecting boards, a similar cross where drilled holes were loaded with food pellets (restricted feeding) and a rectangular tube. Male rats of the strains BN and F344 (n ¼ 12) were housed in groups of three; one rat in each group was implanted with a telemetric transponder to measure mean arterial pressure (MAP) and heart rate (HR). In a crossover design, each group spent 14 days with each type of cage furniture, thereafter faecal pellets were collected for faecal analyses. The means of activity and means and coefficient of variation for MAP and HR were calculated for days 2, 6, 10 and 14.
As a way of determining which of the statistically significant MAP and HR mean changes were biologically meaningful, the night–day differences of the controls on day 14 were used. Both board types lowered MAP of F344 rats; hence dividing walls seem beneficial for F344 welfare. None of the MAP or HR differences in BN rats were biologically significant. No statistically significant differences in faecal corticosterone or IgA excretion were detected. In conclusion, provision of general
recommendations with respect to cage furniture for rat cages is complicated because there is a clear genetic component involved in how animals respond to these structures.
Original languageEnglish
JournalLaboratory Animals
Volume44
Pages (from-to)104-112
Number of pages9
ISSN0023-6772
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fields of Science

  • Refinement
  • reduction
  • rodents
  • environmental enrichment
  • telemetry
  • SPONTANEOUSLY HYPERTENSIVE-RATS
  • SPRAGUE-DAWLEY RATS
  • LABORATORY RATS
  • ENVIRONMENTAL ENRICHMENT
  • LOCOMOTOR-ACTIVITY
  • HOUSING CONDITIONS
  • DIFFERENT STRAINS
  • WISTAR RATS
  • BODY-WEIGHT
  • STRESS
  • 118 Biological sciences

Cite this

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title = "Impact of aspen furniture and restricted feeding on activity, blood pressure, heart rate and faecal corticosterone and immunoglobulin A excretion in rats (Rattus norvegicus) housed in individually ventilated cages",
abstract = "This study aims to evaluate the impact of adding different items in individually ventilated rat cages on the animal’s activity, cardiovascular parameters and faecal stress indicators. The following three cage items made of aspen were compared: a cross made of two intersecting boards, a similar cross where drilled holes were loaded with food pellets (restricted feeding) and a rectangular tube. Male rats of the strains BN and F344 (n ¼ 12) were housed in groups of three; one rat in each group was implanted with a telemetric transponder to measure mean arterial pressure (MAP) and heart rate (HR). In a crossover design, each group spent 14 days with each type of cage furniture, thereafter faecal pellets were collected for faecal analyses. The means of activity and means and coefficient of variation for MAP and HR were calculated for days 2, 6, 10 and 14.As a way of determining which of the statistically significant MAP and HR mean changes were biologically meaningful, the night–day differences of the controls on day 14 were used. Both board types lowered MAP of F344 rats; hence dividing walls seem beneficial for F344 welfare. None of the MAP or HR differences in BN rats were biologically significant. No statistically significant differences in faecal corticosterone or IgA excretion were detected. In conclusion, provision of generalrecommendations with respect to cage furniture for rat cages is complicated because there is a clear genetic component involved in how animals respond to these structures.",
keywords = "Refinement, reduction, rodents, environmental enrichment, telemetry, SPONTANEOUSLY HYPERTENSIVE-RATS, SPRAGUE-DAWLEY RATS, LABORATORY RATS, ENVIRONMENTAL ENRICHMENT, LOCOMOTOR-ACTIVITY, HOUSING CONDITIONS, DIFFERENT STRAINS, WISTAR RATS, BODY-WEIGHT, STRESS, Refinement, reduction, rodents, environmental enrichment, telemetry, 118 Biological sciences",
author = "N. Kemppinen and J. Hau and A. Meller and K. Mauranen and T. Kohila and T. Nevalainen",
year = "2010",
doi = "10.1258/la.2009.009058",
language = "English",
volume = "44",
pages = "104--112",
journal = "Laboratory Animals",
issn = "0023-6772",
publisher = "Royal Society of Medicine Services",

}

TY - JOUR

T1 - Impact of aspen furniture and restricted feeding on activity, blood pressure, heart rate and faecal corticosterone and immunoglobulin A excretion in rats (Rattus norvegicus) housed in individually ventilated cages

AU - Kemppinen, N.

AU - Hau, J.

AU - Meller, A.

AU - Mauranen, K.

AU - Kohila, T.

AU - Nevalainen, T.

PY - 2010

Y1 - 2010

N2 - This study aims to evaluate the impact of adding different items in individually ventilated rat cages on the animal’s activity, cardiovascular parameters and faecal stress indicators. The following three cage items made of aspen were compared: a cross made of two intersecting boards, a similar cross where drilled holes were loaded with food pellets (restricted feeding) and a rectangular tube. Male rats of the strains BN and F344 (n ¼ 12) were housed in groups of three; one rat in each group was implanted with a telemetric transponder to measure mean arterial pressure (MAP) and heart rate (HR). In a crossover design, each group spent 14 days with each type of cage furniture, thereafter faecal pellets were collected for faecal analyses. The means of activity and means and coefficient of variation for MAP and HR were calculated for days 2, 6, 10 and 14.As a way of determining which of the statistically significant MAP and HR mean changes were biologically meaningful, the night–day differences of the controls on day 14 were used. Both board types lowered MAP of F344 rats; hence dividing walls seem beneficial for F344 welfare. None of the MAP or HR differences in BN rats were biologically significant. No statistically significant differences in faecal corticosterone or IgA excretion were detected. In conclusion, provision of generalrecommendations with respect to cage furniture for rat cages is complicated because there is a clear genetic component involved in how animals respond to these structures.

AB - This study aims to evaluate the impact of adding different items in individually ventilated rat cages on the animal’s activity, cardiovascular parameters and faecal stress indicators. The following three cage items made of aspen were compared: a cross made of two intersecting boards, a similar cross where drilled holes were loaded with food pellets (restricted feeding) and a rectangular tube. Male rats of the strains BN and F344 (n ¼ 12) were housed in groups of three; one rat in each group was implanted with a telemetric transponder to measure mean arterial pressure (MAP) and heart rate (HR). In a crossover design, each group spent 14 days with each type of cage furniture, thereafter faecal pellets were collected for faecal analyses. The means of activity and means and coefficient of variation for MAP and HR were calculated for days 2, 6, 10 and 14.As a way of determining which of the statistically significant MAP and HR mean changes were biologically meaningful, the night–day differences of the controls on day 14 were used. Both board types lowered MAP of F344 rats; hence dividing walls seem beneficial for F344 welfare. None of the MAP or HR differences in BN rats were biologically significant. No statistically significant differences in faecal corticosterone or IgA excretion were detected. In conclusion, provision of generalrecommendations with respect to cage furniture for rat cages is complicated because there is a clear genetic component involved in how animals respond to these structures.

KW - Refinement

KW - reduction

KW - rodents

KW - environmental enrichment

KW - telemetry

KW - SPONTANEOUSLY HYPERTENSIVE-RATS

KW - SPRAGUE-DAWLEY RATS

KW - LABORATORY RATS

KW - ENVIRONMENTAL ENRICHMENT

KW - LOCOMOTOR-ACTIVITY

KW - HOUSING CONDITIONS

KW - DIFFERENT STRAINS

KW - WISTAR RATS

KW - BODY-WEIGHT

KW - STRESS

KW - Refinement, reduction, rodents, environmental enrichment, telemetry

KW - 118 Biological sciences

U2 - 10.1258/la.2009.009058

DO - 10.1258/la.2009.009058

M3 - Article

VL - 44

SP - 104

EP - 112

JO - Laboratory Animals

JF - Laboratory Animals

SN - 0023-6772

ER -