Implementation of improved postoperative care decreases the mortality rate of operated mice after an abundant 6-hydroxydopamine lesion of nigrostriatal dopaminergic neurons

Sini K. Koski, Sakari Leino, Saara Kaisa Rannanpää, Outi Susanna Salminen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

A mouse model of Parkinson’s disease with an abundant lesion of nigrostriatal dopaminergic
neurons can be achieved by stereotactic injection of 6-hydroxydopamine into the medial forebrain
bundle. However, postoperative mortality can be excessively high without intensive postoperative
care. Here, we show that improvements in stereotactic operations and postoperative
care result in significant benefits for both animal well-being and research efficiency. Adopting a
wide combination of mostly previously described improvements resulted in a decrease of postoperative
mortality from 71% to 14% and an increase in successful abundant dopaminergic
lesions from 46% to 81%. The techniques adopted are described in detail. In addition, we describe
a simple protocol for gradual preoperative handling which can be utilized to decrease
animal stress, aggressive and aversive behaviors, and to facilitate postoperative care and other
subsequent handling. We propose that the implementation of these improvements greatly decreases
the risk of animal suffering and that the improvements are worth adopting in any research
group utilizing abundant 6-hydroxydopamine-induced dopaminergic lesions in mice.
Suggestions for further improvement are also presented.
Original languageEnglish
JournalScandinavian journal of laboratory animal science
Volume45
Issue number1
Number of pages12
ISSN2002-0112
Publication statusAccepted/In press - 2019
MoE publication typeA1 Journal article-refereed

Cite this

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title = "Implementation of improved postoperative care decreases the mortality rate of operated mice after an abundant 6-hydroxydopamine lesion of nigrostriatal dopaminergic neurons",
abstract = "A mouse model of Parkinson’s disease with an abundant lesion of nigrostriatal dopaminergicneurons can be achieved by stereotactic injection of 6-hydroxydopamine into the medial forebrainbundle. However, postoperative mortality can be excessively high without intensive postoperativecare. Here, we show that improvements in stereotactic operations and postoperativecare result in significant benefits for both animal well-being and research efficiency. Adopting awide combination of mostly previously described improvements resulted in a decrease of postoperativemortality from 71{\%} to 14{\%} and an increase in successful abundant dopaminergiclesions from 46{\%} to 81{\%}. The techniques adopted are described in detail. In addition, we describea simple protocol for gradual preoperative handling which can be utilized to decreaseanimal stress, aggressive and aversive behaviors, and to facilitate postoperative care and othersubsequent handling. We propose that the implementation of these improvements greatly decreasesthe risk of animal suffering and that the improvements are worth adopting in any researchgroup utilizing abundant 6-hydroxydopamine-induced dopaminergic lesions in mice.Suggestions for further improvement are also presented.",
author = "Koski, {Sini K.} and Sakari Leino and Rannanp{\"a}{\"a}, {Saara Kaisa} and Salminen, {Outi Susanna}",
year = "2019",
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journal = "Scandinavian journal of laboratory animal science",
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publisher = "SCANDINAVIAN FEDERATION LABORATORY ANIMAL SCIENCE",
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Implementation of improved postoperative care decreases the mortality rate of operated mice after an abundant 6-hydroxydopamine lesion of nigrostriatal dopaminergic neurons. / Koski, Sini K.; Leino, Sakari; Rannanpää, Saara Kaisa; Salminen, Outi Susanna.

In: Scandinavian journal of laboratory animal science, Vol. 45, No. 1, 2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

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AU - Leino, Sakari

AU - Rannanpää, Saara Kaisa

AU - Salminen, Outi Susanna

PY - 2019

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AB - A mouse model of Parkinson’s disease with an abundant lesion of nigrostriatal dopaminergicneurons can be achieved by stereotactic injection of 6-hydroxydopamine into the medial forebrainbundle. However, postoperative mortality can be excessively high without intensive postoperativecare. Here, we show that improvements in stereotactic operations and postoperativecare result in significant benefits for both animal well-being and research efficiency. Adopting awide combination of mostly previously described improvements resulted in a decrease of postoperativemortality from 71% to 14% and an increase in successful abundant dopaminergiclesions from 46% to 81%. The techniques adopted are described in detail. In addition, we describea simple protocol for gradual preoperative handling which can be utilized to decreaseanimal stress, aggressive and aversive behaviors, and to facilitate postoperative care and othersubsequent handling. We propose that the implementation of these improvements greatly decreasesthe risk of animal suffering and that the improvements are worth adopting in any researchgroup utilizing abundant 6-hydroxydopamine-induced dopaminergic lesions in mice.Suggestions for further improvement are also presented.

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