Improved diastolic function after myoblast transplantation in a model of ischemia-infarction

Tommi Pätilä, Tuija Ikonen, Esko Kankuri, Aki Uutela, Jyri Lommi, Leena Krogerus, Pertteli Salmenperä, Josef Bizik, Kirsi Lauerma, Ari Harjula

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Objectives. The aim of this study was to evaluate the effect of myoblast transplantation on left ventricular function, perfusion, and scar formation after compromised coronary flow. Design. A coronary vessel with Ameroid-induced stenosis was ligated and skeletal muscle was biopsied for isolation and cultivation of myoblasts. Two weeks after ligation, animals were randomly selected to receive intramyocardial injections of 2106 myoblasts or vehicle. Fifteen animals survived the whole study period (n=9 and n=6, respectively). All animals underwent cardiac magnetic resonance imaging (MRI) and angiography pretreatment and four weeks posttreatment. Results. Peak filling rate of the left ventricle improved in the myoblast group (p=0.0048), but not in the control group. Peak ejection rate and duration of diastole improved only in the myoblast group (p=0.049 and p=0.0039, respectively). Ejection fraction or local thickening did not change. Fibrosis and perfusion were similar in both groups, but more microvessels were present histologically in the myoblast group. Conclusions. In this preclinical study, autologous myoblast transplantation improved ischemic heart function via enhanced diastolic filling of the left ventricle.
Original languageEnglish
JournalScandinavian Cardiovascular Journal
Volume43
Issue number2
Pages (from-to)100-109
Number of pages10
ISSN1401-7431
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 311 Basic medicine

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