Projects per year
Abstract
Myocardial infarction results in a massive loss of cardiomyocytes (CMs). Unfortunately, current therapies are unsuccessful in replacing lost CMs, and thus, there is an urgent need for innovative approaches. Here, a nanosystem based on spermine-acetalated dextran (AcDXSp) and encapsulating two drug compounds able to stimulate in vitro CMs proliferation is developed. The nanosystem is coated by deposition of a film constituted by tannic acid (TA) and Fe3+ ions. The coating with TA increases the retention of the nanocarrier in cell co-cultures of CMs and fibroblasts stimulated with transforming growth factor (TGF)-β, due to the high affinity of TA for components of the cardiac extracellular matrix. The system exhibits biocompatibility toward primary CMs and induces their proliferation, as indicated by the two-fold increase of CMs in the active cell cycle. At the same time, the presence of TA synergistically helps contrasting fibrosis by reducing profibrotic genes expression, such as collagen 1 and osteopontin, by approximately 80% compared to the control. Overall, the developed nanosystem demonstrates the capability to stimulate CMs proliferation and reduce fibrosis, showing potential benefits for future in vivo applications.
Original language | English |
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Article number | 2109032 |
Journal | Advanced Functional Materials |
Volume | 32 |
Issue number | 5 |
Number of pages | 16 |
ISSN | 1616-3028 |
DOIs | |
Publication status | Published - 26 Jan 2022 |
MoE publication type | A1 Journal article-refereed |
Fields of Science
- 1182 Biochemistry, cell and molecular biology
- 317 Pharmacy
- cardiac regeneration
- fibrosis
- heart targeting
- proliferation
- spermine-acetalated dextran nanoparticles
- tannic acid
- MYOCARDIAL-INFARCTION
- CARDIOMYOCYTE PROLIFERATION
- ISCHEMIC-MYOCARDIUM
- CELLULAR UPTAKE
- ANGIOTENSIN-II
- HISTONE H3
- HEART
- INHIBITOR
- KINASE
- CELLS
- 318 Medical biotechnology
Projects
- 1 Finished
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BIOMIMETIC PROTOCELLS TO RESTORE INJURED CELLS IN HEART: TOWARDS PERSONALIZED MEDICINE (PROTO-CELL)
Torrieri, G. (Participant), Balasubramanian, V. (Project manager) & Santos, H. A. (Principal Investigator)
01/08/2018 → 31/07/2021
Project: University of Helsinki Three-Year Research Project