Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment

Aleksandra Steć; Monika Targońska; Shishir Jaikishan; Rui Chen; Piotr Mucha; Grzegorz Czyrski; Jacek Jasiecki; Agata Płoska; Andrea Heinz; Susanne  Wiedmer; Leszek Kalinowski; Krzysztof Waleron; Bartosz Wielgomas; Szymon Dziomba

doi:10.1080/10717544.2024.2439272

Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment

Aleksandra Steć, Monika Targońska, Shishir Jaikishan, Rui Chen, Piotr Mucha, Grzegorz Czyrski, Jacek Jasiecki, Agata Płoska, Andrea Heinz, Susanne Wiedmer, Leszek Kalinowski, Krzysztof Waleron, Bartosz Wielgomas, Szymon Dziomba

Department of Chemistry

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources of EVs are currently being explored to overcome limitations related to their manufacturing from mesenchymal stem cells. In this work, Citrus limon-derived EVs were tested as carriers for the widely used chemotherapeutic drug - doxorubicin (DOX). Capillary electrophoresis (CE) and nanoplasmonic sensing (NPS) were developed for the quality control of DOX-EV preparations. It was found that the CE method enables simultaneous detection of free and incorporated DOX and allows assessing the stability of the preparations and the drug leakage. NPS, on the other hand, demonstrated that DOX is accumulated in the interfacial region of the carrier. The activity of DOX-loaded EVs was tested on HeLa (cervical cancer cells) and HEK293T (human embryonic kidney cells) cell lines. It was found that DOX incorporation into plant-derived EVs virtually does not affect the drug's cytotoxicity to HeLa cells but significantly decreases DOX activity against HEK293T cell line.

Original language	English
Article number	2439272
Journal	Drug Delivery
Volume	32
Issue number	1
Pages (from-to)	2439272
Number of pages	11
ISSN	1071-7544
DOIs	https://doi.org/10.1080/10717544.2024.2439272
Publication status	Published - 11 Dec 2024
MoE publication type	A1 Journal article-refereed

Fields of Science

1182 Biochemistry, cell and molecular biology
3111 Biomedicine
317 Pharmacy

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10.1080/10717544.2024.2439272Licence: CC BY

12. Stec-2025-Drug Deli-DoxorubicinFinal published version, 7.01 MBLicence: CC BY

Jaikishan-Walter and Lisi Wahl Foundation
Jaikishan, S. (Project manager)
Walter och Lisi Wahls stiftelse för naturvetenskaplig forskning sr Nordea/2743 Säätiö/kirjanpitopalv.
12/02/2024 → 11/01/2025
Project: Foundations (Private Foundations, Non-Profit Foundations, Charitable Trusts)

Cite this

Steć, A., Targońska, M., Jaikishan, S., Chen, R., Mucha, P., Czyrski, G., Jasiecki, J., Płoska, A., Heinz, A., Wiedmer, S., Kalinowski, L., Waleron, K., Wielgomas, B., & Dziomba, S. (2024). Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment. Drug Delivery, 32(1), 2439272. Article 2439272. https://doi.org/10.1080/10717544.2024.2439272

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abstract = "Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources of EVs are currently being explored to overcome limitations related to their manufacturing from mesenchymal stem cells. In this work, Citrus limon-derived EVs were tested as carriers for the widely used chemotherapeutic drug - doxorubicin (DOX). Capillary electrophoresis (CE) and nanoplasmonic sensing (NPS) were developed for the quality control of DOX-EV preparations. It was found that the CE method enables simultaneous detection of free and incorporated DOX and allows assessing the stability of the preparations and the drug leakage. NPS, on the other hand, demonstrated that DOX is accumulated in the interfacial region of the carrier. The activity of DOX-loaded EVs was tested on HeLa (cervical cancer cells) and HEK293T (human embryonic kidney cells) cell lines. It was found that DOX incorporation into plant-derived EVs virtually does not affect the drug's cytotoxicity to HeLa cells but significantly decreases DOX activity against HEK293T cell line.",

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Steć, A, Targońska, M, Jaikishan, S, Chen, R, Mucha, P, Czyrski, G, Jasiecki, J, Płoska, A, Heinz, A, Wiedmer, S, Kalinowski, L, Waleron, K, Wielgomas, B & Dziomba, S 2024, 'Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment', Drug Delivery, vol. 32, no. 1, 2439272, pp. 2439272. https://doi.org/10.1080/10717544.2024.2439272

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T1 - Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment

AU - Steć, Aleksandra

AU - Targońska, Monika

AU - Jaikishan, Shishir

AU - Chen, Rui

AU - Mucha, Piotr

AU - Czyrski, Grzegorz

AU - Jasiecki, Jacek

AU - Płoska, Agata

AU - Heinz, Andrea

AU - Wiedmer, Susanne

AU - Kalinowski, Leszek

AU - Waleron, Krzysztof

AU - Wielgomas, Bartosz

AU - Dziomba, Szymon

PY - 2024/12/11

Y1 - 2024/12/11

N2 - Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources of EVs are currently being explored to overcome limitations related to their manufacturing from mesenchymal stem cells. In this work, Citrus limon-derived EVs were tested as carriers for the widely used chemotherapeutic drug - doxorubicin (DOX). Capillary electrophoresis (CE) and nanoplasmonic sensing (NPS) were developed for the quality control of DOX-EV preparations. It was found that the CE method enables simultaneous detection of free and incorporated DOX and allows assessing the stability of the preparations and the drug leakage. NPS, on the other hand, demonstrated that DOX is accumulated in the interfacial region of the carrier. The activity of DOX-loaded EVs was tested on HeLa (cervical cancer cells) and HEK293T (human embryonic kidney cells) cell lines. It was found that DOX incorporation into plant-derived EVs virtually does not affect the drug's cytotoxicity to HeLa cells but significantly decreases DOX activity against HEK293T cell line.

AB - Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources of EVs are currently being explored to overcome limitations related to their manufacturing from mesenchymal stem cells. In this work, Citrus limon-derived EVs were tested as carriers for the widely used chemotherapeutic drug - doxorubicin (DOX). Capillary electrophoresis (CE) and nanoplasmonic sensing (NPS) were developed for the quality control of DOX-EV preparations. It was found that the CE method enables simultaneous detection of free and incorporated DOX and allows assessing the stability of the preparations and the drug leakage. NPS, on the other hand, demonstrated that DOX is accumulated in the interfacial region of the carrier. The activity of DOX-loaded EVs was tested on HeLa (cervical cancer cells) and HEK293T (human embryonic kidney cells) cell lines. It was found that DOX incorporation into plant-derived EVs virtually does not affect the drug's cytotoxicity to HeLa cells but significantly decreases DOX activity against HEK293T cell line.

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KW - 3111 Biomedicine

KW - 317 Pharmacy

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JO - Drug Delivery

JF - Drug Delivery

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Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment

Abstract

Fields of Science

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Projects

Jaikishan-Walter and Lisi Wahl Foundation

Cite this