Abstract
Biohybrid nanosystems represent the cutting‐edge research in biofunctionalization of micro‐ and nano‐systems. Their physicochemical properties bring along advantages in the circulation time, camouflaging from the phagocytes, and novel antigens. This is partially a result of the qualitative differences in the protein corona, and the preferential targeting and uptake in homologous cells. However, the effect of the cell membrane on the cellular endocytosis mechanisms and time has not been fully evaluated yet. Here, the effect is assessed by quantitative flow cytometry analysis on the endocytosis of hydrophilic, negatively charged porous silicon nanoparticles and on their membrane‐coated counterparts, in the presence of chemical inhibitors of different uptake pathways. Principal component analysis is used to analyze all the data and extrapolate patterns to highlight the cell‐specific differences in the endocytosis mechanisms. Furthermore, the differences in the composition of static protein corona between naked and coated particles are investigated together with how these differences affect the interaction with human macrophages. Overall, the presence of the cell membrane only influences the speed and the entity of nanoparticles association with the cells, while there is no direct effect on the endocytosis pathways, composition of protein corona, or any reduction in macrophage‐mediated uptake.
Original language | English |
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Article number | 2000529 |
Journal | Advanced Healthcare Materials |
Volume | 9 |
Issue number | 17 |
Number of pages | 9 |
ISSN | 2192-2640 |
DOIs | |
Publication status | Published - 9 Sept 2020 |
MoE publication type | A1 Journal article-refereed |
Fields of Science
- COATED NANOPARTICLES
- DRUG-DELIVERY
- IMPACT
- VIVO PROTEIN CORONA
- biohybrids
- cancer cell membranes
- nanoparticle uptake
- nanoparticles
- protein corona
- 3111 Biomedicine
- 318 Medical biotechnology
- 221 Nano-technology