Influence of growth conditions on the nisin production of bioengineered Lactococcus lactis strains

Oe. Simsek, A. H. Con, N. Akkoc, P. E. J. Saris, Mustafa Akcelik

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Nisin production of three bioengineered strains, (LAC338, LAC339 and LAC340) with immunity (nisFEG) and/or regulation (nisRK) genes of nisin biosynthesis on plasmids in the Lactococcus lactis LL27 nisin producer, was evaluated under pH-controlled and pH-uncontrolled batch fermentations. Optimization studies showed that fructose and yeast extract yielded the highest nisin activity. The strains LAC338, LAC339, and LAC340 produced 24, 45, and 44% more nisin, respectively, than wild-type L. lactis LL27 after 12-h incubation. However, sharp decreases in the yield of nisin were observed at the late phase of fermentation with LAC339 and LL27 in contrast to LAC340 and LAC338 strains for which the high level of nisin could be maintained longer. Obviously, increasing the copy number of the regulation genes together with immunity genes in the nisin producers retarded the loss of nisin in the late phase of the fermentation.
Original languageEnglish
JournalJournal of Industrial Microbiology and Biotechnology
Volume36
Issue number4
Pages (from-to)481-490
Number of pages10
ISSN1367-5435
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fields of Science

  • Nisin fermentation
  • Lactococcus lactis
  • Nisin immunity
  • Nisin regulation
  • Growth parameters
  • SUBSP LACTIS
  • BATCH FERMENTATIONS
  • BACILLUS-SUBTILIS
  • BIOSYNTHESIS
  • IMMUNITY
  • CULTURES
  • GENES
  • A164
  • N8
  • 118 Biological sciences

Cite this

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title = "Influence of growth conditions on the nisin production of bioengineered Lactococcus lactis strains",
abstract = "Nisin production of three bioengineered strains, (LAC338, LAC339 and LAC340) with immunity (nisFEG) and/or regulation (nisRK) genes of nisin biosynthesis on plasmids in the Lactococcus lactis LL27 nisin producer, was evaluated under pH-controlled and pH-uncontrolled batch fermentations. Optimization studies showed that fructose and yeast extract yielded the highest nisin activity. The strains LAC338, LAC339, and LAC340 produced 24, 45, and 44{\%} more nisin, respectively, than wild-type L. lactis LL27 after 12-h incubation. However, sharp decreases in the yield of nisin were observed at the late phase of fermentation with LAC339 and LL27 in contrast to LAC340 and LAC338 strains for which the high level of nisin could be maintained longer. Obviously, increasing the copy number of the regulation genes together with immunity genes in the nisin producers retarded the loss of nisin in the late phase of the fermentation.",
keywords = "Nisin fermentation, Lactococcus lactis, Nisin immunity, Nisin regulation, Growth parameters, SUBSP LACTIS, BATCH FERMENTATIONS, BACILLUS-SUBTILIS, BIOSYNTHESIS, IMMUNITY, CULTURES, GENES, A164, N8, 118 Biological sciences",
author = "Oe. Simsek and Con, {A. H.} and N. Akkoc and Saris, {P. E. J.} and Mustafa Akcelik",
year = "2009",
doi = "10.1007/s10295-008-0517-4",
language = "English",
volume = "36",
pages = "481--490",
journal = "Journal of Industrial Microbiology and Biotechnology",
issn = "1367-5435",
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Influence of growth conditions on the nisin production of bioengineered Lactococcus lactis strains. / Simsek, Oe.; Con, A. H.; Akkoc, N.; Saris, P. E. J.; Akcelik, Mustafa.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 36, No. 4, 2009, p. 481-490.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Influence of growth conditions on the nisin production of bioengineered Lactococcus lactis strains

AU - Simsek, Oe.

AU - Con, A. H.

AU - Akkoc, N.

AU - Saris, P. E. J.

AU - Akcelik, Mustafa

PY - 2009

Y1 - 2009

N2 - Nisin production of three bioengineered strains, (LAC338, LAC339 and LAC340) with immunity (nisFEG) and/or regulation (nisRK) genes of nisin biosynthesis on plasmids in the Lactococcus lactis LL27 nisin producer, was evaluated under pH-controlled and pH-uncontrolled batch fermentations. Optimization studies showed that fructose and yeast extract yielded the highest nisin activity. The strains LAC338, LAC339, and LAC340 produced 24, 45, and 44% more nisin, respectively, than wild-type L. lactis LL27 after 12-h incubation. However, sharp decreases in the yield of nisin were observed at the late phase of fermentation with LAC339 and LL27 in contrast to LAC340 and LAC338 strains for which the high level of nisin could be maintained longer. Obviously, increasing the copy number of the regulation genes together with immunity genes in the nisin producers retarded the loss of nisin in the late phase of the fermentation.

AB - Nisin production of three bioengineered strains, (LAC338, LAC339 and LAC340) with immunity (nisFEG) and/or regulation (nisRK) genes of nisin biosynthesis on plasmids in the Lactococcus lactis LL27 nisin producer, was evaluated under pH-controlled and pH-uncontrolled batch fermentations. Optimization studies showed that fructose and yeast extract yielded the highest nisin activity. The strains LAC338, LAC339, and LAC340 produced 24, 45, and 44% more nisin, respectively, than wild-type L. lactis LL27 after 12-h incubation. However, sharp decreases in the yield of nisin were observed at the late phase of fermentation with LAC339 and LL27 in contrast to LAC340 and LAC338 strains for which the high level of nisin could be maintained longer. Obviously, increasing the copy number of the regulation genes together with immunity genes in the nisin producers retarded the loss of nisin in the late phase of the fermentation.

KW - Nisin fermentation

KW - Lactococcus lactis

KW - Nisin immunity

KW - Nisin regulation

KW - Growth parameters

KW - SUBSP LACTIS

KW - BATCH FERMENTATIONS

KW - BACILLUS-SUBTILIS

KW - BIOSYNTHESIS

KW - IMMUNITY

KW - CULTURES

KW - GENES

KW - A164

KW - N8

KW - 118 Biological sciences

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