Intracellular localization and effects of individually expressed human parechovirus 1 non-structural proteins

Camilla Krogerus, Olga Samuilova, Tuija Pöyry, Eija Jokitalo, Timo Hyypiä

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Human parechovirus 1 (HPEV-1) has many unique features compared with other picornaviruses and it has been shown that the replication complex formed during HPEV-1 infection is different from that of other picornaviruses. Here, the intracellular localization and functional effects of individually expressed HPEV-1 non-structural proteins were studied. The 2A and 3D proteins were found diffusely in the cytoplasm and nucleus of the cell. The 3A and 3AB proteins were observed to co-localize with the markers for the Golgi apparatus, whereas 2B co-localized with markers for the endoplasmic reticulum and the 2C and 2BC proteins were observed mainly on the surface of lipid droplets. The 2C protein, which has been implicated in replication-complex formation in enterovirus-infected cells, was not able to induce vesicles similar to those seen in HPEV-1-infected cells when expressed individually. However, in superinfected cells, the fusion protein was able to relocate to the virus replication complexes. Similar to other picornaviruses, HPEV-1 was found to interfere with cellular secretion, but this function could not be ascribed to any of the individually expressed non-structural proteins.
Original languageEnglish
JournalJournal of General Virology
Volume88
Pages (from-to)831-841
Number of pages11
ISSN0022-1317
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Cite this

Krogerus, Camilla ; Samuilova, Olga ; Pöyry, Tuija ; Jokitalo, Eija ; Hyypiä, Timo. / Intracellular localization and effects of individually expressed human parechovirus 1 non-structural proteins. In: Journal of General Virology. 2007 ; Vol. 88. pp. 831-841.
@article{08250561f2c64405a7f7c4a834baf687,
title = "Intracellular localization and effects of individually expressed human parechovirus 1 non-structural proteins",
abstract = "Human parechovirus 1 (HPEV-1) has many unique features compared with other picornaviruses and it has been shown that the replication complex formed during HPEV-1 infection is different from that of other picornaviruses. Here, the intracellular localization and functional effects of individually expressed HPEV-1 non-structural proteins were studied. The 2A and 3D proteins were found diffusely in the cytoplasm and nucleus of the cell. The 3A and 3AB proteins were observed to co-localize with the markers for the Golgi apparatus, whereas 2B co-localized with markers for the endoplasmic reticulum and the 2C and 2BC proteins were observed mainly on the surface of lipid droplets. The 2C protein, which has been implicated in replication-complex formation in enterovirus-infected cells, was not able to induce vesicles similar to those seen in HPEV-1-infected cells when expressed individually. However, in superinfected cells, the fusion protein was able to relocate to the virus replication complexes. Similar to other picornaviruses, HPEV-1 was found to interfere with cellular secretion, but this function could not be ascribed to any of the individually expressed non-structural proteins.",
author = "Camilla Krogerus and Olga Samuilova and Tuija P{\"o}yry and Eija Jokitalo and Timo Hyypi{\"a}",
year = "2007",
doi = "10.1099/vir.0.82201.-0",
language = "English",
volume = "88",
pages = "831--841",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "American Society for Microbiology",

}

Intracellular localization and effects of individually expressed human parechovirus 1 non-structural proteins. / Krogerus, Camilla; Samuilova, Olga; Pöyry, Tuija; Jokitalo, Eija; Hyypiä, Timo.

In: Journal of General Virology, Vol. 88, 2007, p. 831-841.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Intracellular localization and effects of individually expressed human parechovirus 1 non-structural proteins

AU - Krogerus, Camilla

AU - Samuilova, Olga

AU - Pöyry, Tuija

AU - Jokitalo, Eija

AU - Hyypiä, Timo

PY - 2007

Y1 - 2007

N2 - Human parechovirus 1 (HPEV-1) has many unique features compared with other picornaviruses and it has been shown that the replication complex formed during HPEV-1 infection is different from that of other picornaviruses. Here, the intracellular localization and functional effects of individually expressed HPEV-1 non-structural proteins were studied. The 2A and 3D proteins were found diffusely in the cytoplasm and nucleus of the cell. The 3A and 3AB proteins were observed to co-localize with the markers for the Golgi apparatus, whereas 2B co-localized with markers for the endoplasmic reticulum and the 2C and 2BC proteins were observed mainly on the surface of lipid droplets. The 2C protein, which has been implicated in replication-complex formation in enterovirus-infected cells, was not able to induce vesicles similar to those seen in HPEV-1-infected cells when expressed individually. However, in superinfected cells, the fusion protein was able to relocate to the virus replication complexes. Similar to other picornaviruses, HPEV-1 was found to interfere with cellular secretion, but this function could not be ascribed to any of the individually expressed non-structural proteins.

AB - Human parechovirus 1 (HPEV-1) has many unique features compared with other picornaviruses and it has been shown that the replication complex formed during HPEV-1 infection is different from that of other picornaviruses. Here, the intracellular localization and functional effects of individually expressed HPEV-1 non-structural proteins were studied. The 2A and 3D proteins were found diffusely in the cytoplasm and nucleus of the cell. The 3A and 3AB proteins were observed to co-localize with the markers for the Golgi apparatus, whereas 2B co-localized with markers for the endoplasmic reticulum and the 2C and 2BC proteins were observed mainly on the surface of lipid droplets. The 2C protein, which has been implicated in replication-complex formation in enterovirus-infected cells, was not able to induce vesicles similar to those seen in HPEV-1-infected cells when expressed individually. However, in superinfected cells, the fusion protein was able to relocate to the virus replication complexes. Similar to other picornaviruses, HPEV-1 was found to interfere with cellular secretion, but this function could not be ascribed to any of the individually expressed non-structural proteins.

U2 - 10.1099/vir.0.82201.-0

DO - 10.1099/vir.0.82201.-0

M3 - Article

VL - 88

SP - 831

EP - 841

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

ER -