Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide

Thomas McWilliams, Ian G. Ganley

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

Abstract

Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.
Original languageEnglish
Title of host publicationAutophagy : Methods and Protocols
EditorsNicholas Ktistakis, Oliver Florey
Number of pages22
PublisherSpringer Nature
Publication date5 Jan 2019
Edition1st ed.
Pages621-642
ISBN (Print)978-1-4939-8872-3
ISBN (Electronic)978-1-4939-8873-0
DOIs
Publication statusPublished - 5 Jan 2019
MoE publication typeA3 Book chapter

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, Inc.
Number1880
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fields of Science

  • 3112 Neurosciences
  • NEURODEGENERATION
  • 1182 Biochemistry, cell and molecular biology
  • MITOPHAGY
  • AUTOPHAGY
  • Mitochondria
  • 3111 Biomedicine
  • METABOLISM
  • 1184 Genetics, developmental biology, physiology
  • MOUSE MODELS
  • DEVELOPMENTAL BIOLOGY

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