@inbook{1617362b3cf84fe08c14d7a1c0eefd23,
title = "Investigating Mitophagy and Mitochondrial Morphology In Vivo Using mito-QC: A Comprehensive Guide",
abstract = "Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.",
keywords = "3112 Neurosciences, NEURODEGENERATION, 1182 Biochemistry, cell and molecular biology, MITOPHAGY, AUTOPHAGY, Mitochondria, 3111 Biomedicine, METABOLISM, 1184 Genetics, developmental biology, physiology, MOUSE MODELS, DEVELOPMENTAL BIOLOGY",
author = "Thomas McWilliams and Ganley, {Ian G.}",
year = "2019",
month = jan,
day = "5",
doi = "10.1007/978-1-4939-8873-0_41",
language = "English",
isbn = "978-1-4939-8872-3",
series = "Methods in Molecular Biology",
publisher = "Springer",
number = "1880",
pages = "621--642",
editor = "Nicholas Ktistakis and Oliver Florey",
booktitle = "Autophagy",
address = "United States",
edition = "1st ed.",
}